Construct: sgRNA BRDN0001146166
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGCAGTGTTGACCATTACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GUCY2F (2986)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77255
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
109465231 |
- |
GUCY2F |
NNNAGTGTTGACCATTACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
114124859 |
- |
CCDC186 |
NNNAGTGTTGAACATTACAA |
NGG |
2 |
0.6696 |
Tier II |
| 3 |
NC_000022.11 |
22 |
20029906 |
+ |
TANGO2 |
NNNAGTGTTGACCATCACAA |
NGG |
2 |
0.625 |
Tier II |
| 4 |
NC_000001.11 |
1 |
153201264 |
+ |
SPRR2G |
NNNAGTGCTGACCTTTACAG |
NGG |
2 |
0.5333 |
Tier II |
| 5 |
NC_000002.12 |
2 |
229362732 |
+ |
DNER |
NNNAGTGTTGGCCATCACAG |
NGG |
2 |
0.4333 |
Tier II |
| 6 |
NC_000022.11 |
22 |
43422326 |
+ |
MPPED1 |
NNNAGTGGTGAGCATTACAG |
NGG |
2 |
0.3259 |
Tier II |
| 7 |
NC_000003.12 |
3 |
134206800 |
+ |
RYK |
NNNAGTGTTGATCATTACTG |
NGG |
2 |
0.2899 |
Tier II |
| 8 |
NC_000023.11 |
X |
101282723 |
+ |
TAF7L |
NNNAGTGTTTACCACTACAG |
NGG |
2 |
0.2216 |
Tier II |
| 9 |
NC_000010.11 |
10 |
70471439 |
- |
PALD1 |
NNNGGTGTTGACCATGACAG |
NGG |
2 |
0.2163 |
Tier II |
| 10 |
NC_000017.11 |
17 |
9532686 |
- |
STX8 |
NNNAATGTTGACCATTCCAG |
NGG |
2 |
0.1529 |
Tier II |
| 11 |
NC_000017.11 |
17 |
30167877 |
- |
NSRP1 |
NNNACTGTTGACCATTAGAG |
NGG |
2 |
0.1048 |
Tier II |
| 12 |
NC_000012.12 |
12 |
109265895 |
+ |
ACACB |
NNNAGTGTTGACCTTTCCAG |
NGG |
2 |
0.0941 |
Tier II |
| 13 |
NC_000022.11 |
22 |
48751133 |
- |
TAFA5 |
NNNAGTGTTGACCAATACAG |
NGA |
2 |
0.0402 |
Tier II |
| 14 |
NC_000001.11 |
1 |
148991046 |
- |
PDE4DIP |
NNNAGTGTTGCCCAGTACAG |
NGG |
2 |
0.02 |
Tier II |
| 15 |
NC_000002.12 |
2 |
80024808 |
+ |
CTNNA2 |
NNNAGTGTTGACCAGTTCAG |
NGG |
2 |
0.0067 |
Tier II |
| 16 |
NC_000012.12 |
12 |
102304366 |
+ |
LINC02456 |
NNNAGTGTTGGCCATTCCAG |
NGG |
2 |
0.1147 |
Tier III |
| 17 |
NC_000001.11 |
1 |
120531224 |
+ |
PDE4DIPP2 |
NNNAGTGTTGCCCAGTACAG |
NGG |
2 |
0.02 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
142180069 |
- |
Gucy2f |
NNNTGTGTTGACTATTACAG |
NGG |
2 |
0.4455 |
Tier I |
| 2 |
NC_000067.6 |
1 |
150329807 |
+ |
Pdc |
NNNAGTGTTAACCATCACAG |
NGG |
2 |
0.6222 |
Tier II |
| 3 |
NC_000070.6 |
4 |
6370222 |
- |
Sdcbp |
NNNAGTGTTTATCATTACAG |
NGG |
2 |
0.4375 |
Tier II |
| 4 |
NC_000074.6 |
8 |
40900242 |
- |
Slc7a2 |
NNNAGTGTGGTCCATTACAG |
NGG |
2 |
0.1905 |
Tier II |
| 5 |
NC_000072.6 |
6 |
66920990 |
- |
Gng12 |
NNNAGTGTTCACCATGACAG |
NGG |
2 |
0.1385 |
Tier II |
| 6 |
NC_000079.6 |
13 |
9334795 |
+ |
Dip2c |
NNNAGTGTGGACCATTAGAG |
NGG |
2 |
0.0825 |
Tier II |
| 7 |
NC_000077.6 |
11 |
120418108 |
- |
Nploc4 |
NNNAGTGTTGCCCATTCCAG |
NGG |
2 |
0.0706 |
Tier II |
| 8 |
NC_000074.6 |
8 |
61238633 |
+ |
Sh3rf1 |
NNNAGTGTTGACCATCACAG |
NGA |
2 |
0.0463 |
Tier II |
| 9 |
NC_000083.6 |
17 |
78604132 |
+ |
Vit |
NNNAGTGTTGACCATTAGAG |
NGA |
2 |
0.0093 |
Tier II |
| 10 |
NC_000067.6 |
1 |
97875123 |
- |
Pam |
NNNAGTGTTGACCATTGCAG |
NTG |
2 |
0.0069 |
Tier II |
| 11 |
NC_000067.6 |
1 |
150329807 |
+ |
2310030A07Rik |
NNNAGTGTTAACCATCACAG |
NGG |
2 |
0.6222 |
Tier III |
| 12 |
NC_000072.6 |
6 |
66920990 |
- |
4930597O21Rik |
NNNAGTGTTCACCATGACAG |
NGG |
2 |
0.1385 |
Tier III |
| 13 |
NC_000068.7 |
2 |
95493994 |
+ |
Gm13794 |
NNNAGTGTTGACAAGTACAG |
NGG |
2 |
0.0192 |
Tier III |
Other clones with same target sequence:
(none)
