Construct: sgRNA BRDN0001146169

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGACCTCAGGCTAACAGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARK1 (4139)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77795

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 220635887 + MARK1 NNNACCTCAGGCTAACAGTG NGG 0 1.0 Tier I
2 NC_000019.10 19 53601173 - DPRX NNNGTCTCAGGCTAACAGTG NGG 2 0.3977 Tier II
3 NC_000013.11 13 107532550 + NALF1 NNNACCTAAGGCTTACAGTG NGG 2 0.3467 Tier II
4 NC_000012.12 12 41474155 - PDZRN4 NNNACCTCAGCCTAACATTG NGG 2 0.2857 Tier II
5 NC_000010.11 10 95534777 + SORBS1 NNNATCTCAGTCTAACAGTG NGG 2 0.2448 Tier II
6 NC_000006.12 6 107496288 - SOBP NNNCCCTCAGGCTAACATTG NGG 2 0.2353 Tier II
7 NC_000017.11 17 82472721 - NARF NNNACCTCAGGCCAACAGAG NGG 2 0.2256 Tier II
8 NC_000008.11 8 142500646 - ADGRB1 NNNACCTCAGGGTAACACTG NGG 2 0.2116 Tier II
9 NC_000011.10 11 89411596 - NOX4 NNNACCTCAGGCCCACAGTG NGG 2 0.1692 Tier II
10 NC_000012.12 12 10961655 - PRH1 NNNACCTCAGGCCCACAGTG NGG 2 0.1692 Tier II
11 NC_000012.12 12 10961655 - PRH1-TAS2R14 NNNACCTCAGGCCCACAGTG NGG 2 0.1692 Tier II
12 NC_000002.12 2 9281832 - ASAP2 NNNACCTGAGGCTCACAGTG NGG 2 0.1378 Tier II
13 NC_000009.12 9 92504061 + ECM2 NNNAGCTCAGGCTCACAGTG NGG 2 0.1286 Tier II
14 NC_000009.12 9 92504061 + CENPP NNNAGCTCAGGCTCACAGTG NGG 2 0.1286 Tier II
15 NC_000004.12 4 21443690 - KCNIP4 NNNACCTCAGGCTCCCAGTG NGG 2 0.0584 Tier II
16 NC_000005.10 5 144191778 + KCTD16 NNNATCTCAGGCTAACAGTG NGT 2 0.0103 Tier II
17 NC_000017.11 17 21076246 + LINC01563 NNNACCTTATGCTAACAGTG NGG 2 0.7109 Tier III
18 NC_000019.10 19 53601173 - LOC284379 NNNGTCTCAGGCTAACAGTG NGG 2 0.3977 Tier III
19 NC_000013.11 13 107532550 + LOC112268110 NNNACCTAAGGCTTACAGTG NGG 2 0.3467 Tier III
20 NC_000019.10 19 23269825 - IPO5P1 NNNACCTGAGGCTTACAGTG NGG 2 0.3429 Tier III
21 NC_000001.11 1 84629398 + LINC01555 NNNACCTCAGTTTAACAGTG NGG 2 0.2071 Tier III
22 NC_000012.12 12 10961655 - PRH1-PRR4 NNNACCTCAGGCCCACAGTG NGG 2 0.1692 Tier III
23 NC_000012.12 12 31785938 - LOC107984470 NNNACCTCAGGCTATCAATG NGG 2 0.1385 Tier III
24 NC_000002.12 2 241976599 - LINC01237 NNNACCTCAGGCTAACCATG NGG 2 0.1222 Tier III
25 NC_000002.12 2 241976599 - LINC01238 NNNACCTCAGGCTAACCATG NGG 2 0.1222 Tier III
26 NC_000010.11 10 112920721 - LINC02935 NNNACCTCAGGCTCACAGTC NGG 2 0.0918 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 41311773 - Try5 NNNACCTCAGGCTGACTGTG NGG 2 0.0622 Tier I
2 NC_000068.7 2 18947127 + Pip4k2a NNNACCTCATGCTAACAGTG NGG 1 0.8125 Tier II
3 NC_000077.6 11 22957336 - Commd1 NNNACCACAGGCTAGCAGTG NGG 2 0.5688 Tier II
4 NC_000073.6 7 110960767 + Mrvi1 NNNACCTCATGCAAACAGTG NGG 2 0.5625 Tier II
5 NC_000081.6 15 85131559 + Smc1b NNNTCCTCAGGCCAACAGTG NGG 2 0.5024 Tier II
6 NC_000067.6 1 169502780 - Nuf2 NNNAGCTCAGGCCAACAGTG NGG 2 0.4737 Tier II
7 NC_000078.6 12 52800744 - Akap6 NNNGCCTCTGGCTAACAGTG NGG 2 0.375 Tier II
8 NC_000083.6 17 31349958 + Slc37a1 NNNAGCTCAGGCTGACAGTG NGG 2 0.28 Tier II
9 NC_000079.6 13 34647351 + Pxdc1 NNNACCACAGGCTCACAGTG NGG 2 0.1875 Tier II
10 NC_000086.7 X 143737768 + Pak3 NNNACCTCTGGCTCACAGTG NGG 2 0.1286 Tier II
11 NC_000072.6 6 120873131 + Bcl2l13 NNNACCTCAGGCTAACTATG NGG 2 0.0923 Tier II
12 NC_000083.6 17 46930817 - Ubr2 NNNACCTCAGTCTAACTGTG NGG 2 0.0513 Tier II
13 NC_000083.6 17 83753548 - Mta3 NNNGCCTCAGGCTAACAGTG NGA 2 0.0434 Tier II
14 NC_000074.6 8 126304170 + Slc35f3 NNNATCTCAGGCTAACAGTG NTG 2 0.0248 Tier II
15 NC_000069.6 3 88754383 - Syt11 NNNACCTGAGGCTAACAGTG NGC 2 0.0143 Tier II
16 NC_000070.6 4 43767464 - Olfr269-ps1 NNNACCTCAGGACAACAGTG NGG 2 0.5639 Tier III
17 NC_000076.6 10 92091829 + Rmst NNNACCTCAAGCTAACACTG NGG 2 0.4444 Tier III
18 NC_000078.6 12 52800744 - Gm51971 NNNGCCTCTGGCTAACAGTG NGG 2 0.375 Tier III
19 NC_000078.6 12 94004737 - Gm7119 NNNTCCTCAGGCTAACACTG NGG 2 0.303 Tier III
20 NC_000072.6 6 41311773 - Tcrb NNNACCTCAGGCTGACTGTG NGG 2 0.0622 Tier III
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Other clones with same target sequence:

(none)