Construct: sgRNA BRDN0001146143
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAGGCAGAATCGCAACTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DGKB (1607)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76918
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
14580923 |
+ |
DGKB |
NNNGGCAGAATCGCAACTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
64212464 |
+ |
MYT1 |
NNNGGCAGAGTGGCAACTGG |
NGG |
2 |
0.2469 |
Tier II |
| 3 |
NC_000016.10 |
16 |
8559748 |
- |
TMEM114 |
NNNGGCAGCATCGCAACTGC |
NGG |
2 |
0.2449 |
Tier II |
| 4 |
NC_000017.11 |
17 |
48529967 |
- |
HOXB1 |
NNNGGCAGAATGGCAAATGG |
NGG |
2 |
0.2074 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
38216048 |
- |
Dgkb |
NNNGGCAGGATGGCAACTGG |
NGG |
2 |
0.2963 |
Tier I |
| 2 |
NC_000078.6 |
12 |
32045624 |
+ |
Prkar2b |
NNNAGCAGAATCACAACTGG |
NGG |
2 |
0.8308 |
Tier II |
| 3 |
NC_000075.6 |
9 |
118679201 |
+ |
Itga9 |
NNNGGCAGAACCGCGACTGG |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000067.6 |
1 |
16943056 |
- |
Jph1 |
NNNGGCAGAATCGCAACTGG |
NAG |
1 |
0.2593 |
Tier II |
| 5 |
NC_000080.6 |
14 |
13352267 |
- |
Synpr |
NNNGGCAGAACCGAAACTGG |
NGG |
2 |
0.2333 |
Tier II |
| 6 |
NC_000068.7 |
2 |
164968700 |
+ |
Slc12a5 |
NNNGGCAGATTCGGAACTGG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
