Construct: sgRNA BRDN0001146138
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCATACCTTAAATACGTAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ALK (238)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77393
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
29233576 |
+ |
ALK |
NNNTACCTTAAATACGTAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
61040233 |
+ |
RORA |
NNNTATCTTAAATAGGTAGG |
NGG |
2 |
0.0464 |
Tier II |
| 3 |
NC_000004.12 |
4 |
147846679 |
+ |
ARHGAP10 |
NNNTACCTTAAATCCTTAGG |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000015.10 |
15 |
61040233 |
+ |
LOC107984805 |
NNNTATCTTAAATAGGTAGG |
NGG |
2 |
0.0464 |
Tier III |
| 5 |
NC_000011.10 |
11 |
112533648 |
- |
LINC02763 |
NNNTACCTTAAATACGAAGG |
NGA |
2 |
0.037 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
71905059 |
+ |
Alk |
NNNCACCTTAAACACGTAGG |
NGG |
2 |
0.5108 |
Tier I |
| 2 |
NC_000080.6 |
14 |
30784779 |
- |
Sfmbt1 |
NNNTACCTTAAATAAGTAAG |
NGG |
2 |
0.1587 |
Tier I |
| 3 |
NC_000080.6 |
14 |
101920046 |
- |
Lmo7 |
NNNTACCTCAAATACGTGGG |
NGG |
2 |
0.3692 |
Tier II |
| 4 |
NC_000069.6 |
3 |
29788099 |
+ |
Gm33206 |
NNNTATCTTAAATACATAGG |
NGG |
2 |
0.9286 |
Tier III |
| 5 |
NC_000084.6 |
18 |
28582551 |
- |
4930474G06Rik |
NNNTACCTTAAATAGATAGG |
NGG |
2 |
0.05 |
Tier III |
| 6 |
NC_000076.6 |
10 |
125938510 |
+ |
Gm29944 |
NNNTACCTTAAATACGTACG |
NTG |
2 |
0.0175 |
Tier III |
Other clones with same target sequence:
(none)
