Construct: sgRNA BRDN0001146174

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTCATCAGCCGTGTCACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUAK2 (81788)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77002

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 205304171 + NUAK2 NNNCATCAGCCGTGTCACTG NGG 0 1.0 Tier I
2 NC_000021.9 21 41992757 - ZBTB21 NNNCATCATCCGCGTCACTG NGG 2 0.4211 Tier I
3 NC_000019.10 19 7605298 - CAMSAP3 NNNCAGCAGCCGTGTCACCG NGG 2 0.1576 Tier I
4 NC_000003.12 3 69903611 + MITF NNNCAACAGCCTTGTCACTG NGG 2 0.3333 Tier II
5 NC_000002.12 2 26616063 + CIB4 NNNCATCAGCCATGTGACTG NGG 2 0.1436 Tier II
6 NC_000012.12 12 108884778 + DAO NNNCACCAGCCGTGTGACTG NGG 2 0.1399 Tier II
7 NC_000019.10 19 56142759 - ZNF444 NNNCATCAGCAGTGTGACTG NGG 2 0.1154 Tier II
8 NC_000022.11 22 28003292 - TTC28 NNNCATCAGCTGTGCCACTG NGG 2 0.0839 Tier II
9 NC_000022.11 22 19957763 + COMT NNNCATCAGCCGTTTCACAG NGG 2 0.0762 Tier II
10 NC_000023.11 X 124414289 - TENM1 NNNCATCAGTCGTGTCACTG NTG 2 0.0367 Tier II
11 NC_000002.12 2 50705763 - NRXN1 NNNCATCAGCGGTGTCTCTG NGG 2 0.0333 Tier II
12 NC_000004.12 4 3040547 + GRK4 NNNCATCAGCCGTGTGCCTG NGG 2 0.0271 Tier II
13 NC_000004.12 4 98547007 - TSPAN5 NNNCATCAGCCGTGTGCCTG NGG 2 0.0271 Tier II
14 NC_000002.12 2 207613994 - METTL21A NNNCATCAGCCGTGTCACTG NTT 2 0.0 Tier II
15 NC_000018.10 18 52110570 + LOC105372121 NNNCAGCAGCCGTGTAACTG NGG 2 0.5714 Tier III
16 NC_000003.12 3 127360739 - LINC02016 NNNCCTCAGCAGTGTCACTG NGG 2 0.375 Tier III
17 NC_000002.12 2 58848721 - LINC01122 NNNCATCAGCTGTATCACTG NGG 2 0.2308 Tier III
18 NC_000008.11 8 11580307 + LINC00208 NNNCACCAGCCGTGTGACTG NGG 2 0.1399 Tier III
19 NC_000010.11 10 9460091 - LINC02663 NNNCATGAGCCGTGTCACTG NGC 2 0.0105 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000074.6 8 3597914 - Camsap3 NNNCAGCAGCCGCGTCACTG NGG 2 0.4511 Tier I
2 NC_000082.6 16 97951743 - Zbtb21 NNNCATCATCCGGGTCACTG NGG 2 0.1391 Tier I
3 NC_000070.6 4 107073929 + Cyb5rl NNNCATCAGCCCTGTCACTG NAG 2 0.1373 Tier I
4 NC_000067.6 1 160984419 - Serpinc1 NNNCAACAGCCCTGTCACTG NGG 2 0.4588 Tier II
5 NC_000068.7 2 179546758 - Cdh4 NNNCAGCAGCAGTGTCACTG NGG 2 0.4286 Tier II
6 NC_000079.6 13 55000997 - Unc5a NNNCATTAGCCTTGTCACTG NGG 2 0.3125 Tier II
7 NC_000071.6 5 151174164 - Stard13 NNNCATCAGCAGTGTTACTG NGG 2 0.2308 Tier II
8 NC_000070.6 4 137699922 + Rap1gap NNNCATCAGCCGTCTCACTC NGG 2 0.1837 Tier II
9 NC_000070.6 4 108916099 - Rab3b NNNCATCAGCCGTGTGATTG NGG 2 0.0989 Tier II
10 NC_000074.6 8 56320737 - Hpgd NNNCATCAGACGTGTCACTG NGA 2 0.0602 Tier II
11 NC_000081.6 15 41625368 + Oxr1 NNNCATCAGCCGTCTCACTG NGA 2 0.0298 Tier II
12 NC_000074.6 8 18852901 - Agpat5 NNNCATCAGCTGTGGCACTG NGG 2 0.0154 Tier II
13 NC_000078.6 12 5629968 + Gm32398 NNNCATCAGAAGTGTCACTG NGG 2 0.65 Tier III
14 NC_000070.6 4 137699922 + Rap1gapos NNNCATCAGCCGTCTCACTC NGG 2 0.1837 Tier III
15 NC_000069.6 3 85056128 - Gm30991 NNNCATCAGCCGTGTCACTT NAG 2 0.1815 Tier III
16 NC_000069.6 3 41480997 + Gm40040 NNNCATCAGCCTTGTCTCTG NGG 2 0.0513 Tier III
Download CSV

Other clones with same target sequence:

(none)