Construct: sgRNA BRDN0001146170
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCTTCCGAAGATCTTCTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME1 (4830)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75538
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
51159996 |
+ |
NME1 |
NNNTTCCGAAGATCTTCTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
51159996 |
+ |
NME1-NME2 |
NNNTTCCGAAGATCTTCTCA |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000012.12 |
12 |
128391080 |
- |
TMEM132C |
NNNTTCCAAAGAACTTCTCA |
NGG |
2 |
0.6923 |
Tier II |
| 4 |
NC_000010.11 |
10 |
16792085 |
+ |
RSU1 |
NNNTTTCTAAGATCTTCTCA |
NGG |
2 |
0.5804 |
Tier II |
| 5 |
NC_000015.10 |
15 |
61155579 |
- |
RORA |
NNNTTCCAAAGATCTTCCCA |
NGG |
2 |
0.4286 |
Tier II |
| 6 |
NC_000010.11 |
10 |
113155089 |
+ |
TCF7L2 |
NNNTTCCTGAGATCTTCTCA |
NGG |
2 |
0.4167 |
Tier II |
| 7 |
NC_000018.10 |
18 |
36662554 |
- |
FHOD3 |
NNNTTCCCAAGATCTTCTAA |
NGG |
2 |
0.2637 |
Tier II |
| 8 |
NC_000002.12 |
2 |
64118805 |
- |
PELI1 |
NNNTTCCCAAGATCTTCTCA |
NGA |
2 |
0.0427 |
Tier II |
| 9 |
NC_000014.9 |
14 |
56326408 |
+ |
LINC02284 |
NNNTTCCTAAGATCTTATCA |
NGG |
2 |
0.2917 |
Tier III |
| 10 |
NC_000018.10 |
18 |
36662554 |
- |
LOC105372071 |
NNNTTCCCAAGATCTTCTAA |
NGG |
2 |
0.2637 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
24547812 |
+ |
Xrcc1 |
NNNTGCCGAAAATCTTCTCA |
NGG |
2 |
0.64 |
Tier I |
| 2 |
NC_000085.6 |
19 |
58347754 |
+ |
Gfra1 |
NNNTTCCCAAGATCTTCACA |
NGG |
2 |
0.4103 |
Tier II |
| 3 |
NC_000071.6 |
5 |
123038916 |
- |
Morn3 |
NNNTTCCGAGGAACTTCTCA |
NGG |
2 |
0.3846 |
Tier II |
| 4 |
NC_000078.6 |
12 |
41233248 |
+ |
Immp2l |
NNNCTCCGAAGAGCTTCTCA |
NGG |
2 |
0.1688 |
Tier II |
| 5 |
NC_000067.6 |
1 |
156645982 |
+ |
Abl2 |
NNNTTCCCAAGATCTTCTCC |
NGG |
2 |
0.1399 |
Tier II |
| 6 |
NC_000085.6 |
19 |
42776355 |
- |
Hps1 |
NNNTTCCGCAGATCTTCTCC |
NGG |
2 |
0.1299 |
Tier II |
| 7 |
NC_000081.6 |
15 |
103254020 |
+ |
Nfe2 |
NNNTTCCAAAGATCTTCTCA |
NGA |
2 |
0.0694 |
Tier II |
| 8 |
NC_000071.6 |
5 |
123038916 |
- |
Gm42023 |
NNNTTCCGAGGAACTTCTCA |
NGG |
2 |
0.3846 |
Tier III |
| 9 |
NC_000085.6 |
19 |
36234457 |
+ |
Gm32027 |
NNNTTCCTAAGATCTTCTCA |
NAG |
2 |
0.162 |
Tier III |
Other clones with same target sequence:
(none)
