Construct: sgRNA BRDN0001146173
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAATCTTGTGAACCTCATCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDKL4 (344387)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76106
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
39225930 |
- |
CDKL4 |
NNNTCTTGTGAACCTCATCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
135796264 |
- |
SLC25A48 |
NNNTATTGTGAACCACATCG |
NGG |
2 |
0.3308 |
Tier II |
| 3 |
NC_000003.12 |
3 |
156342870 |
- |
KCNAB1 |
NNNTCTTGTGCACCTCAGCG |
NGG |
2 |
0.1333 |
Tier II |
| 4 |
NC_000016.10 |
16 |
25959473 |
+ |
HS3ST4 |
NNNTCTTGTGAACCCCATAG |
NGG |
2 |
0.1169 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
109592232 |
- |
Rtl1 |
NNNCCTTATGAACCTCATCG |
NGG |
2 |
0.6471 |
Tier I |
| 2 |
NC_000083.6 |
17 |
80556398 |
- |
Cdkl4 |
NNNCCTCGTGAACCTCATCG |
NGG |
2 |
0.4449 |
Tier I |
| 3 |
NC_000074.6 |
8 |
15085036 |
+ |
Myom2 |
NNNTCATGGGAACCTCATCG |
NGG |
2 |
0.5365 |
Tier II |
| 4 |
NC_000074.6 |
8 |
10303455 |
+ |
Myo16 |
NNNTGTTGTGAACCTCACCG |
NGG |
2 |
0.2571 |
Tier II |
| 5 |
NC_000071.6 |
5 |
44693479 |
+ |
Ldb2 |
NNNTCTTGTGACCCTCATCT |
NGG |
2 |
0.1842 |
Tier II |
| 6 |
NC_000078.6 |
12 |
109592232 |
- |
6430411K18Rik |
NNNCCTTATGAACCTCATCG |
NGG |
2 |
0.6471 |
Tier III |
Other clones with same target sequence:
(none)
