Construct: sgRNA BRDN0001146118
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGCTCTACTTACGGGGTCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- AK7 (122481)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77914
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
96408937 |
- |
AK7 |
NNNTCTACTTACGGGGTCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
149322473 |
+ |
TM4SF18 |
NNNTCTACATACTGGGTCCA |
NGG |
2 |
0.2786 |
Tier II |
| 3 |
NC_000023.11 |
X |
41569295 |
- |
CASK |
NNNACTACTTACGGGGACCA |
NGG |
2 |
0.254 |
Tier II |
| 4 |
NC_000003.12 |
3 |
149322473 |
+ |
TM4SF18-AS1 |
NNNTCTACATACTGGGTCCA |
NGG |
2 |
0.2786 |
Tier III |
| 5 |
NC_000007.14 |
7 |
86978 |
- |
LOC105375113 |
NNNTCTAATTACTGGGTCCA |
NGG |
2 |
0.195 |
Tier III |
| 6 |
NC_000005.10 |
5 |
61697429 |
+ |
LINC03122 |
NNNTCTCCTTAGGGGGTCCA |
NGG |
2 |
0.1944 |
Tier III |
| 7 |
NC_000005.10 |
5 |
61697429 |
+ |
LOC101928651 |
NNNTCTCCTTAGGGGGTCCA |
NGG |
2 |
0.1944 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
105716075 |
- |
Ak7 |
NNNTCTACTTACAGGGTCCA |
NGG |
1 |
0.9231 |
Tier I |
| 2 |
NC_000077.6 |
11 |
102855780 |
- |
Eftud2 |
NNNTCTACTTACGAGGTCCA |
NGC |
2 |
0.0167 |
Tier II |
| 3 |
NC_000069.6 |
3 |
142713738 |
+ |
Kyat3 |
NNNTCTACTTATGGGGTCCA |
NGC |
2 |
0.012 |
Tier II |
Other clones with same target sequence:
(none)
