Construct: sgRNA BRDN0001146177
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TACCTCCAGAATCAGTGGAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME6 (10201)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76223
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
48298441 |
+ |
NME6 |
NNNCTCCAGAATCAGTGGAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
183446461 |
- |
CDKN2AIP |
NNNGTCTAGAATCAGTGGAT |
NGG |
2 |
0.4063 |
Tier I |
| 3 |
NC_000003.12 |
3 |
184368157 |
+ |
POLR2H |
NNNCTCCAGAATCACGGGAT |
NGG |
2 |
0.0944 |
Tier I |
| 4 |
NC_000008.11 |
8 |
115604767 |
+ |
TRPS1 |
NNNCTCCAGAATCACTGGAT |
NGA |
2 |
0.0189 |
Tier I |
| 5 |
NC_000001.11 |
1 |
165558582 |
+ |
LRRC52 |
NNNCTCCAGGATCAGTAGAT |
NGG |
2 |
0.5185 |
Tier II |
| 6 |
NC_000003.12 |
3 |
38647953 |
- |
SCN5A |
NNNCTAGAGAATCAGTGGAT |
NGG |
2 |
0.437 |
Tier II |
| 7 |
NC_000002.12 |
2 |
230060836 |
- |
SLC16A14 |
NNNCTCCAGACTCAGAGGAT |
NGG |
2 |
0.3636 |
Tier II |
| 8 |
NC_000009.12 |
9 |
127951459 |
+ |
EEIG1 |
NNNCTCCAGAATCAGGGAAT |
NGG |
2 |
0.2396 |
Tier II |
| 9 |
NC_000002.12 |
2 |
134667004 |
+ |
TMEM163 |
NNNTTCCAGAATCAGTGGAT |
NAG |
2 |
0.2074 |
Tier II |
| 10 |
NC_000018.10 |
18 |
62128539 |
+ |
PIGN |
NNNCTCCAGAATCACTGCAT |
NGG |
2 |
0.1299 |
Tier II |
| 11 |
NC_000016.10 |
16 |
84395055 |
+ |
ATP2C2 |
NNNCTCCATAATCCGTGGAT |
NGG |
2 |
0.1143 |
Tier II |
| 12 |
NC_000007.14 |
7 |
137655920 |
- |
DGKI |
NNNCTCCAGAATCAGTGGAT |
NCG |
1 |
0.1071 |
Tier II |
| 13 |
NC_000003.12 |
3 |
7271623 |
+ |
GRM7 |
NNNCTCCAGACTCAGTGGAT |
NAG |
2 |
0.1037 |
Tier II |
| 14 |
NC_000023.11 |
X |
81120233 |
- |
HMGN5 |
NNNGTCCAGAATCAGTGGAG |
NGG |
2 |
0.0882 |
Tier II |
| 15 |
NC_000007.14 |
7 |
91209837 |
- |
CDK14 |
NNNCCCCAGAATCAGTGGAT |
NGA |
2 |
0.0694 |
Tier II |
| 16 |
NC_000012.12 |
12 |
44804388 |
+ |
NELL2 |
NNNCTCCAGAATCAGTGGAA |
NTG |
2 |
0.0219 |
Tier II |
| 17 |
NC_000010.11 |
10 |
50547229 |
+ |
SGMS1 |
NNNCTCCAGAATCAGTGGTT |
NTG |
2 |
0.021 |
Tier II |
| 18 |
NC_000001.11 |
1 |
165558582 |
+ |
LRRC52-AS1 |
NNNCTCCAGGATCAGTAGAT |
NGG |
2 |
0.5185 |
Tier III |
| 19 |
NC_000003.12 |
3 |
34673918 |
+ |
LOC124909365 |
NNNCTCCAGAAACAGGGGAT |
NGG |
2 |
0.2769 |
Tier III |
| 20 |
NC_000007.14 |
7 |
118465154 |
+ |
LOC105375472 |
NNNCACCAGAATCAGGGGAT |
NGG |
2 |
0.1731 |
Tier III |
| 21 |
NC_000005.10 |
5 |
88982510 |
- |
MEF2C-AS1 |
NNNCTCCAGAAGCAGTGGAT |
NGT |
2 |
0.0081 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
109835401 |
- |
Nme6 |
NNNCTCCAGGATCAGTGGGT |
NGG |
2 |
0.2083 |
Tier I |
| 2 |
NC_000072.6 |
6 |
91243884 |
+ |
Fbln2 |
NNNCTGCAGAGTCAGTGGAT |
NGG |
2 |
0.325 |
Tier II |
| 3 |
NC_000070.6 |
4 |
91365455 |
- |
Elavl2 |
NNNCTCCAGGATCAGTGGGT |
NGG |
2 |
0.2083 |
Tier II |
| 4 |
NC_000075.6 |
9 |
45620729 |
- |
Dscaml1 |
NNNCCCCAGAATCAGTGGCT |
NGG |
2 |
0.2069 |
Tier II |
| 5 |
NC_000076.6 |
10 |
10427150 |
- |
Adgb |
NNNCTCCAGATTCAGTGGAA |
NGG |
2 |
0.1731 |
Tier II |
| 6 |
NC_000068.7 |
2 |
73115021 |
+ |
Ola1 |
NNNCTCCAGTATCAGTGGAG |
NGG |
2 |
0.1557 |
Tier II |
| 7 |
NC_000081.6 |
15 |
95061839 |
+ |
Tmem117 |
NNNCTCCAGAATCAGTGGAA |
NAG |
2 |
0.1458 |
Tier II |
| 8 |
NC_000080.6 |
14 |
118581273 |
+ |
Abcc4 |
NNNCTCCAGCATCAGTGGGT |
NGG |
2 |
0.125 |
Tier II |
| 9 |
NC_000071.6 |
5 |
93109387 |
- |
Septin11 |
NNNCTCCAGCATCAGGGGAT |
NGG |
2 |
0.1154 |
Tier II |
| 10 |
NC_000069.6 |
3 |
54162520 |
+ |
Trpc4 |
NNNCTTCAGAATCAGTGGAC |
NGG |
2 |
0.0844 |
Tier II |
| 11 |
NC_000068.7 |
2 |
102353981 |
- |
Trim44 |
NNNCTCCAGGATCAGTGGAT |
NGA |
2 |
0.0386 |
Tier II |
| 12 |
NC_000068.7 |
2 |
141876683 |
+ |
Macrod2 |
NNNCTCCAGAATCGGTGGAT |
NTG |
2 |
0.0182 |
Tier II |
| 13 |
NC_000075.6 |
9 |
119433496 |
+ |
Acvr2b |
NNNCTCCAGACTCAGTGGAT |
NTG |
2 |
0.0156 |
Tier II |
| 14 |
NC_000067.6 |
1 |
161550227 |
+ |
Gm31815 |
NNNCTCCAAAATCAGTGCAT |
NGG |
2 |
0.3061 |
Tier III |
| 15 |
NC_000079.6 |
13 |
117065275 |
- |
Gm29767 |
NNNCTCCAGAGTCAGGGGAT |
NGG |
2 |
0.225 |
Tier III |
| 16 |
NC_000070.6 |
4 |
91365455 |
- |
Gm12653 |
NNNCTCCAGGATCAGTGGGT |
NGG |
2 |
0.2083 |
Tier III |
| 17 |
NC_000074.6 |
8 |
102296861 |
+ |
Gm39233 |
NNNCTCCAGGATCAGTGGAT |
NGA |
2 |
0.0386 |
Tier III |
Other clones with same target sequence:
(none)
