Construct: sgRNA BRDN0001146153
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATGACATTCCTTCAACCCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TGFBR3 (7049)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76291
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
91722062 |
- |
TGFBR3 |
NNNGACATTCCTTCAACCCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
76486338 |
- |
ROBO2 |
NNNGACATAACTTCAACCCA |
NGG |
2 |
0.8048 |
Tier II |
| 3 |
NC_000005.10 |
5 |
133334842 |
+ |
FSTL4 |
NNNGGCATCCCTTCAACCCA |
NGG |
2 |
0.6646 |
Tier II |
| 4 |
NC_000012.12 |
12 |
53340917 |
- |
SP7 |
NNNGATGTTCCTTCAACCCA |
NGG |
2 |
0.6555 |
Tier II |
| 5 |
NC_000001.11 |
1 |
97832733 |
- |
DPYD |
NNNGACCTTCCCTCAACCCA |
NGG |
2 |
0.4145 |
Tier II |
| 6 |
NC_000015.10 |
15 |
59134651 |
- |
MYO1E |
NNNGACATCCCTTCAACCAA |
NGG |
2 |
0.3956 |
Tier II |
| 7 |
NC_000009.12 |
9 |
116644529 |
- |
ASTN2 |
NNNGACATGCCTTCAACCCT |
NGG |
2 |
0.3714 |
Tier II |
| 8 |
NC_000002.12 |
2 |
88171437 |
+ |
THNSL2 |
NNNGATATTCCTTCCACCCA |
NGG |
2 |
0.2532 |
Tier II |
| 9 |
NC_000002.12 |
2 |
175985384 |
- |
LNPK |
NNNGACATTGCTTCAATCCA |
NGG |
2 |
0.1815 |
Tier II |
| 10 |
NC_000015.10 |
15 |
98770198 |
- |
IGF1R |
NNNGACATTCATTCAGCCCA |
NGG |
2 |
0.1442 |
Tier II |
| 11 |
NC_000012.12 |
12 |
124793222 |
+ |
SCARB1 |
NNNCACATTCCTGCAACCCA |
NGG |
2 |
0.1381 |
Tier II |
| 12 |
NC_000001.11 |
1 |
111534376 |
- |
TMIGD3 |
NNNGACATTCCTACAACCCA |
NGA |
2 |
0.0481 |
Tier II |
| 13 |
NC_000007.14 |
7 |
138886615 |
- |
KIAA1549 |
NNNGACATTCCTTCTACCCA |
NTG |
2 |
0.0078 |
Tier II |
| 14 |
NC_000001.11 |
1 |
147641419 |
+ |
ACP6 |
NNNGACAGTCCTTCATCCCA |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000015.10 |
15 |
98770198 |
- |
LOC124903560 |
NNNGACATTCATTCAGCCCA |
NGG |
2 |
0.1442 |
Tier III |
| 16 |
NC_000023.11 |
X |
33792200 |
+ |
LOC105373153 |
NNNAACATTCCTTCATCCCA |
NGG |
2 |
0.0 |
Tier III |
| 17 |
NC_000020.11 |
20 |
63081984 |
- |
LINC01749 |
NNNCACATTCCTTCATCCCA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000083.6 |
17 |
26958015 |
+ |
Syngap1 |
NNNGCCATTCCTCCAACCCA |
NGG |
2 |
0.3947 |
Tier II |
| 2 |
NC_000075.6 |
9 |
105880064 |
+ |
Col6a5 |
NNNGACATTCCTTCAACACT |
NGG |
2 |
0.3231 |
Tier II |
| 3 |
NC_000068.7 |
2 |
170415309 |
+ |
Bcas1 |
NNNGACTTTCCTTCAACTCA |
NGG |
2 |
0.2812 |
Tier II |
| 4 |
NC_000073.6 |
7 |
141840158 |
- |
Muc5b |
NNNGACATTTCTGCAACCCA |
NGG |
2 |
0.2455 |
Tier II |
| 5 |
NC_000084.6 |
18 |
21627043 |
+ |
Klhl14 |
NNNGACTTTCTTTCAACCCA |
NGG |
2 |
0.1346 |
Tier II |
| 6 |
NC_000068.7 |
2 |
142232923 |
- |
Macrod2 |
NNNGACATTCCTTTAACCCA |
NCG |
2 |
0.0786 |
Tier II |
| 7 |
NC_000073.6 |
7 |
46214020 |
+ |
Ush1c |
NNNGACATTACTTCAAGCCA |
NGG |
2 |
0.051 |
Tier II |
| 8 |
NC_000070.6 |
4 |
77237200 |
- |
Ptprd |
NNNGACATTCCTTCCACCCA |
NGA |
2 |
0.0189 |
Tier II |
| 9 |
NC_000070.6 |
4 |
86103341 |
- |
Adamtsl1 |
NNNGACATTCTTTCAACCCA |
NTG |
2 |
0.012 |
Tier II |
| 10 |
NC_000068.7 |
2 |
178331084 |
- |
Phactr3 |
NNNGACATTCCTACAACCCA |
NGT |
2 |
0.0112 |
Tier II |
| 11 |
NC_000077.6 |
11 |
103638635 |
- |
Lyzl6 |
NNNGACATTCCTGCATCCCA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000074.6 |
8 |
27476251 |
- |
Poteg |
NNNGACATTCCTTCAACCCA |
NCA |
2 |
0.0 |
Tier II |
| 13 |
NC_000071.6 |
5 |
98553390 |
+ |
Cfap299 |
NNNGACATTCCTTCATCCCA |
NGC |
2 |
0.0 |
Tier II |
| 14 |
NC_000072.6 |
6 |
108652116 |
- |
0610040F04Rik |
NNNGACATGCCATCAACCCA |
NGG |
2 |
0.4952 |
Tier III |
| 15 |
NC_000071.6 |
5 |
28523966 |
+ |
9530036O11Rik |
NNNTACATTCCTCCAACCCA |
NGG |
2 |
0.2871 |
Tier III |
| 16 |
NC_000077.6 |
11 |
25341960 |
+ |
4933427E13Rik |
NNNTACATTCCTTCAACACA |
NGG |
2 |
0.1958 |
Tier III |
| 17 |
NC_000084.6 |
18 |
21627043 |
+ |
Gm31646 |
NNNGACTTTCTTTCAACCCA |
NGG |
2 |
0.1346 |
Tier III |
| 18 |
NC_000068.7 |
2 |
116820061 |
+ |
Gm13990 |
NNNTACATTCCTTCAACCCA |
NGT |
2 |
0.0059 |
Tier III |
Other clones with same target sequence:
(none)
