Construct: sgRNA BRDN0001146176
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCATGGTGTTGGTCGACATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PAN3 (255967)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77426
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
28267136 |
+ |
PAN3 |
NNNTGGTGTTGGTCGACATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
40083952 |
- |
SLC2A13 |
NNNTGGTGTTGATCGAAATG |
NGG |
2 |
0.4356 |
Tier II |
| 3 |
NC_000001.11 |
1 |
243710722 |
- |
AKT3 |
NNNTGGCTTTGGTCGACATG |
NGG |
2 |
0.4297 |
Tier II |
| 4 |
NC_000023.11 |
X |
112636418 |
- |
LHFPL1 |
NNNTGGTGTTTGTCTACATG |
NGG |
2 |
0.0549 |
Tier II |
| 5 |
NC_000001.11 |
1 |
180663907 |
- |
XPR1 |
NNNTAGTGTTGGTGGACATG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000003.12 |
3 |
149688003 |
+ |
WWTR1 |
NNNTGGTGGTGGTGGACATG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000018.10 |
18 |
57714179 |
+ |
ATP8B1 |
NNNTGGTGGTGGTGGACATG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000020.11 |
20 |
20137520 |
- |
CFAP61 |
NNNTGGTGGTGGTGGACATG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000011.10 |
11 |
44243039 |
+ |
EXT2 |
NNNTGGTGTTGGTGGACATG |
NTG |
2 |
0.0 |
Tier II |
| 10 |
NC_000012.12 |
12 |
40083952 |
- |
LOC112268095 |
NNNTGGTGTTGATCGAAATG |
NGG |
2 |
0.4356 |
Tier III |
| 11 |
NC_000006.12 |
6 |
131111317 |
+ |
LOC102723445 |
NNNTTGTGTTGGTTGACATG |
NGG |
2 |
0.22 |
Tier III |
| 12 |
NC_000001.11 |
1 |
224795450 |
- |
LOC105373108 |
NNNTGGTGGTGGTGGACATG |
NGG |
2 |
0.0 |
Tier III |
| 13 |
NC_000017.11 |
17 |
3172784 |
+ |
LOC100288728 |
NNNTGGTGGTGGTGGACATG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
147526966 |
+ |
Pan3 |
NNNTGGTGTTGGTTGATATG |
NGG |
2 |
0.3422 |
Tier I |
| 2 |
NC_000068.7 |
2 |
60213269 |
+ |
Marchf7 |
NNNTGGTGTTGGTTGACATT |
NGG |
2 |
0.5133 |
Tier II |
| 3 |
NC_000067.6 |
1 |
129543345 |
+ |
Thsd7b |
NNNTGGTGTGGGTTGACATG |
NGG |
2 |
0.3667 |
Tier II |
| 4 |
NC_000073.6 |
7 |
30482450 |
- |
Nphs1 |
NNNTGGAGTTGGTAGACATG |
NGG |
2 |
0.3062 |
Tier II |
| 5 |
NC_000078.6 |
12 |
109572444 |
- |
Rtl1 |
NNNTGGTCTTGGTAGACATG |
NGG |
2 |
0.2154 |
Tier II |
| 6 |
NC_000068.7 |
2 |
70254322 |
+ |
Myo3b |
NNNAGGTGTTGGTAGACATG |
NGG |
2 |
0.1667 |
Tier II |
| 7 |
NC_000071.6 |
5 |
105444559 |
+ |
Lrrc8b |
NNNTGTTGTTGGTCGGCATG |
NGG |
2 |
0.1282 |
Tier II |
| 8 |
NC_000079.6 |
13 |
11570019 |
+ |
Ryr2 |
NNNTGGTGTTGGTTGACATG |
NGA |
2 |
0.0509 |
Tier II |
| 9 |
NC_000081.6 |
15 |
93456142 |
+ |
Pphln1 |
NNNTGGTGTTGGTCTACAGG |
NGG |
2 |
0.0357 |
Tier II |
| 10 |
NC_000072.6 |
6 |
134651421 |
+ |
Borcs5 |
NNNTGGTCTTGGTGGACATG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000073.6 |
7 |
49566708 |
+ |
Nav2 |
NNNTGGTGTTGGTGGACTTG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
