Construct: sgRNA BRDN0001146135
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTTGCATATAAGCAACGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAN3 (255967)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77424
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
28260489 |
- |
PAN3 |
NNNTGCATATAAGCAACGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
113585295 |
+ |
CAMK2D |
NNNTTCATATAATCAACGTG |
NGG |
2 |
0.09 |
Tier II |
| 3 |
NC_000006.12 |
6 |
144352405 |
- |
UTRN |
NNNTGCATATAAGCAAAGTG |
NGA |
2 |
0.0324 |
Tier II |
| 4 |
NC_000012.12 |
12 |
62620722 |
+ |
MIRLET7IHG |
NNNAGCATATAAGCAACTTG |
NGG |
2 |
0.3175 |
Tier III |
| 5 |
NC_000001.11 |
1 |
236927683 |
+ |
LOC105373218 |
NNNTGCATATAAGTAACGTG |
NGT |
2 |
0.0118 |
Tier III |
| 6 |
NC_000011.10 |
11 |
127462775 |
+ |
LOC107984373 |
NNNTGCATATAAGGAAAGTG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
147521872 |
- |
Pan3 |
NNNTGCATATAAGCAACATG |
NGG |
1 |
0.6923 |
Tier I |
| 2 |
NC_000080.6 |
14 |
117005054 |
- |
Gpc6 |
NNNTGAATCTAAGCAACGTG |
NGG |
2 |
0.5306 |
Tier II |
| 3 |
NC_000075.6 |
9 |
114187973 |
+ |
Gm29825 |
NNNTGCAAATAAGCAAAGTG |
NGG |
2 |
0.3733 |
Tier II |
| 4 |
NC_000075.6 |
9 |
114242336 |
- |
4921528I07Rik |
NNNTGCAAATAAGCAAAGTG |
NGG |
2 |
0.3733 |
Tier II |
| 5 |
NC_000076.6 |
10 |
42527020 |
- |
Snx3 |
NNNTGCATATAAGCAATGTT |
NGG |
2 |
0.3267 |
Tier II |
| 6 |
NC_000067.6 |
1 |
119683301 |
+ |
Ptpn4 |
NNNTGGATATAAGCAACCTG |
NGG |
2 |
0.2381 |
Tier II |
| 7 |
NC_000068.7 |
2 |
65894554 |
+ |
Csrnp3 |
NNNTGCCTATAAGCAACCTG |
NGG |
2 |
0.2083 |
Tier II |
| 8 |
NC_000071.6 |
5 |
97074012 |
+ |
Bmp2k |
NNNTTCATATAAGCTACGTG |
NGG |
2 |
0.06 |
Tier II |
| 9 |
NC_000077.6 |
11 |
103020864 |
- |
Dcakd |
NNNTGCATATAAGCAAAGTG |
NGT |
2 |
0.0075 |
Tier II |
| 10 |
NC_000078.6 |
12 |
41017880 |
- |
Gm18954 |
NNNTGCATATAAGCTACTTG |
NGG |
2 |
0.1333 |
Tier III |
Other clones with same target sequence:
(none)
