Construct: sgRNA BRDN0001146154

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGTATTGACTTTGGCCACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AK1 (203)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76579

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 127871906 + AK1 NNNATTGACTTTGGCCACCA NGG 0 1.0 Tier I
2 NC_000005.10 5 134082007 - LOC124901069 NNNATTCACTTTGGCCAACA NGG 2 0.3702 Tier I
3 NC_000001.11 1 162382077 - C1orf226 NNNATTCACTTTGGCCCCCA NGG 2 0.1213 Tier I
4 NC_000002.12 2 166429330 - SCN7A NNNATAAACTTTGGCCACCA NGG 2 0.8667 Tier II
5 NC_000023.11 X 32199370 - DMD NNNAGTGAGTTTGGCCACCA NGG 2 0.3962 Tier II
6 NC_000005.10 5 156421473 + SGCD NNNAATGACTTTGACCACCA NGG 2 0.375 Tier II
7 NC_000005.10 5 134082007 - VDAC1 NNNATTCACTTTGGCCAACA NGG 2 0.3702 Tier II
8 NC_000016.10 16 78313125 - WWOX NNNATTGACTTTGGCCACCC NGG 1 0.2273 Tier II
9 NC_000002.12 2 214204249 - SPAG16 NNNTTTGACTTTGGCTACCA NGG 2 0.1958 Tier II
10 NC_000022.11 22 30195850 + HORMAD2 NNNATTGGCTTTGGCCACCA NAG 2 0.1901 Tier II
11 NC_000002.12 2 191116143 - STAT4 NNNATTGACTTTGTCCATCA NGG 2 0.1714 Tier II
12 NC_000011.10 11 117289131 + BACE1 NNNACTGACTTTGGCCAGCA NGG 2 0.1333 Tier II
13 NC_000001.11 1 197208821 + CRB1 NNNATTGTCTTTGGCTACCA NGG 2 0.1319 Tier II
14 NC_000023.11 X 86693459 + DACH2 NNNATTGACTTTGGCCACAA NAG 2 0.1111 Tier II
15 NC_000002.12 2 42787457 + HAAO NNNATTGACTTTTTCCACCA NGG 2 0.08 Tier II
16 NC_000006.12 6 134984016 + HBS1L NNNATTGACTTTGGCAACCA NGA 2 0.0694 Tier II
17 NC_000005.10 5 55130477 - CDC20B NNNATTGACTGTGGCCTCCA NGG 2 0.0533 Tier II
18 NC_000007.14 7 98957513 + TRRAP NNNATTGACTGTGGCCTCCA NGG 2 0.0533 Tier II
19 NC_000010.11 10 60944958 + RHOBTB1 NNNATTGACTGTGGCCTCCA NGG 2 0.0533 Tier II
20 NC_000007.14 7 67048695 - TYW1 NNNGTTGACTTTGGCCACCA NGA 2 0.0434 Tier II
21 NC_000007.14 7 72778802 + TYW1B NNNGTTGACTTTGGCCACCA NGA 2 0.0434 Tier II
22 NC_000009.12 9 33535205 + ANKRD18B NNNATTGACTTTTGCCACCA NGA 2 0.0208 Tier II
23 NC_000009.12 9 38609581 - ANKRD18A NNNATTGACTTTTGCCACCA NGA 2 0.0208 Tier II
24 NC_000002.12 2 2201220 - MYT1L NNNATTGACTTTGGACACCA NTG 2 0.0087 Tier II
25 NC_000003.12 3 64606920 - ADAMTS9 NNNATTGACTTTGGGGACCA NGG 2 0.0077 Tier II
26 NC_000009.12 9 127871906 + ST6GALNAC4-ST6GALNAC6-AK1 NNNATTGACTTTGGCCACCA NGG 0 1.0 Tier III
27 NC_000016.10 16 52352548 - LOC107984901 NNNACTGTCTTTGGCCACCA NGG 2 0.4286 Tier III
28 NC_000010.11 10 11677106 + LOC105376412 NNNATTGCATTTGGCCACCA NGG 2 0.3673 Tier III
29 NC_000010.11 10 11677106 + LOC105376413 NNNATTGCATTTGGCCACCA NGG 2 0.3673 Tier III
30 NC_000016.10 16 75821006 - LOC105371348 NNNATTCACTTGGGCCACCA NGG 2 0.3438 Tier III
31 NC_000003.12 3 84660825 - LINC00971 NNNATTGGCTTTGGCTACCA NGG 2 0.2256 Tier III
32 NC_000022.11 22 30195850 + LOC105372988 NNNATTGGCTTTGGCCACCA NAG 2 0.1901 Tier III
33 NC_000018.10 18 73688067 - LOC105372190 NNNATTGACTTTGCCCACAA NGG 2 0.1837 Tier III
34 NC_000010.11 10 95123972 - LOC107984257 NNNATTGAGTTTGGCCACCA NAG 2 0.1605 Tier III
35 NC_000005.10 5 100520423 - FAM174A-DT NNNATTGTCTTTGGCTACCA NGG 2 0.1319 Tier III
36 NC_000019.10 19 29734857 + LOC107985345 NNNCTTGACTTTTGCCACCA NGG 2 0.1059 Tier III
37 NC_000010.11 10 60944958 + LOC107984236 NNNATTGACTGTGGCCTCCA NGG 2 0.0533 Tier III
38 NC_000010.11 10 60944958 + LOC124902431 NNNATTGACTGTGGCCTCCA NGG 2 0.0533 Tier III
39 NC_000009.12 9 97194020 - ANKRD18CP NNNATTGACTTTTGCCACCA NGA 2 0.0208 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 89078422 + Slc4a4 NNNACTGACTTTGGCCACCT NGG 2 0.6 Tier II
2 NC_000078.6 12 34744109 + Hdac9 NNNAATGACATTGGCCACCA NGG 2 0.4286 Tier II
3 NC_000083.6 17 78261903 - Crim1 NNNATTGACTTTGCCAACCA NGG 2 0.4286 Tier II
4 NC_000080.6 14 49454615 - Slc35f4 NNNATTGACATTGGCCACTA NGG 2 0.3956 Tier II
5 NC_000073.6 7 109544395 + Denn2b NNNATGGACTCTGGCCACCA NGG 2 0.381 Tier II
6 NC_000074.6 8 83984995 - Prkaca NNNAATGACTCTGGCCACCA NGG 2 0.3333 Tier II
7 NC_000073.6 7 112514807 - Parva NNNATTGACTTTGGCCACAG NGG 2 0.3277 Tier II
8 NC_000073.6 7 63752237 + Otud7a NNNATTGACATTTGCCACCA NGG 2 0.2571 Tier II
9 NC_000071.6 5 124585862 + Gtf2h3 NNNATTTTCTTTGGCCACCA NGG 2 0.2449 Tier II
10 NC_000071.6 5 124586568 + Gtf2h3 NNNATTTTCTTTGGCCACCA NGG 2 0.2449 Tier II
11 NC_000070.6 4 116086792 + Lrrc41 NNNATTGACTTTGGCAGCCA NGG 2 0.1765 Tier II
12 NC_000074.6 8 11798972 + Arhgef7 NNNATTGACTTGGGCTACCA NGG 2 0.1538 Tier II
13 NC_000077.6 11 106839891 - Smurf2 NNNATTGACTGTGGCCACCC NGG 2 0.0909 Tier II
14 NC_000071.6 5 25274395 - Kmt2c NNNATTGACTTTTGCCACCA NAG 2 0.0778 Tier II
15 NC_000082.6 16 41023041 - Lsamp NNNATGGACTTTGGCCACCA NCG 2 0.0612 Tier II
16 NC_000073.6 7 114842565 + Insc NNNATTGCCTTTGGCCTCCA NGG 2 0.0571 Tier II
17 NC_000071.6 5 127786713 - Tmem132d NNNATTGACTTTGGTCACCA NAG 2 0.0173 Tier II
18 NC_000078.6 12 119166984 - Itgb8 NNNATTGGCTTTGGCCACCA NGC 2 0.0163 Tier II
19 NC_000075.6 9 97992477 - Clstn2 NNNATTGACTTTGGCCACCC NTG 2 0.0089 Tier II
20 NC_000080.6 14 59217392 + Rcbtb1 NNNATTGACTTTGGCCACAA NGT 2 0.0069 Tier II
21 NC_000070.6 4 116086792 + Gm52704 NNNATTGACTTTGGCAGCCA NGG 2 0.1765 Tier III
22 NC_000071.6 5 101591685 - Gm29982 NNNAATGACTTTGGCTACCA NGG 2 0.1538 Tier III
23 NC_000076.6 10 15753822 + Gm40604 NNNTTTGACTTTGGCGACCA NGG 2 0.0979 Tier III
24 NC_000080.6 14 79978582 - Gm6999 NNNATTGACTTTTGCCACCA NCG 2 0.0321 Tier III
25 NC_000069.6 3 142664847 - Gm19166 NNNATTGACTGTGGGCACCA NGG 2 0.02 Tier III
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Other clones with same target sequence:

(none)