Construct: sgRNA BRDN0001146119
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAATCGCCACCGACCAACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- WNK3 (65267)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76925
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
54250105 |
+ |
WNK3 |
NNNTCGCCACCGACCAACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
234215861 |
- |
SLC35F3 |
NNNTCACCACCTACCAACTG |
NGG |
2 |
0.3846 |
Tier II |
| 3 |
NC_000014.9 |
14 |
58298698 |
- |
ARID4A |
NNNTCCCCACCTACCAACTG |
NGG |
2 |
0.2622 |
Tier II |
| 4 |
NC_000008.11 |
8 |
7803893 |
- |
LOC124901865 |
NNNTCCCCACCGGCCAACTG |
NGG |
2 |
0.4447 |
Tier III |
| 5 |
NC_000001.11 |
1 |
1127963 |
+ |
LOC124903819 |
NNNTCGCCACCGCCCCACTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
151285806 |
- |
Wnk3 |
NNNCCGCCAGCGACCAACTG |
NGG |
2 |
0.2516 |
Tier I |
| 2 |
NC_000082.6 |
16 |
35651724 |
- |
Sema5b |
NNNCCGCCACAGACCAACTG |
NGG |
2 |
0.4853 |
Tier II |
| 3 |
NC_000071.6 |
5 |
53137191 |
+ |
Sel1l3 |
NNNTAGCCACAGACCAACTG |
NGG |
2 |
0.4286 |
Tier II |
| 4 |
NC_000080.6 |
14 |
62943237 |
- |
Wdfy2 |
NNNTAGCCACTGACCAACTG |
NGG |
2 |
0.1758 |
Tier II |
| 5 |
NC_000069.6 |
3 |
104789099 |
- |
Rhoc |
NNNTCGCCACCCACCAGCTG |
NGG |
2 |
0.0934 |
Tier II |
Other clones with same target sequence:
(none)
