Construct: sgRNA BRDN0001146180
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACAATGTCAGAGGACCACAT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- NEK4 (6787)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000003.12 | 3 | 52752019 | + | NEK4 | NNNATGTCAGAGGACCACAT | NGG | 0 | 1.0 | Tier I |
2 | NC_000016.10 | 16 | 30750611 | + | PHKG2 | NNNATGTCAGGGGACCACTT | NGG | 2 | 0.35 | Tier II |
3 | NC_000021.9 | 21 | 10568668 | - | TPTE | NNNTAGTCAGAGGACCACAT | NGG | 2 | 0.3182 | Tier II |
4 | NC_000012.12 | 12 | 43787499 | + | IRAK4 | NNNATGTGAGAGGGCCACAT | NGG | 2 | 0.3 | Tier II |
5 | NC_000018.10 | 18 | 74734048 | + | ZNF407 | NNNCTGTCAGAGGCCCACAT | NGG | 2 | 0.0756 | Tier II |
6 | NC_000009.12 | 9 | 112351796 | - | PTBP3 | NNNATGTCAAAGGAGCACAT | NGG | 2 | 0.0467 | Tier II |
7 | NC_000001.11 | 1 | 217050138 | - | ESRRG | NNNAGGTCAGAGGATCACAT | NGG | 2 | 0.0427 | Tier II |
8 | NC_000009.12 | 9 | 38580316 | + | ANKRD18A | NNNAGGTCAGAGGATCACAT | NGG | 2 | 0.0427 | Tier II |
9 | NC_000012.12 | 12 | 61866733 | - | TAFA2 | NNNATGTCAGAGGACCACTT | NGA | 2 | 0.0374 | Tier II |
10 | NC_000008.11 | 8 | 134502760 | + | ZFAT | NNNATGTCAGAGGAGCACTT | NGG | 2 | 0.0269 | Tier II |
11 | NC_000010.11 | 10 | 124727981 | + | FAM53B | NNNATGTCTGAGGACCACAT | NTG | 2 | 0.0234 | Tier II |
12 | NC_000015.10 | 15 | 43878846 | + | FRMD5 | NNNATGTCAGAGGACCACCT | NGA | 2 | 0.0144 | Tier II |
13 | NC_000013.11 | 13 | 52281757 | + | TPTE2P2 | NNNTAGTCAGAGGACCACAT | NGG | 2 | 0.3182 | Tier III |
14 | NC_000013.11 | 13 | 52530858 | - | TPTE2P3 | NNNTAGTCAGAGGACCACAT | NGG | 2 | 0.3182 | Tier III |
15 | NC_000013.11 | 13 | 52530858 | - | LINC00345 | NNNTAGTCAGAGGACCACAT | NGG | 2 | 0.3182 | Tier III |
16 | NC_000003.12 | 3 | 153669165 | + | LINC02006 | NNNATTTCAGTGGACCACAT | NGG | 2 | 0.2051 | Tier III |
17 | NC_000003.12 | 3 | 90234718 | + | PROS2P | NNNATGGCAGAGGACTACAT | NGG | 2 | 0.181 | Tier III |
18 | NC_000004.12 | 4 | 14780637 | + | LINC00504 | NNNATGTAAGAGGACCACAG | NGG | 2 | 0.1147 | Tier III |
19 | NC_000009.12 | 9 | 97164995 | + | ANKRD18CP | NNNAGGTCAGAGGATCACAT | NGG | 2 | 0.0427 | Tier III |
20 | NC_000004.12 | 4 | 154154578 | - | LOC101927947 | NNNATGTCAGAGGAGCACAT | NAG | 2 | 0.013 | Tier III |
21 | NC_000008.11 | 8 | 116198674 | - | LINC00536 | NNNATGTCAGAGGAGCACAT | NAG | 2 | 0.013 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000073.6 | 7 | 119145370 | + | Gpr139 | NNNATGTCAGAGAACCACAT | NGG | 1 | 0.9231 | Tier II |
2 | NC_000082.6 | 16 | 55887429 | - | Nxpe3 | NNNATGTCAGAGGACAATAT | NGG | 2 | 0.6429 | Tier II |
3 | NC_000068.7 | 2 | 67590051 | - | B3galt1 | NNNAGGTCAGAGGACAACAT | NGG | 2 | 0.64 | Tier II |
4 | NC_000071.6 | 5 | 8650737 | + | Abcb1a | NNNAGGTCAGAGGACAACAT | NGG | 2 | 0.64 | Tier II |
5 | NC_000075.6 | 9 | 9884519 | - | Cntn5 | NNNAGGTCAGAGGACAACAT | NGG | 2 | 0.64 | Tier II |
6 | NC_000080.6 | 14 | 13443689 | + | Synpr | NNNAGGTCAGAGGACAACAT | NGG | 2 | 0.64 | Tier II |
7 | NC_000085.6 | 19 | 27412416 | - | Pum3 | NNNAGGTCAGAGGACAACAT | NGG | 2 | 0.64 | Tier II |
8 | NC_000080.6 | 14 | 27849508 | - | Erc2 | NNNTTATCAGAGGACCACAT | NGG | 2 | 0.6364 | Tier II |
9 | NC_000071.6 | 5 | 100832223 | - | Abraxas1 | NNNATTACAGAGGACCACAT | NGG | 2 | 0.5833 | Tier II |
10 | NC_000074.6 | 8 | 57872281 | + | Galntl6 | NNNAGGACAGAGGACCACAT | NGG | 2 | 0.56 | Tier II |
11 | NC_000075.6 | 9 | 108429309 | + | Iho1 | NNNATGTCTAAGGACCACAT | NGG | 2 | 0.56 | Tier II |
12 | NC_000069.6 | 3 | 115989890 | - | Slc30a7 | NNNATGTCAGAGGACAACTT | NGG | 2 | 0.5385 | Tier II |
13 | NC_000071.6 | 5 | 107130315 | + | Tgfbr3 | NNNATGTCAGAGGACAACTT | NGG | 2 | 0.5385 | Tier II |
14 | NC_000077.6 | 11 | 93113493 | + | Car10 | NNNATGTCAGAGGACAACTT | NGG | 2 | 0.5385 | Tier II |
15 | NC_000084.6 | 18 | 34427852 | - | Pkd2l2 | NNNATGTCAGAGGACAACTT | NGG | 2 | 0.5385 | Tier II |
16 | NC_000068.7 | 2 | 181197753 | + | Ptk6 | NNNAAGTCAGAGGACAACAT | NGG | 2 | 0.5 | Tier II |
17 | NC_000068.7 | 2 | 18122883 | - | Mllt10 | NNNATGTCAGAGAACCACTT | NGG | 2 | 0.497 | Tier II |
18 | NC_000079.6 | 13 | 15637283 | - | Gli3 | NNNAAGTCAGAGAACCACAT | NGG | 2 | 0.4615 | Tier II |
19 | NC_000072.6 | 6 | 32542718 | + | Plxna4 | NNNATGTCAGAGCACAACAT | NGG | 2 | 0.4211 | Tier II |
20 | NC_000067.6 | 1 | 84682557 | - | Dner | NNNAGGTCAGAGGACCACTT | NGG | 2 | 0.3446 | Tier II |
21 | NC_000069.6 | 3 | 25098494 | - | Naaladl2 | NNNAGGTCAGAGGACCACTT | NGG | 2 | 0.3446 | Tier II |
22 | NC_000072.6 | 6 | 72141647 | + | St3gal5 | NNNAGGTCAGAGGACCACTT | NGG | 2 | 0.3446 | Tier II |
23 | NC_000082.6 | 16 | 96173103 | + | Lca5l | NNNAGGTCAGAGGACCACTT | NGG | 2 | 0.3446 | Tier II |
24 | NC_000083.6 | 17 | 66071846 | + | Ankrd12 | NNNAGGTCAGAGGACCACTT | NGG | 2 | 0.3446 | Tier II |
25 | NC_000079.6 | 13 | 74107441 | - | Slc9a3 | NNNAAGTCAGAGGACCACAA | NGG | 2 | 0.2813 | Tier II |
26 | NC_000067.6 | 1 | 74113631 | - | Tns1 | NNNATGTAACAGGACCACAT | NGG | 2 | 0.26 | Tier II |
27 | NC_000076.6 | 10 | 119589023 | + | Grip1 | NNNTTGTCACAGGACCACAT | NGG | 2 | 0.2545 | Tier II |
28 | NC_000083.6 | 17 | 24152924 | - | Pdpk1 | NNNATGTCTGAGCACCACAT | NGG | 2 | 0.2526 | Tier II |
29 | NC_000074.6 | 8 | 78652676 | + | Slc10a7 | NNNAGGTCAGAGGACCACGT | NGG | 2 | 0.24 | Tier II |
30 | NC_000072.6 | 6 | 138148450 | - | Mgst1 | NNNTTGTCAGAGGACCACGT | NGG | 2 | 0.2386 | Tier II |
31 | NC_000067.6 | 1 | 167606400 | - | Rxrg | NNNATGTCAGAGGACAACCT | NGG | 2 | 0.2069 | Tier II |
32 | NC_000069.6 | 3 | 123219938 | - | Synpo2 | NNNATGTCAGAGGACAACCT | NGG | 2 | 0.2069 | Tier II |
33 | NC_000070.6 | 4 | 62295290 | + | Slc31a2 | NNNATGTCAGAGGACAACCT | NGG | 2 | 0.2069 | Tier II |
34 | NC_000071.6 | 5 | 136514821 | + | Cux1 | NNNATGTCAGAGGACAACCT | NGG | 2 | 0.2069 | Tier II |
35 | NC_000073.6 | 7 | 76231200 | + | Agbl1 | NNNATGTCAGAGGACAACCT | NGG | 2 | 0.2069 | Tier II |
36 | NC_000075.6 | 9 | 73970596 | - | Unc13c | NNNATGTCAGAGGACAACCT | NGG | 2 | 0.2069 | Tier II |
37 | NC_000079.6 | 13 | 9974867 | + | Chrm3 | NNNATGTCAGAGGACAACCT | NGG | 2 | 0.2069 | Tier II |
38 | NC_000081.6 | 15 | 78401874 | + | Tst | NNNATGTCAGAGGACCACCT | NGG | 1 | 0.2069 | Tier II |
39 | NC_000076.6 | 10 | 120068431 | - | Grip1 | NNNAGGTCAGAGGACTACAT | NGG | 2 | 0.1969 | Tier II |
40 | NC_000074.6 | 8 | 78226933 | - | Ttc29 | NNNGTGTCAGAGGACTACAT | NGG | 2 | 0.1923 | Tier II |
41 | NC_000067.6 | 1 | 60212445 | - | Nbeal1 | NNNATGTCAGAGGACTACTT | NGG | 2 | 0.1657 | Tier II |
42 | NC_000067.6 | 1 | 70297565 | + | Spag16 | NNNATGTCGGAGGAACACAT | NGG | 2 | 0.1481 | Tier II |
43 | NC_000072.6 | 6 | 22330991 | - | Fam3c | NNNAGGTCAGAGGACCACCT | NGG | 2 | 0.1324 | Tier II |
44 | NC_000083.6 | 17 | 30047881 | + | Zfand3 | NNNATGTCAGTGCACCACAT | NGG | 2 | 0.1296 | Tier II |
45 | NC_000083.6 | 17 | 72376694 | - | Alk | NNNGTGTCAGAGGACCACCT | NGG | 2 | 0.1293 | Tier II |
46 | NC_000076.6 | 10 | 34327619 | + | Nt5dc1 | NNNAGGTCAGAGGACCACAG | NGG | 2 | 0.1129 | Tier II |
47 | NC_000074.6 | 8 | 111650521 | + | Zfp1 | NNNAAGTCAGAGGACCAGAT | NGG | 2 | 0.0667 | Tier II |
48 | NC_000068.7 | 2 | 53043237 | - | Fmnl2 | NNNATGTCAGAGGAGAACAT | NGG | 2 | 0.05 | Tier II |
49 | NC_000068.7 | 2 | 66186963 | + | Ttc21b | NNNATGTCAGAGGAACACCT | NGG | 2 | 0.046 | Tier II |
50 | NC_000079.6 | 13 | 50243909 | + | Gm906 | NNNATGACAGAGGAGCACAT | NGG | 2 | 0.0438 | Tier II |
51 | NC_000077.6 | 11 | 35897474 | + | Wwc1 | NNNATGGCAGAGGACCACAT | NGA | 2 | 0.0408 | Tier II |
52 | NC_000074.6 | 8 | 84578123 | - | Cacna1a | NNNATGTCAGAGGACAACAT | NTG | 2 | 0.039 | Tier II |
53 | NC_000068.7 | 2 | 29373039 | - | Med27 | NNNATGTCAGAGGCCGACAT | NGG | 2 | 0.033 | Tier II |
54 | NC_000072.6 | 6 | 91048857 | - | Nup210 | NNNAAGTCAGAGGAGCACAT | NGG | 2 | 0.025 | Tier II |
55 | NC_000074.6 | 8 | 13482207 | + | Gas6 | NNNCTGTCAGAGGATCACAT | NGG | 2 | 0.0235 | Tier II |
56 | NC_000074.6 | 8 | 13482207 | + | Tmem255b | NNNCTGTCAGAGGATCACAT | NGG | 2 | 0.0235 | Tier II |
57 | NC_000077.6 | 11 | 73256403 | + | Trpv1 | NNNATGACAGAGGACCACAT | NGC | 2 | 0.0194 | Tier II |
58 | NC_000082.6 | 16 | 21490096 | + | Vps8 | NNNATGTCAGAAGACCACAT | NGT | 2 | 0.0151 | Tier II |
59 | NC_000072.6 | 6 | 138148466 | - | Mgst1 | NNNTTGTCAGAGGACCACAT | NGT | 2 | 0.0103 | Tier II |
60 | NC_000072.6 | 6 | 138148482 | - | Mgst1 | NNNTTGTCAGAGGACCACAT | NGT | 2 | 0.0103 | Tier II |
61 | NC_000068.7 | 2 | 127333059 | + | Gm46762 | NNNACATCAGAGGACCACAT | NGG | 2 | 1.0 | Tier III |
62 | NC_000076.6 | 10 | 66315869 | - | Gm40677 | NNNATGTCAGAAGACAACAT | NGG | 2 | 0.9333 | Tier III |
63 | NC_000081.6 | 15 | 102659678 | - | Gm4544 | NNNATGTCAGAGGACAACTT | NGG | 2 | 0.5385 | Tier III |
64 | NC_000083.6 | 17 | 51936285 | - | Gm20098 | NNNGTGTCCGAGGACCACAT | NGG | 2 | 0.3571 | Tier III |
65 | NC_000078.6 | 12 | 26140270 | - | Gm29687 | NNNATGTCAGAGGACAACCT | NGG | 2 | 0.2069 | Tier III |
66 | NC_000074.6 | 8 | 128230163 | + | A930035F09Rik | NNNAGGTCAGAGGACCACCT | NGG | 2 | 0.1324 | Tier III |
67 | NC_000075.6 | 9 | 120220778 | + | Gm38642 | NNNAGGTCAGAGGACCGCAT | NGG | 2 | 0.1129 | Tier III |
68 | NC_000067.6 | 1 | 35872472 | - | 1110002O04Rik | NNNGTGTCAGAGGAGCACAT | NGG | 2 | 0.0313 | Tier III |
69 | NC_000079.6 | 13 | 57406252 | + | Gm33294 | NNNAGGTCAGAGGACCACAT | NTG | 2 | 0.0249 | Tier III |
70 | NC_000072.6 | 6 | 135288157 | + | Gm36640 | NNNATGTCAGAGGACCACTT | NTG | 2 | 0.021 | Tier III |