Construct: sgRNA BRDN0001146179
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGAGATTATTGGCAAGGGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ACVR1B (91)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76681
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
51981034 |
+ |
ACVR1B |
NNNAGATTATTGGCAAGGGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
73775576 |
+ |
FUT11 |
NNNAGATTATTGGCAAGGTC |
NGG |
2 |
0.0606 |
Tier I |
| 3 |
NC_000008.11 |
8 |
117987379 |
+ |
EXT1 |
NNNAGATCAATGGCAAGGGT |
NGG |
2 |
0.8571 |
Tier II |
| 4 |
NC_000008.11 |
8 |
98539122 |
- |
STK3 |
NNNAAATAATTGGCAAGGGT |
NGG |
2 |
0.6933 |
Tier II |
| 5 |
NC_000016.10 |
16 |
67440564 |
+ |
ATP6V0D1 |
NNNAGATTGTAGGCAAGGGT |
NGG |
2 |
0.5 |
Tier II |
| 6 |
NC_000005.10 |
5 |
100861348 |
+ |
ST8SIA4 |
NNNACATTACTGGCAAGGGT |
NGG |
2 |
0.419 |
Tier II |
| 7 |
NC_000017.11 |
17 |
15467237 |
+ |
CDRT4 |
NNNAGCTTGTTGGCAAGGGT |
NGG |
2 |
0.303 |
Tier II |
| 8 |
NC_000017.11 |
17 |
15467237 |
+ |
TVP23C-CDRT4 |
NNNAGCTTGTTGGCAAGGGT |
NGG |
2 |
0.303 |
Tier II |
| 9 |
NC_000007.14 |
7 |
70655873 |
- |
AUTS2 |
NNNAGATTCTTGGCAAGGCT |
NGG |
2 |
0.2562 |
Tier II |
| 10 |
NC_000005.10 |
5 |
176979192 |
+ |
UIMC1 |
NNNAGATTATTGGAAAGTGT |
NGG |
2 |
0.2333 |
Tier II |
| 11 |
NC_000023.11 |
X |
92199708 |
+ |
PCDH11X |
NNNAGAATATTGGCAGGGGT |
NGG |
2 |
0.1683 |
Tier II |
| 12 |
NC_000006.12 |
6 |
154259765 |
- |
IPCEF1 |
NNNAGATTATTGGTAGGGGT |
NGG |
2 |
0.141 |
Tier II |
| 13 |
NC_000005.10 |
5 |
180331049 |
- |
GFPT2 |
NNNAGATTAGTGGCAAGGGT |
NAG |
2 |
0.1296 |
Tier II |
| 14 |
NC_000015.10 |
15 |
63632246 |
- |
HERC1 |
NNNAGATTCTTGGCAAGGGT |
NGT |
2 |
0.0092 |
Tier II |
| 15 |
NC_000003.12 |
3 |
65704886 |
- |
MAGI1 |
NNNGGATTATTGGGAAGGGT |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000005.10 |
5 |
126203448 |
- |
LOC124901056 |
NNNAGATCATTAGCAAGGGT |
NGG |
2 |
0.9333 |
Tier III |
| 17 |
NC_000003.12 |
3 |
20918766 |
- |
LOC107986068 |
NNNAGATTATTAGCAAGGTT |
NGG |
2 |
0.6222 |
Tier III |
| 18 |
NC_000004.12 |
4 |
79090637 |
+ |
LINC01088 |
NNNAGATGGTTGGCAAGGGT |
NGG |
2 |
0.4889 |
Tier III |
| 19 |
NC_000005.10 |
5 |
176979192 |
+ |
LOC124901145 |
NNNAGATTATTGGAAAGTGT |
NGG |
2 |
0.2333 |
Tier III |
| 20 |
NC_000004.12 |
4 |
60795285 |
+ |
LINC02496 |
NNNAGATTGTTGGCAAGGGC |
NGG |
2 |
0.0606 |
Tier III |
| 21 |
NC_000006.12 |
6 |
30018203 |
- |
POLR1HASP |
NNNAGATTACTGGCAAGGGT |
NTG |
2 |
0.0208 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
101198849 |
+ |
Acvr1b |
NNNAGATTATCGGCAAGGGC |
NGG |
2 |
0.0606 |
Tier I |
| 2 |
NC_000067.6 |
1 |
135849311 |
- |
Tnnt2 |
NNNAGATCAATGGCAAGGGT |
NGG |
2 |
0.8571 |
Tier II |
| 3 |
NC_000072.6 |
6 |
77794576 |
- |
Ctnna2 |
NNNAAATTATTGGCAAGAGT |
NGG |
2 |
0.6 |
Tier II |
| 4 |
NC_000078.6 |
12 |
17425302 |
+ |
Nol10 |
NNNAGATTTTTGGCAAAGGT |
NGG |
2 |
0.56 |
Tier II |
| 5 |
NC_000081.6 |
15 |
30808114 |
+ |
Ctnnd2 |
NNNTAATTATTGGCAAGGGT |
NGG |
2 |
0.5515 |
Tier II |
| 6 |
NC_000082.6 |
16 |
12034536 |
+ |
Shisa9 |
NNNAGACTGTTGGCAAGGGT |
NGG |
2 |
0.4583 |
Tier II |
| 7 |
NC_000081.6 |
15 |
55769245 |
+ |
Sntb1 |
NNNAGATTATGGGCAAGGTT |
NGG |
2 |
0.2667 |
Tier II |
| 8 |
NC_000074.6 |
8 |
17119508 |
+ |
Csmd1 |
NNNAGATTGTTGGCAATGGT |
NGG |
2 |
0.1667 |
Tier II |
| 9 |
NC_000082.6 |
16 |
9903796 |
+ |
Grin2a |
NNNAGATTACTGGCAAGGGT |
NAG |
2 |
0.1383 |
Tier II |
| 10 |
NC_000073.6 |
7 |
118937559 |
+ |
Iqck |
NNNAGATTAGTGGCCAGGGT |
NGG |
2 |
0.1364 |
Tier II |
| 11 |
NC_000078.6 |
12 |
53250870 |
+ |
Npas3 |
NNNAGATTATTGGCAGGTGT |
NGG |
2 |
0.1282 |
Tier II |
| 12 |
NC_000067.6 |
1 |
130183354 |
- |
Thsd7b |
NNNAGATAATTGGCAAGGGC |
NGG |
2 |
0.0727 |
Tier II |
| 13 |
NC_000068.7 |
2 |
67863457 |
+ |
B3galt1 |
NNNAGATTATTGGCAAGGGT |
NCC |
2 |
0.0 |
Tier II |
| 14 |
NC_000068.7 |
2 |
155620595 |
- |
Myh7b |
NNNAGATTATTGGCAAGGGT |
NAA |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
