Construct: sgRNA BRDN0001146151
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTAACTGTGCCTATTCGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FGFR4 (2264)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77571
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
177090800 |
- |
FGFR4 |
NNNAACTGTGCCTATTCGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
171003635 |
+ |
MROH9 |
NNNAACAGTGCCTATTCCAG |
NGG |
2 |
0.4167 |
Tier II |
| 3 |
NC_000014.9 |
14 |
101034013 |
- |
LOC124903407 |
NNNAACTGTGCCTGTCCGAG |
NGG |
2 |
0.3111 |
Tier II |
| 4 |
NC_000005.10 |
5 |
130078848 |
+ |
CHSY3 |
NNNAGCTGTGCCTATTGGAG |
NGG |
2 |
0.0424 |
Tier II |
| 5 |
NC_000007.14 |
7 |
43995218 |
- |
POLR2J4 |
NNNTACTGTGCCTATTCAAG |
NGG |
2 |
0.4406 |
Tier III |
| 6 |
NC_000007.14 |
7 |
102548631 |
- |
POLR2J3-UPK3BL2 |
NNNTACTGTGCCTATTCAAG |
NGG |
2 |
0.4406 |
Tier III |
| 7 |
NC_000007.14 |
7 |
102647725 |
- |
POLR2J2-UPK3BL1 |
NNNTACTGTGCCTATTCAAG |
NGG |
2 |
0.4406 |
Tier III |
| 8 |
NC_000020.11 |
20 |
48557217 |
+ |
LOC105372646 |
NNNAACAGTGCCTATTTGAG |
NGG |
2 |
0.4083 |
Tier III |
| 9 |
NC_000002.12 |
2 |
113980360 |
+ |
LINC01191 |
NNNAACTGTGCATATTCGAG |
NAG |
2 |
0.1852 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
62730671 |
+ |
Arap2 |
NNNAACTGTGCCTATTGAAG |
NGG |
2 |
0.0407 |
Tier I |
| 2 |
NC_000077.6 |
11 |
84144926 |
- |
Acaca |
NNNAACTGTTCCTATTCCAG |
NGG |
2 |
0.3869 |
Tier II |
| 3 |
NC_000080.6 |
14 |
64179163 |
+ |
Msra |
NNNAACTGTTCCTATTCGAG |
NAG |
2 |
0.2106 |
Tier II |
| 4 |
NC_000074.6 |
8 |
62022772 |
- |
Ddx60 |
NNNAACTGTGCCTGGTCGAG |
NGG |
2 |
0.0233 |
Tier II |
| 5 |
NC_000080.6 |
14 |
52428802 |
- |
Tcra |
NNNAACTGTACCCATTCGAG |
NGG |
2 |
0.7368 |
Tier III |
| 6 |
NC_000080.6 |
14 |
52428802 |
- |
Trav1 |
NNNAACTGTACCCATTCGAG |
NGG |
2 |
0.7368 |
Tier III |
| 7 |
NC_000085.6 |
19 |
11090747 |
+ |
Gm336 |
NNNAACTGTGCCTATTGAAG |
NGG |
2 |
0.0407 |
Tier III |
Other clones with same target sequence:
(none)
