Construct: sgRNA BRDN0001146184
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTTCCTTGTAGGCCGAAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDK13 (8621)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76627
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
39951512 |
- |
CDK13 |
NNNTCCTTGTAGGCCGAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
153415414 |
- |
S100A8 |
NNNTCCTTGTAGGCCGCAAG |
NGG |
2 |
0.1261 |
Tier II |
| 3 |
NC_000020.11 |
20 |
20295242 |
+ |
CFAP61 |
NNNTCCTTGTAAGCTGAAGG |
NGG |
2 |
0.0622 |
Tier II |
| 4 |
NC_000023.11 |
X |
86749053 |
- |
DACH2 |
NNNTCCTTGTAGGCTGAAAG |
NGG |
2 |
0.0476 |
Tier II |
| 5 |
NC_000017.11 |
17 |
74962002 |
+ |
HID1 |
NNNTCCTTGTAGGAGGAAGG |
NGG |
2 |
0.0175 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
17803782 |
+ |
Cdk13 |
NNNTCCTTGTAGGCCGAGGG |
NGG |
1 |
0.4 |
Tier I |
| 2 |
NC_000082.6 |
16 |
93919315 |
+ |
Cldn14 |
NNNTCCTTGTAGGCCGCTGG |
NGG |
2 |
0.0882 |
Tier I |
| 3 |
NC_000071.6 |
5 |
111289605 |
- |
Ttc28 |
NNNTCCATGTAGGCCAAAGG |
NGG |
2 |
0.875 |
Tier II |
| 4 |
NC_000080.6 |
14 |
69543815 |
- |
LOC115488278 |
NNNTGCTTGTAGGCAGAAGG |
NGG |
2 |
0.1333 |
Tier II |
| 5 |
NC_000073.6 |
7 |
134465967 |
+ |
D7Ertd443e |
NNNTCCTTGTAGGCCCAAGG |
NAG |
2 |
0.0 |
Tier II |
| 6 |
NC_000082.6 |
16 |
3778137 |
- |
Gm41409 |
NNNTCCTTGTAGTCCTAAGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
