Construct: sgRNA BRDN0001146144
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGCCAATTAACAGACGATGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAPK6 (5597)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76397
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
52046742 |
+ |
MAPK6 |
NNNCAATTAACAGACGATGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
179151330 |
- |
SESTD1 |
NNNCAATTAACAGAAGATTT |
NGG |
2 |
0.1481 |
Tier I |
| 3 |
NC_000002.12 |
2 |
1262244 |
- |
SNTG2 |
NNNCAATTAACAGACAATGA |
NGG |
2 |
0.5625 |
Tier II |
| 4 |
NC_000008.11 |
8 |
71272023 |
- |
EYA1 |
NNNCAAGTAAAAGACGATGT |
NGG |
2 |
0.4412 |
Tier II |
| 5 |
NC_000005.10 |
5 |
116195939 |
+ |
COMMD10 |
NNNCAATAAACAGAAGATGT |
NGG |
2 |
0.1778 |
Tier II |
| 6 |
NC_000013.11 |
13 |
42070813 |
- |
DGKH |
NNNCAATTAACAGATGATGT |
NGG |
1 |
0.0667 |
Tier II |
| 7 |
NC_000015.10 |
15 |
90873609 |
- |
FURIN |
NNNCATTTAACAGAGGATGT |
NGG |
2 |
0.0357 |
Tier II |
| 8 |
NC_000005.10 |
5 |
132273138 |
- |
PDLIM4 |
NNNCAATTCACAGACGATGT |
NTG |
2 |
0.0223 |
Tier II |
| 9 |
NC_000002.12 |
2 |
1262244 |
- |
LOC124905965 |
NNNCAATTAACAGACAATGA |
NGG |
2 |
0.5625 |
Tier III |
| 10 |
NC_000013.11 |
13 |
42070813 |
- |
MAPK6P3 |
NNNCAATTAACAGATGATGT |
NGG |
1 |
0.0667 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
75397853 |
- |
Mapk6 |
NNNCAGTTAACAGACGATGT |
NGG |
1 |
0.7143 |
Tier I |
| 2 |
NC_000072.6 |
6 |
17665323 |
+ |
Capza2 |
NNNGAATTATCAGACGATGT |
NGG |
2 |
0.4412 |
Tier I |
| 3 |
NC_000067.6 |
1 |
71306875 |
+ |
Abca12 |
NNNTAATTAACAGAAGATGT |
NGG |
2 |
0.1778 |
Tier II |
| 4 |
NC_000077.6 |
11 |
115954469 |
- |
Sap30bp |
NNNCAAATAACAGACTATGT |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000069.6 |
3 |
111995231 |
+ |
Gm6602 |
NNNCAATGGACAGACGATGT |
NGG |
2 |
0.4889 |
Tier III |
| 6 |
NC_000077.6 |
11 |
63961921 |
+ |
Gm30060 |
NNNCAATTAACAGATAATGT |
NGG |
2 |
0.0667 |
Tier III |
Other clones with same target sequence:
(none)
