Construct: sgRNA BRDN0001146174
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTCATCAGCCGTGTCACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NUAK2 (81788)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77002
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000001.11 |
1 |
205304171 |
+ |
NUAK2 |
NNNCATCAGCCGTGTCACTG |
NGG |
0 |
1.0 |
Tier I |
2 |
NC_000021.9 |
21 |
41992757 |
- |
ZBTB21 |
NNNCATCATCCGCGTCACTG |
NGG |
2 |
0.4211 |
Tier I |
3 |
NC_000019.10 |
19 |
7605298 |
- |
CAMSAP3 |
NNNCAGCAGCCGTGTCACCG |
NGG |
2 |
0.1576 |
Tier I |
4 |
NC_000003.12 |
3 |
69903611 |
+ |
MITF |
NNNCAACAGCCTTGTCACTG |
NGG |
2 |
0.3333 |
Tier II |
5 |
NC_000002.12 |
2 |
26616063 |
+ |
CIB4 |
NNNCATCAGCCATGTGACTG |
NGG |
2 |
0.1436 |
Tier II |
6 |
NC_000012.12 |
12 |
108884778 |
+ |
DAO |
NNNCACCAGCCGTGTGACTG |
NGG |
2 |
0.1399 |
Tier II |
7 |
NC_000019.10 |
19 |
56142759 |
- |
ZNF444 |
NNNCATCAGCAGTGTGACTG |
NGG |
2 |
0.1154 |
Tier II |
8 |
NC_000022.11 |
22 |
28003292 |
- |
TTC28 |
NNNCATCAGCTGTGCCACTG |
NGG |
2 |
0.0839 |
Tier II |
9 |
NC_000022.11 |
22 |
19957763 |
+ |
COMT |
NNNCATCAGCCGTTTCACAG |
NGG |
2 |
0.0762 |
Tier II |
10 |
NC_000023.11 |
X |
124414289 |
- |
TENM1 |
NNNCATCAGTCGTGTCACTG |
NTG |
2 |
0.0367 |
Tier II |
11 |
NC_000002.12 |
2 |
50705763 |
- |
NRXN1 |
NNNCATCAGCGGTGTCTCTG |
NGG |
2 |
0.0333 |
Tier II |
12 |
NC_000004.12 |
4 |
3040547 |
+ |
GRK4 |
NNNCATCAGCCGTGTGCCTG |
NGG |
2 |
0.0271 |
Tier II |
13 |
NC_000004.12 |
4 |
98547007 |
- |
TSPAN5 |
NNNCATCAGCCGTGTGCCTG |
NGG |
2 |
0.0271 |
Tier II |
14 |
NC_000002.12 |
2 |
207613994 |
- |
METTL21A |
NNNCATCAGCCGTGTCACTG |
NTT |
2 |
0.0 |
Tier II |
15 |
NC_000018.10 |
18 |
52110570 |
+ |
LOC105372121 |
NNNCAGCAGCCGTGTAACTG |
NGG |
2 |
0.5714 |
Tier III |
16 |
NC_000003.12 |
3 |
127360739 |
- |
LINC02016 |
NNNCCTCAGCAGTGTCACTG |
NGG |
2 |
0.375 |
Tier III |
17 |
NC_000002.12 |
2 |
58848721 |
- |
LINC01122 |
NNNCATCAGCTGTATCACTG |
NGG |
2 |
0.2308 |
Tier III |
18 |
NC_000008.11 |
8 |
11580307 |
+ |
LINC00208 |
NNNCACCAGCCGTGTGACTG |
NGG |
2 |
0.1399 |
Tier III |
19 |
NC_000010.11 |
10 |
9460091 |
- |
LINC02663 |
NNNCATGAGCCGTGTCACTG |
NGC |
2 |
0.0105 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
1 |
NC_000074.6 |
8 |
3597914 |
- |
Camsap3 |
NNNCAGCAGCCGCGTCACTG |
NGG |
2 |
0.4511 |
Tier I |
2 |
NC_000082.6 |
16 |
97951743 |
- |
Zbtb21 |
NNNCATCATCCGGGTCACTG |
NGG |
2 |
0.1391 |
Tier I |
3 |
NC_000070.6 |
4 |
107073929 |
+ |
Cyb5rl |
NNNCATCAGCCCTGTCACTG |
NAG |
2 |
0.1373 |
Tier I |
4 |
NC_000067.6 |
1 |
160984419 |
- |
Serpinc1 |
NNNCAACAGCCCTGTCACTG |
NGG |
2 |
0.4588 |
Tier II |
5 |
NC_000068.7 |
2 |
179546758 |
- |
Cdh4 |
NNNCAGCAGCAGTGTCACTG |
NGG |
2 |
0.4286 |
Tier II |
6 |
NC_000079.6 |
13 |
55000997 |
- |
Unc5a |
NNNCATTAGCCTTGTCACTG |
NGG |
2 |
0.3125 |
Tier II |
7 |
NC_000071.6 |
5 |
151174164 |
- |
Stard13 |
NNNCATCAGCAGTGTTACTG |
NGG |
2 |
0.2308 |
Tier II |
8 |
NC_000070.6 |
4 |
137699922 |
+ |
Rap1gap |
NNNCATCAGCCGTCTCACTC |
NGG |
2 |
0.1837 |
Tier II |
9 |
NC_000070.6 |
4 |
108916099 |
- |
Rab3b |
NNNCATCAGCCGTGTGATTG |
NGG |
2 |
0.0989 |
Tier II |
10 |
NC_000074.6 |
8 |
56320737 |
- |
Hpgd |
NNNCATCAGACGTGTCACTG |
NGA |
2 |
0.0602 |
Tier II |
11 |
NC_000081.6 |
15 |
41625368 |
+ |
Oxr1 |
NNNCATCAGCCGTCTCACTG |
NGA |
2 |
0.0298 |
Tier II |
12 |
NC_000074.6 |
8 |
18852901 |
- |
Agpat5 |
NNNCATCAGCTGTGGCACTG |
NGG |
2 |
0.0154 |
Tier II |
13 |
NC_000078.6 |
12 |
5629968 |
+ |
Gm32398 |
NNNCATCAGAAGTGTCACTG |
NGG |
2 |
0.65 |
Tier III |
14 |
NC_000070.6 |
4 |
137699922 |
+ |
Rap1gapos |
NNNCATCAGCCGTCTCACTC |
NGG |
2 |
0.1837 |
Tier III |
15 |
NC_000069.6 |
3 |
85056128 |
- |
Gm30991 |
NNNCATCAGCCGTGTCACTT |
NAG |
2 |
0.1815 |
Tier III |
16 |
NC_000069.6 |
3 |
41480997 |
+ |
Gm40040 |
NNNCATCAGCCTTGTCTCTG |
NGG |
2 |
0.0513 |
Tier III |
Other clones with same target sequence:
(none)