Construct: sgRNA BRDN0001146148
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGTGGCAGAGCCTGATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAK4 (10298)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77574
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
39169681 |
+ |
PAK4 |
NNNGTGGCAGAGCCTGATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
3221848 |
+ |
ITPA |
NNNGTGGCAGGGCCGGATCG |
NGG |
2 |
0.0325 |
Tier I |
| 3 |
NC_000011.10 |
11 |
107336174 |
- |
CWF19L2 |
NNNGTGACAGAGCCTGATCT |
NGG |
2 |
0.7 |
Tier II |
| 4 |
NC_000009.12 |
9 |
97668177 |
- |
NCBP1 |
NNNGTGGCAGAGCCAGAACG |
NGG |
2 |
0.386 |
Tier II |
| 5 |
NC_000009.12 |
9 |
97668177 |
- |
XPA |
NNNGTGGCAGAGCCAGAACG |
NGG |
2 |
0.386 |
Tier II |
| 6 |
NC_000021.9 |
21 |
41504553 |
- |
TMPRSS2 |
NNNGAGGCAGAGCCTGATCT |
NGG |
2 |
0.35 |
Tier II |
| 7 |
NC_000012.12 |
12 |
108152578 |
+ |
WSCD2 |
NNNGTGGCAGAGCCTGCTCT |
NGG |
2 |
0.1235 |
Tier II |
| 8 |
NC_000019.10 |
19 |
10304919 |
- |
ZGLP1 |
NNNGTGGCAGAGCCTGCTAG |
NGG |
2 |
0.0756 |
Tier II |
| 9 |
NC_000016.10 |
16 |
27778408 |
+ |
KATNIP |
NNNGTGGCAGAGCCTGCTCC |
NGG |
2 |
0.0756 |
Tier II |
| 10 |
NC_000011.10 |
11 |
63589613 |
- |
PLAAT3 |
NNNGTGGCATAGCCTGATCG |
NGC |
2 |
0.0181 |
Tier II |
| 11 |
NC_000018.10 |
18 |
8767098 |
+ |
MTCL1 |
NNNGTGGCAGAGGCTGATGG |
NGG |
2 |
0.017 |
Tier II |
| 12 |
NC_000001.11 |
1 |
223360163 |
+ |
SUSD4 |
NNNGTGGCAGAGCCTGATTG |
NGT |
2 |
0.0074 |
Tier II |
| 13 |
NC_000005.10 |
5 |
113909616 |
+ |
LOC124901047 |
NNNGTGGCAGAGCCTGACCC |
NGG |
2 |
0.1837 |
Tier III |
| 14 |
NC_000012.12 |
12 |
127918322 |
+ |
LINC00507 |
NNNGTGGCACATCCTGATCG |
NGG |
2 |
0.1538 |
Tier III |
| 15 |
NC_000012.12 |
12 |
127918322 |
+ |
LINC00508 |
NNNGTGGCACATCCTGATCG |
NGG |
2 |
0.1538 |
Tier III |
| 16 |
NC_000012.12 |
12 |
127918284 |
+ |
LINC00507 |
NNNGTGGCAGATCCCGATCG |
NGG |
2 |
0.1049 |
Tier III |
| 17 |
NC_000012.12 |
12 |
127918284 |
+ |
LINC00508 |
NNNGTGGCAGATCCCGATCG |
NGG |
2 |
0.1049 |
Tier III |
| 18 |
NC_000019.10 |
19 |
10304919 |
- |
FDX2-ZGLP1 |
NNNGTGGCAGAGCCTGCTAG |
NGG |
2 |
0.0756 |
Tier III |
| 19 |
NC_000011.10 |
11 |
63589613 |
- |
LOC105369335 |
NNNGTGGCATAGCCTGATCG |
NGC |
2 |
0.0181 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
28568111 |
- |
Pak4 |
NNNGTGGCAGAGCCTGATTG |
NGG |
1 |
0.4615 |
Tier I |
| 2 |
NC_000073.6 |
7 |
46598105 |
+ |
Sergef |
NNNGTGACATAGCCTGATCG |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000069.6 |
3 |
69375104 |
- |
Ppm1l |
NNNGTGACAGAGCCTGATTG |
NGG |
2 |
0.4615 |
Tier II |
| 4 |
NC_000077.6 |
11 |
26387367 |
+ |
Fancl |
NNNGTGGCAAAGCCTGACCG |
NGG |
2 |
0.4 |
Tier II |
| 5 |
NC_000078.6 |
12 |
76302686 |
+ |
Mthfd1 |
NNNGTGGCAGAGCTTGATTG |
NGG |
2 |
0.3385 |
Tier II |
| 6 |
NC_000069.6 |
3 |
8526778 |
+ |
Stmn2 |
NNNGTGGCAGAGCTTGATCC |
NGG |
2 |
0.3143 |
Tier II |
| 7 |
NC_000075.6 |
9 |
107667574 |
+ |
Slc38a3 |
NNNGTGGGAGAGCCTGATTG |
NGG |
2 |
0.2967 |
Tier II |
| 8 |
NC_000070.6 |
4 |
95051652 |
- |
Jun |
NNNGCGGCAGAGCCCGATCG |
NGG |
2 |
0.2727 |
Tier II |
| 9 |
NC_000067.6 |
1 |
39941367 |
- |
Map4k4 |
NNNGTGGCCCAGCCTGATCG |
NGG |
2 |
0.2286 |
Tier II |
| 10 |
NC_000076.6 |
10 |
98916768 |
+ |
Atp2b1 |
NNNGTGGCAGAGCCGGATTG |
NGG |
2 |
0.0231 |
Tier II |
| 11 |
NC_000076.6 |
10 |
114736305 |
- |
Trhde |
NNNGTGGCAGAGCCTGGTCG |
NCG |
2 |
0.0189 |
Tier II |
| 12 |
NC_000083.6 |
17 |
86596553 |
+ |
Prkce |
NNNGTGGCAGAGCCTGATCC |
NGT |
2 |
0.0069 |
Tier II |
| 13 |
NC_000070.6 |
4 |
131883667 |
+ |
Srsf4 |
NNNGTGGCAGAGCCTGATGG |
NTG |
2 |
0.0049 |
Tier II |
| 14 |
NC_000078.6 |
12 |
87782156 |
- |
Gm21939 |
NNNGTGGCAGAGCCTGACTG |
NGG |
2 |
0.1978 |
Tier III |
Other clones with same target sequence:
(none)
