Construct: sgRNA BRDN0001146126
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGGGGGACTACAACGTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CSNK1D (1453)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76715
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
82255530 |
- |
CSNK1D |
NNNGGGGGACTACAACGTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000022.11 |
22 |
38302962 |
- |
CSNK1E |
NNNGGGCGACTACAACGTGA |
NGG |
2 |
0.0859 |
Tier I |
| 3 |
NC_000022.11 |
22 |
38302962 |
- |
TPTEP2-CSNK1E |
NNNGGGCGACTACAACGTGA |
NGG |
2 |
0.0859 |
Tier I |
| 4 |
NC_000003.12 |
3 |
189878659 |
- |
TP63 |
NNNGGGGGATCACAACGTCA |
NGG |
2 |
0.6275 |
Tier II |
| 5 |
NC_000008.11 |
8 |
109300065 |
+ |
NUDCD1 |
NNNGGGAGACTACTACGTCA |
NGG |
2 |
0.5333 |
Tier II |
| 6 |
NC_000001.11 |
1 |
224693684 |
+ |
CNIH3 |
NNNGGGGGACTACAAAGTTA |
NGG |
2 |
0.4615 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
120973922 |
- |
Csnk1d |
NNNGGGGGACTACAATGTCA |
NGG |
1 |
0.3077 |
Tier I |
| 2 |
NC_000072.6 |
6 |
8674919 |
- |
Ica1 |
NNNGGGAGACTACAAAGTCA |
NGG |
2 |
1.0 |
Tier II |
| 3 |
NC_000085.6 |
19 |
53755199 |
+ |
Rbm20 |
NNNGAGGGACTACAAAGTCA |
NGG |
2 |
0.8667 |
Tier II |
| 4 |
NC_000085.6 |
19 |
55749609 |
+ |
Tcf7l2 |
NNNGGGGGACGACAACGACA |
NGG |
2 |
0.2667 |
Tier II |
| 5 |
NC_000070.6 |
4 |
132128683 |
- |
Oprd1 |
NNNGGAGGACTACAAGGTCA |
NGG |
2 |
0.1538 |
Tier II |
| 6 |
NC_000085.6 |
19 |
53755199 |
+ |
Gm30943 |
NNNGAGGGACTACAAAGTCA |
NGG |
2 |
0.8667 |
Tier III |
| 7 |
NC_000072.6 |
6 |
69514525 |
- |
Igk |
NNNGGGGGACTACAAAGTCT |
NGG |
2 |
0.6 |
Tier III |
Other clones with same target sequence:
(none)
