Construct: sgRNA BRDN0001146180

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACAATGTCAGAGGACCACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEK4 (6787)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77710

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 52752019 + NEK4 NNNATGTCAGAGGACCACAT NGG 0 1.0 Tier I
2 NC_000016.10 16 30750611 + PHKG2 NNNATGTCAGGGGACCACTT NGG 2 0.35 Tier II
3 NC_000021.9 21 10568668 - TPTE NNNTAGTCAGAGGACCACAT NGG 2 0.3182 Tier II
4 NC_000012.12 12 43787499 + IRAK4 NNNATGTGAGAGGGCCACAT NGG 2 0.3 Tier II
5 NC_000018.10 18 74734048 + ZNF407 NNNCTGTCAGAGGCCCACAT NGG 2 0.0756 Tier II
6 NC_000009.12 9 112351796 - PTBP3 NNNATGTCAAAGGAGCACAT NGG 2 0.0467 Tier II
7 NC_000001.11 1 217050138 - ESRRG NNNAGGTCAGAGGATCACAT NGG 2 0.0427 Tier II
8 NC_000009.12 9 38580316 + ANKRD18A NNNAGGTCAGAGGATCACAT NGG 2 0.0427 Tier II
9 NC_000012.12 12 61866733 - TAFA2 NNNATGTCAGAGGACCACTT NGA 2 0.0374 Tier II
10 NC_000008.11 8 134502760 + ZFAT NNNATGTCAGAGGAGCACTT NGG 2 0.0269 Tier II
11 NC_000010.11 10 124727981 + FAM53B NNNATGTCTGAGGACCACAT NTG 2 0.0234 Tier II
12 NC_000015.10 15 43878846 + FRMD5 NNNATGTCAGAGGACCACCT NGA 2 0.0144 Tier II
13 NC_000013.11 13 52281757 + TPTE2P2 NNNTAGTCAGAGGACCACAT NGG 2 0.3182 Tier III
14 NC_000013.11 13 52530858 - TPTE2P3 NNNTAGTCAGAGGACCACAT NGG 2 0.3182 Tier III
15 NC_000013.11 13 52530858 - LINC00345 NNNTAGTCAGAGGACCACAT NGG 2 0.3182 Tier III
16 NC_000003.12 3 153669165 + LINC02006 NNNATTTCAGTGGACCACAT NGG 2 0.2051 Tier III
17 NC_000003.12 3 90234718 + PROS2P NNNATGGCAGAGGACTACAT NGG 2 0.181 Tier III
18 NC_000004.12 4 14780637 + LINC00504 NNNATGTAAGAGGACCACAG NGG 2 0.1147 Tier III
19 NC_000009.12 9 97164995 + ANKRD18CP NNNAGGTCAGAGGATCACAT NGG 2 0.0427 Tier III
20 NC_000004.12 4 154154578 - LOC101927947 NNNATGTCAGAGGAGCACAT NAG 2 0.013 Tier III
21 NC_000008.11 8 116198674 - LINC00536 NNNATGTCAGAGGAGCACAT NAG 2 0.013 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 119145370 + Gpr139 NNNATGTCAGAGAACCACAT NGG 1 0.9231 Tier II
2 NC_000082.6 16 55887429 - Nxpe3 NNNATGTCAGAGGACAATAT NGG 2 0.6429 Tier II
3 NC_000068.7 2 67590051 - B3galt1 NNNAGGTCAGAGGACAACAT NGG 2 0.64 Tier II
4 NC_000071.6 5 8650737 + Abcb1a NNNAGGTCAGAGGACAACAT NGG 2 0.64 Tier II
5 NC_000075.6 9 9884519 - Cntn5 NNNAGGTCAGAGGACAACAT NGG 2 0.64 Tier II
6 NC_000080.6 14 13443689 + Synpr NNNAGGTCAGAGGACAACAT NGG 2 0.64 Tier II
7 NC_000085.6 19 27412416 - Pum3 NNNAGGTCAGAGGACAACAT NGG 2 0.64 Tier II
8 NC_000080.6 14 27849508 - Erc2 NNNTTATCAGAGGACCACAT NGG 2 0.6364 Tier II
9 NC_000071.6 5 100832223 - Abraxas1 NNNATTACAGAGGACCACAT NGG 2 0.5833 Tier II
10 NC_000074.6 8 57872281 + Galntl6 NNNAGGACAGAGGACCACAT NGG 2 0.56 Tier II
11 NC_000075.6 9 108429309 + Iho1 NNNATGTCTAAGGACCACAT NGG 2 0.56 Tier II
12 NC_000069.6 3 115989890 - Slc30a7 NNNATGTCAGAGGACAACTT NGG 2 0.5385 Tier II
13 NC_000071.6 5 107130315 + Tgfbr3 NNNATGTCAGAGGACAACTT NGG 2 0.5385 Tier II
14 NC_000077.6 11 93113493 + Car10 NNNATGTCAGAGGACAACTT NGG 2 0.5385 Tier II
15 NC_000084.6 18 34427852 - Pkd2l2 NNNATGTCAGAGGACAACTT NGG 2 0.5385 Tier II
16 NC_000068.7 2 181197753 + Ptk6 NNNAAGTCAGAGGACAACAT NGG 2 0.5 Tier II
17 NC_000068.7 2 18122883 - Mllt10 NNNATGTCAGAGAACCACTT NGG 2 0.497 Tier II
18 NC_000079.6 13 15637283 - Gli3 NNNAAGTCAGAGAACCACAT NGG 2 0.4615 Tier II
19 NC_000072.6 6 32542718 + Plxna4 NNNATGTCAGAGCACAACAT NGG 2 0.4211 Tier II
20 NC_000067.6 1 84682557 - Dner NNNAGGTCAGAGGACCACTT NGG 2 0.3446 Tier II
21 NC_000069.6 3 25098494 - Naaladl2 NNNAGGTCAGAGGACCACTT NGG 2 0.3446 Tier II
22 NC_000072.6 6 72141647 + St3gal5 NNNAGGTCAGAGGACCACTT NGG 2 0.3446 Tier II
23 NC_000082.6 16 96173103 + Lca5l NNNAGGTCAGAGGACCACTT NGG 2 0.3446 Tier II
24 NC_000083.6 17 66071846 + Ankrd12 NNNAGGTCAGAGGACCACTT NGG 2 0.3446 Tier II
25 NC_000079.6 13 74107441 - Slc9a3 NNNAAGTCAGAGGACCACAA NGG 2 0.2813 Tier II
26 NC_000067.6 1 74113631 - Tns1 NNNATGTAACAGGACCACAT NGG 2 0.26 Tier II
27 NC_000076.6 10 119589023 + Grip1 NNNTTGTCACAGGACCACAT NGG 2 0.2545 Tier II
28 NC_000083.6 17 24152924 - Pdpk1 NNNATGTCTGAGCACCACAT NGG 2 0.2526 Tier II
29 NC_000074.6 8 78652676 + Slc10a7 NNNAGGTCAGAGGACCACGT NGG 2 0.24 Tier II
30 NC_000072.6 6 138148450 - Mgst1 NNNTTGTCAGAGGACCACGT NGG 2 0.2386 Tier II
31 NC_000067.6 1 167606400 - Rxrg NNNATGTCAGAGGACAACCT NGG 2 0.2069 Tier II
32 NC_000069.6 3 123219938 - Synpo2 NNNATGTCAGAGGACAACCT NGG 2 0.2069 Tier II
33 NC_000070.6 4 62295290 + Slc31a2 NNNATGTCAGAGGACAACCT NGG 2 0.2069 Tier II
34 NC_000071.6 5 136514821 + Cux1 NNNATGTCAGAGGACAACCT NGG 2 0.2069 Tier II
35 NC_000073.6 7 76231200 + Agbl1 NNNATGTCAGAGGACAACCT NGG 2 0.2069 Tier II
36 NC_000075.6 9 73970596 - Unc13c NNNATGTCAGAGGACAACCT NGG 2 0.2069 Tier II
37 NC_000079.6 13 9974867 + Chrm3 NNNATGTCAGAGGACAACCT NGG 2 0.2069 Tier II
38 NC_000081.6 15 78401874 + Tst NNNATGTCAGAGGACCACCT NGG 1 0.2069 Tier II
39 NC_000076.6 10 120068431 - Grip1 NNNAGGTCAGAGGACTACAT NGG 2 0.1969 Tier II
40 NC_000074.6 8 78226933 - Ttc29 NNNGTGTCAGAGGACTACAT NGG 2 0.1923 Tier II
41 NC_000067.6 1 60212445 - Nbeal1 NNNATGTCAGAGGACTACTT NGG 2 0.1657 Tier II
42 NC_000067.6 1 70297565 + Spag16 NNNATGTCGGAGGAACACAT NGG 2 0.1481 Tier II
43 NC_000072.6 6 22330991 - Fam3c NNNAGGTCAGAGGACCACCT NGG 2 0.1324 Tier II
44 NC_000083.6 17 30047881 + Zfand3 NNNATGTCAGTGCACCACAT NGG 2 0.1296 Tier II
45 NC_000083.6 17 72376694 - Alk NNNGTGTCAGAGGACCACCT NGG 2 0.1293 Tier II
46 NC_000076.6 10 34327619 + Nt5dc1 NNNAGGTCAGAGGACCACAG NGG 2 0.1129 Tier II
47 NC_000074.6 8 111650521 + Zfp1 NNNAAGTCAGAGGACCAGAT NGG 2 0.0667 Tier II
48 NC_000068.7 2 53043237 - Fmnl2 NNNATGTCAGAGGAGAACAT NGG 2 0.05 Tier II
49 NC_000068.7 2 66186963 + Ttc21b NNNATGTCAGAGGAACACCT NGG 2 0.046 Tier II
50 NC_000079.6 13 50243909 + Gm906 NNNATGACAGAGGAGCACAT NGG 2 0.0438 Tier II
51 NC_000077.6 11 35897474 + Wwc1 NNNATGGCAGAGGACCACAT NGA 2 0.0408 Tier II
52 NC_000074.6 8 84578123 - Cacna1a NNNATGTCAGAGGACAACAT NTG 2 0.039 Tier II
53 NC_000068.7 2 29373039 - Med27 NNNATGTCAGAGGCCGACAT NGG 2 0.033 Tier II
54 NC_000072.6 6 91048857 - Nup210 NNNAAGTCAGAGGAGCACAT NGG 2 0.025 Tier II
55 NC_000074.6 8 13482207 + Gas6 NNNCTGTCAGAGGATCACAT NGG 2 0.0235 Tier II
56 NC_000074.6 8 13482207 + Tmem255b NNNCTGTCAGAGGATCACAT NGG 2 0.0235 Tier II
57 NC_000077.6 11 73256403 + Trpv1 NNNATGACAGAGGACCACAT NGC 2 0.0194 Tier II
58 NC_000082.6 16 21490096 + Vps8 NNNATGTCAGAAGACCACAT NGT 2 0.0151 Tier II
59 NC_000072.6 6 138148466 - Mgst1 NNNTTGTCAGAGGACCACAT NGT 2 0.0103 Tier II
60 NC_000072.6 6 138148482 - Mgst1 NNNTTGTCAGAGGACCACAT NGT 2 0.0103 Tier II
61 NC_000068.7 2 127333059 + Gm46762 NNNACATCAGAGGACCACAT NGG 2 1.0 Tier III
62 NC_000076.6 10 66315869 - Gm40677 NNNATGTCAGAAGACAACAT NGG 2 0.9333 Tier III
63 NC_000081.6 15 102659678 - Gm4544 NNNATGTCAGAGGACAACTT NGG 2 0.5385 Tier III
64 NC_000083.6 17 51936285 - Gm20098 NNNGTGTCCGAGGACCACAT NGG 2 0.3571 Tier III
65 NC_000078.6 12 26140270 - Gm29687 NNNATGTCAGAGGACAACCT NGG 2 0.2069 Tier III
66 NC_000074.6 8 128230163 + A930035F09Rik NNNAGGTCAGAGGACCACCT NGG 2 0.1324 Tier III
67 NC_000075.6 9 120220778 + Gm38642 NNNAGGTCAGAGGACCGCAT NGG 2 0.1129 Tier III
68 NC_000067.6 1 35872472 - 1110002O04Rik NNNGTGTCAGAGGAGCACAT NGG 2 0.0313 Tier III
69 NC_000079.6 13 57406252 + Gm33294 NNNAGGTCAGAGGACCACAT NTG 2 0.0249 Tier III
70 NC_000072.6 6 135288157 + Gm36640 NNNATGTCAGAGGACCACTT NTG 2 0.021 Tier III
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Other clones with same target sequence:

(none)