Construct: sgRNA BRDN0001146137
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCAGATGGTATATGCCGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IGFN1 (91156)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76825
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
201207175 |
+ |
IGFN1 |
NNNAGATGGTATATGCCGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000007.14 |
7 |
128029187 |
+ |
LRRC4 |
NNNAGGTGGTATATGCCGGC |
NGG |
2 |
0.3061 |
Tier I |
| 3 |
NC_000007.14 |
7 |
128029187 |
+ |
SND1 |
NNNAGGTGGTATATGCCGGC |
NGG |
2 |
0.3061 |
Tier II |
| 4 |
NC_000021.9 |
21 |
15825493 |
+ |
USP25 |
NNNAGATGGTATGTGCCGGG |
NGC |
2 |
0.0145 |
Tier II |
| 5 |
NC_000006.12 |
6 |
55552864 |
+ |
HMGCLL1 |
NNNTGATGGTATATGCCGGG |
NGT |
2 |
0.0103 |
Tier II |
| 6 |
NC_000001.11 |
1 |
67326128 |
+ |
IL12RB2 |
NNNAGATGGTATAGCCCGGG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000020.11 |
20 |
15052051 |
- |
MACROD2 |
NNNAGATGGTATAGGCCGGC |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000004.12 |
4 |
142856182 |
+ |
USP38-DT |
NNNAGATGGGATATGCCGGG |
NTG |
2 |
0.0195 |
Tier III |
| 9 |
NC_000004.12 |
4 |
142856182 |
+ |
LOC124900788 |
NNNAGATGGGATATGCCGGG |
NTG |
2 |
0.0195 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
28830164 |
+ |
Lrrc4 |
NNNAGGTGGTATATGCCGGC |
NGG |
2 |
0.3061 |
Tier I |
| 2 |
NC_000072.6 |
6 |
28830164 |
+ |
Snd1 |
NNNAGGTGGTATATGCCGGC |
NGG |
2 |
0.3061 |
Tier II |
| 3 |
NC_000067.6 |
1 |
156820870 |
- |
Ralgps2 |
NNNAGATGGTAGATGCAGGG |
NGG |
2 |
0.2333 |
Tier II |
| 4 |
NC_000067.6 |
1 |
43062089 |
- |
Tgfbrap1 |
NNNAGATGGTATATGCCCGG |
NTG |
2 |
0.0186 |
Tier II |
Other clones with same target sequence:
(none)
