Construct: sgRNA BRDN0001146161
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCTGGGAGCAAAAGACTCGC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- IRAK4 (51135)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75664
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
43771350 |
- |
IRAK4 |
NNNGGGAGCAAAAGACTCGC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
89922433 |
- |
TUBB3 |
NNNGGGAGCAAAGGACTCGC |
NGG |
1 |
0.6522 |
Tier II |
| 3 |
NC_000003.12 |
3 |
27239947 |
- |
NEK10 |
NNNGGGAGAAAAAGACTCTC |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000023.11 |
X |
136179225 |
- |
FHL1 |
NNNGGGAGCAAAAGACCCTC |
NGG |
2 |
0.4706 |
Tier II |
| 5 |
NC_000019.10 |
19 |
16471546 |
+ |
EPS15L1 |
NNNGGGAGACAAAGACTCGC |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000019.10 |
19 |
7930906 |
+ |
TIMM44 |
NNNGGGAGCAAAGGACTGGC |
NGG |
2 |
0.087 |
Tier II |
| 7 |
NC_000002.12 |
2 |
85181622 |
+ |
TCF7L1 |
NNNGGGAGCAAAAGGCTGGC |
NGG |
2 |
0.0867 |
Tier II |
| 8 |
NC_000007.14 |
7 |
32733071 |
- |
MIR550A2 |
NNNGAGAGCAAGAGACTCGC |
NGG |
2 |
0.6259 |
Tier III |
| 9 |
NC_000007.14 |
7 |
32733071 |
- |
MIR550B2 |
NNNGAGAGCAAGAGACTCGC |
NGG |
2 |
0.6259 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
31273271 |
- |
Ncs1 |
NNNGGGAGCAAAAGAATCAC |
NGG |
2 |
0.7143 |
Tier II |
| 2 |
NC_000074.6 |
8 |
117074214 |
+ |
Pkd1l2 |
NNNGGAAGCAAAAGACTCTC |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000081.6 |
15 |
31495025 |
- |
Marchf6 |
NNNGGGAGCAAAAGGCTCTC |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000070.6 |
4 |
58190459 |
+ |
Svep1 |
NNNGGGAGCAAAACACTCAC |
NGG |
2 |
0.3061 |
Tier II |
| 5 |
NC_000068.7 |
2 |
154974218 |
- |
a |
NNNGGGAACAAAATACTCGC |
NGG |
2 |
0.2667 |
Tier II |
| 6 |
NC_000078.6 |
12 |
79845437 |
- |
Rad51b |
NNNGGGAGCTAAAGACTCGT |
NGG |
2 |
0.2647 |
Tier II |
| 7 |
NC_000068.7 |
2 |
5392440 |
- |
Camk1d |
NNNGGGAGCCAAAGACTTGC |
NGG |
2 |
0.2143 |
Tier II |
| 8 |
NC_000074.6 |
8 |
114557519 |
- |
Wwox |
NNNGGGAGCATAGGACTCGC |
NGG |
2 |
0.2007 |
Tier II |
| 9 |
NC_000073.6 |
7 |
141893178 |
+ |
Tollip |
NNNGGGAGCAAAATACTCAC |
NGG |
2 |
0.1905 |
Tier II |
| 10 |
NC_000085.6 |
19 |
23871431 |
- |
Apba1 |
NNNGGGAGCAAAATACTCAC |
NGG |
2 |
0.1905 |
Tier II |
| 11 |
NC_000077.6 |
11 |
6547343 |
- |
Ccm2 |
NNNGGCAGCAAAAGCCTCGC |
NGG |
2 |
0.186 |
Tier II |
| 12 |
NC_000068.7 |
2 |
9874365 |
+ |
Gata3 |
NNNGGGAGCAAAAGATTCCC |
NGG |
2 |
0.1379 |
Tier II |
| 13 |
NC_000082.6 |
16 |
74567773 |
- |
Robo2 |
NNNGGGAGCAAAATACTCCC |
NGG |
2 |
0.1195 |
Tier II |
| 14 |
NC_000081.6 |
15 |
82968260 |
- |
Tcf20 |
NNNGGGAGCATTAGACTCGC |
NGG |
2 |
0.1026 |
Tier II |
| 15 |
NC_000078.6 |
12 |
84569912 |
+ |
Vsx2 |
NNNGGGAGCAAAAGACCCGC |
NGC |
2 |
0.0157 |
Tier II |
| 16 |
NC_000073.6 |
7 |
59662337 |
+ |
Ipw |
NNNGGAAGCAAAAGACTCCC |
NGG |
2 |
0.4483 |
Tier III |
| 17 |
NC_000073.6 |
7 |
59662337 |
+ |
Snhg14 |
NNNGGAAGCAAAAGACTCCC |
NGG |
2 |
0.4483 |
Tier III |
| 18 |
NC_000067.6 |
1 |
45686887 |
+ |
Gm39637 |
NNNGGGAGCAAAATACTCAC |
NGG |
2 |
0.1905 |
Tier III |
Other clones with same target sequence:
(none)
