Construct: sgRNA BRDN0001146157
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCTGGACTTAAGGTCGCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K9 (4293)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77309
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
70800687 |
+ |
MAP3K9 |
NNNTGGACTTAAGGTCGCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
40192694 |
- |
MAP3K10 |
NNNTGGACTTGAGGTCCCGG |
NGG |
2 |
0.1529 |
Tier I |
| 3 |
NC_000017.11 |
17 |
39563970 |
+ |
CDK12 |
NNNTGGACTTGAGGTCGAGG |
NGG |
2 |
0.35 |
Tier II |
| 4 |
NC_000006.12 |
6 |
31750103 |
+ |
MSH5 |
NNNTGGATTTAAGGTCGGGG |
NGG |
2 |
0.1167 |
Tier II |
| 5 |
NC_000015.10 |
15 |
29890144 |
- |
TJP1 |
NNNTGGACTCAAGGTCGGGG |
NGG |
2 |
0.0711 |
Tier II |
| 6 |
NC_000006.12 |
6 |
31750103 |
+ |
MSH5-SAPCD1 |
NNNTGGATTTAAGGTCGGGG |
NGG |
2 |
0.1167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
115254433 |
+ |
Cyp4a29 |
NNNTGTACTTAAGGTCGCTG |
NGG |
2 |
0.4444 |
Tier I |
| 2 |
NC_000078.6 |
12 |
81772700 |
+ |
Map3k9 |
NNNTGGACTTAAGGTCTCGG |
NGG |
1 |
0.25 |
Tier I |
| 3 |
NC_000068.7 |
2 |
158030891 |
- |
Rprd1b |
NNNTGGACTTAAGGTCACTG |
NGG |
2 |
0.6222 |
Tier II |
| 4 |
NC_000070.6 |
4 |
57264791 |
- |
Ptpn3 |
NNNGGGACTTAAGGTGGCGG |
NGG |
2 |
0.0962 |
Tier II |
| 5 |
NC_000076.6 |
10 |
80897036 |
+ |
Tmprss9 |
NNNTGGACTTAATGTCCCGG |
NGG |
2 |
0.0706 |
Tier II |
| 6 |
NC_000077.6 |
11 |
95749094 |
+ |
Zfp652 |
NNNTTGACTTAAGGTGGCGG |
NGG |
2 |
0.0462 |
Tier II |
Other clones with same target sequence:
(none)
