Construct: sgRNA BRDN0001146164
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GAAAACGACGAGAACCAGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRPF4B (8899)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76428
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
4032730 |
+ |
PRP4K |
NNNAACGACGAGAACCAGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
138296138 |
- |
FAM135B |
NNNAAAGACAAGAACCAGAG |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000013.11 |
13 |
102087505 |
- |
FGF14 |
NNNAATGACGGGAACCAGAG |
NGG |
2 |
0.6036 |
Tier II |
| 4 |
NC_000015.10 |
15 |
79453600 |
- |
MINAR1 |
NNNACCAACGAGAACCAGAG |
NGG |
2 |
0.5 |
Tier II |
| 5 |
NC_000017.11 |
17 |
33999451 |
- |
ASIC2 |
NNNAAAGACGACAACCAGAG |
NGG |
2 |
0.4916 |
Tier II |
| 6 |
NC_000017.11 |
17 |
16702004 |
- |
CCDC144A |
NNNAAGGAAGAGAACCAGAG |
NGG |
2 |
0.4286 |
Tier II |
| 7 |
NC_000001.11 |
1 |
10318230 |
+ |
KIF1B |
NNNAACGACCAGAACCATAG |
NGG |
2 |
0.2667 |
Tier II |
| 8 |
NC_000019.10 |
19 |
57247363 |
- |
ZNF805 |
NNNAACCACGAGCACCAGAG |
NGG |
2 |
0.1447 |
Tier II |
| 9 |
NC_000017.11 |
17 |
18613435 |
+ |
CCDC144BP |
NNNAAGGAAGAGAACCAGAG |
NGG |
2 |
0.4286 |
Tier III |
| 10 |
NC_000017.11 |
17 |
18613435 |
+ |
LOC107985051 |
NNNAAGGAAGAGAACCAGAG |
NGG |
2 |
0.4286 |
Tier III |
| 11 |
NC_000017.11 |
17 |
20333334 |
- |
CCDC144CP |
NNNAAGGAAGAGAACCAGAG |
NGG |
2 |
0.4286 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
34884402 |
+ |
Prpf4b |
NNNAACGGAGAGAACCAGAG |
NGG |
2 |
0.6286 |
Tier I |
| 2 |
NC_000067.6 |
1 |
181923756 |
- |
Enah |
NNNAACGACTAGAACGAGAG |
NGG |
2 |
0.125 |
Tier I |
| 3 |
NC_000068.7 |
2 |
157463042 |
+ |
Src |
NNNAACGCCGAGAACCCGAG |
NGG |
2 |
0.0756 |
Tier I |
| 4 |
NC_000083.6 |
17 |
66811570 |
- |
Ptprm |
NNNAACGAAGAGAACAAGAG |
NGG |
2 |
0.8571 |
Tier II |
| 5 |
NC_000080.6 |
14 |
77052963 |
+ |
Ccdc122 |
NNNAATGATGAGAACCAGAG |
NGG |
2 |
0.8125 |
Tier II |
| 6 |
NC_000078.6 |
12 |
52781720 |
+ |
Akap6 |
NNNAACCACAAGAACCAGAG |
NGG |
2 |
0.6417 |
Tier II |
Other clones with same target sequence:
(none)
