Construct: sgRNA BRDN0001146124
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATATGCTATACATTACCGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKK (139189)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75844
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
50424248 |
+ |
DGKK |
NNNATGCTATACATTACCGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
12264998 |
+ |
FRMPD4 |
NNNATGCTGTACATTACCAC |
NGG |
2 |
0.4762 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
82706922 |
+ |
Col4a3 |
NNNAAGCAATACATTACCGC |
NGG |
2 |
0.4 |
Tier II |
| 2 |
NC_000073.6 |
7 |
80640830 |
+ |
Crtc3 |
NNNATGGTATACATTACAGC |
NGG |
2 |
0.2534 |
Tier II |
| 3 |
NC_000083.6 |
17 |
13831970 |
- |
Afdn |
NNNATGCTATACATGAGCGC |
NGG |
2 |
0.0029 |
Tier II |
| 4 |
NC_000067.6 |
1 |
82706922 |
+ |
C430014B12Rik |
NNNAAGCAATACATTACCGC |
NGG |
2 |
0.4 |
Tier III |
| 5 |
NC_000073.6 |
7 |
80640830 |
+ |
Gm15880 |
NNNATGGTATACATTACAGC |
NGG |
2 |
0.2534 |
Tier III |
| 6 |
NC_000077.6 |
11 |
71570109 |
+ |
9230020A06Rik |
NNNATGCTATACATTACTGC |
NAG |
2 |
0.1667 |
Tier III |
| 7 |
NC_000077.6 |
11 |
71570109 |
+ |
Gm33351 |
NNNATGCTATACATTACTGC |
NAG |
2 |
0.1667 |
Tier III |
| 8 |
NC_000081.6 |
15 |
57626821 |
+ |
Gm35682 |
NNNATGCTATACATTACCAC |
NGC |
2 |
0.0159 |
Tier III |
Other clones with same target sequence:
(none)
