Construct: sgRNA BRDN0001146145
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATCAGGCCTTGTCCGTGATT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80198
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
97257680 |
+ |
EPHA6 |
NNNAGGCCTTGTCAGTGTTT |
NGG |
2 |
0.175 |
Tier II |
| 2 |
NC_000001.11 |
1 |
175084570 |
- |
TNN |
NNNAGGCCTTGTACCTGATT |
NGG |
2 |
0.1049 |
Tier II |
| 3 |
NC_000012.12 |
12 |
54375348 |
+ |
ZNF385A |
NNNAGGCCTTGTGTGTGATT |
NGG |
2 |
0.1 |
Tier II |
| 4 |
NC_000005.10 |
5 |
170522234 |
- |
KCNIP1 |
NNNAGGCCTTGTCCATGATC |
NGG |
2 |
0.0856 |
Tier II |
| 5 |
NC_000012.12 |
12 |
54375348 |
+ |
GPR84-AS1 |
NNNAGGCCTTGTGTGTGATT |
NGG |
2 |
0.1 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
36534472 |
+ |
Casr |
NNNAGGCTTTGTCTGTGATT |
NGG |
2 |
0.6417 |
Tier II |
| 2 |
NC_000071.6 |
5 |
88930131 |
+ |
Slc4a4 |
NNNAGGCCTGCTCCGTGATT |
NGG |
2 |
0.2143 |
Tier II |
| 3 |
NC_000069.6 |
3 |
24068708 |
- |
Naaladl2 |
NNNAAGCCTTGTCCGTGCTT |
NGG |
2 |
0.1651 |
Tier II |
Other clones with same target sequence:
(none)
