Construct: sgRNA BRDN0001146179

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAGAGATTATTGGCAAGGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACVR1B (91)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76681

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 51981034 + ACVR1B NNNAGATTATTGGCAAGGGT NGG 0 1.0 Tier I
2 NC_000010.11 10 73775576 + POFUT4 NNNAGATTATTGGCAAGGTC NGG 2 0.0606 Tier I
3 NC_000008.11 8 117987379 + EXT1 NNNAGATCAATGGCAAGGGT NGG 2 0.8571 Tier II
4 NC_000008.11 8 98539122 - STK3 NNNAAATAATTGGCAAGGGT NGG 2 0.6933 Tier II
5 NC_000016.10 16 67440564 + ATP6V0D1 NNNAGATTGTAGGCAAGGGT NGG 2 0.5 Tier II
6 NC_000005.10 5 100861348 + ST8SIA4 NNNACATTACTGGCAAGGGT NGG 2 0.419 Tier II
7 NC_000017.11 17 15467237 + CDRT4 NNNAGCTTGTTGGCAAGGGT NGG 2 0.303 Tier II
8 NC_000017.11 17 15467237 + TVP23C-CDRT4 NNNAGCTTGTTGGCAAGGGT NGG 2 0.303 Tier II
9 NC_000007.14 7 70655873 - AUTS2 NNNAGATTCTTGGCAAGGCT NGG 2 0.2562 Tier II
10 NC_000005.10 5 176979192 + UIMC1 NNNAGATTATTGGAAAGTGT NGG 2 0.2333 Tier II
11 NC_000023.11 X 92199708 + PCDH11X NNNAGAATATTGGCAGGGGT NGG 2 0.1683 Tier II
12 NC_000006.12 6 154259765 - IPCEF1 NNNAGATTATTGGTAGGGGT NGG 2 0.141 Tier II
13 NC_000005.10 5 180331049 - GFPT2 NNNAGATTAGTGGCAAGGGT NAG 2 0.1296 Tier II
14 NC_000015.10 15 63632246 - HERC1 NNNAGATTCTTGGCAAGGGT NGT 2 0.0092 Tier II
15 NC_000003.12 3 65704886 - MAGI1 NNNGGATTATTGGGAAGGGT NGG 2 0.0 Tier II
16 NC_000005.10 5 126203448 - LOC124901056 NNNAGATCATTAGCAAGGGT NGG 2 0.9333 Tier III
17 NC_000003.12 3 20918766 - LOC107986068 NNNAGATTATTAGCAAGGTT NGG 2 0.6222 Tier III
18 NC_000004.12 4 79090637 + LINC01088 NNNAGATGGTTGGCAAGGGT NGG 2 0.4889 Tier III
19 NC_000005.10 5 176979192 + LOC124901145 NNNAGATTATTGGAAAGTGT NGG 2 0.2333 Tier III
20 NC_000004.12 4 60795285 + LINC02496 NNNAGATTGTTGGCAAGGGC NGG 2 0.0606 Tier III
21 NC_000006.12 6 30018203 - POLR1HASP NNNAGATTACTGGCAAGGGT NTG 2 0.0208 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 101198849 + Acvr1b NNNAGATTATCGGCAAGGGC NGG 2 0.0606 Tier I
2 NC_000067.6 1 135849311 - Tnnt2 NNNAGATCAATGGCAAGGGT NGG 2 0.8571 Tier II
3 NC_000072.6 6 77794576 - Ctnna2 NNNAAATTATTGGCAAGAGT NGG 2 0.6 Tier II
4 NC_000078.6 12 17425302 + Nol10 NNNAGATTTTTGGCAAAGGT NGG 2 0.56 Tier II
5 NC_000081.6 15 30808114 + Ctnnd2 NNNTAATTATTGGCAAGGGT NGG 2 0.5515 Tier II
6 NC_000082.6 16 12034536 + Shisa9 NNNAGACTGTTGGCAAGGGT NGG 2 0.4583 Tier II
7 NC_000081.6 15 55769245 + Sntb1 NNNAGATTATGGGCAAGGTT NGG 2 0.2667 Tier II
8 NC_000074.6 8 17119508 + Csmd1 NNNAGATTGTTGGCAATGGT NGG 2 0.1667 Tier II
9 NC_000082.6 16 9903796 + Grin2a NNNAGATTACTGGCAAGGGT NAG 2 0.1383 Tier II
10 NC_000073.6 7 118937559 + Iqck NNNAGATTAGTGGCCAGGGT NGG 2 0.1364 Tier II
11 NC_000078.6 12 53250870 + Npas3 NNNAGATTATTGGCAGGTGT NGG 2 0.1282 Tier II
12 NC_000067.6 1 130183354 - Thsd7b NNNAGATAATTGGCAAGGGC NGG 2 0.0727 Tier II
13 NC_000068.7 2 67863457 + B3galt1 NNNAGATTATTGGCAAGGGT NCC 2 0.0 Tier II
14 NC_000068.7 2 155620595 - Myh7b NNNAGATTATTGGCAAGGGT NAA 2 0.0 Tier II
Download CSV

Other clones with same target sequence:

(none)

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