Construct: sgRNA BRDN0001146185

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCAATGCAGAGAACCCGAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRC (6714)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75651

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000020.11 20 37394238 + SRC NNNATGCAGAGAACCCGAGA NGG 0 1.0 Tier I
2 NC_000010.11 10 133240008 - VENTX NNNATGGAGAGACCCCGAGA NGG 2 0.0991 Tier I
3 NC_000017.11 17 45408709 - ARHGAP27 NNNAGGCAGAGAACCAGAGA NGG 2 0.64 Tier II
4 NC_000023.11 X 103083288 + NXF3 NNNCTGCAGAGAACCCAAGA NGG 2 0.3294 Tier II
5 NC_000001.11 1 156072083 - MEX3A NNNATGCAGAGAAACCAAGA NGG 2 0.3267 Tier II
6 NC_000011.10 11 64648998 - NRXN2 NNNACGCAGAGAACCTGAGA NGG 2 0.3077 Tier II
7 NC_000010.11 10 60658612 - ANK3 NNNATGCAGAGCACCAGAGA NGG 2 0.2632 Tier II
8 NC_000012.12 12 3834760 + PARP11 NNNATGCAGAGAACCAGAGA NAG 2 0.2593 Tier II
9 NC_000010.11 10 75063669 - DUSP29 NNNATGCAGAGCACCCAAGA NGG 2 0.2456 Tier II
10 NC_000009.12 9 4637263 - SPATA6L NNNATGCAGAGACCCAGAGA NGG 2 0.2105 Tier II
11 NC_000009.12 9 130449740 + ASS1 NNNATGAAGAGAACCCCAGA NGG 2 0.1765 Tier II
12 NC_000022.11 22 43488381 + MPPED1 NNNAGGCAGAGAACCCGAGC NGG 2 0.1455 Tier II
13 NC_000006.12 6 61864772 - KHDRBS2 NNNATGCAGAGAACCCCAGT NGG 2 0.1412 Tier II
14 NC_000012.12 12 111043379 + CUX2 NNNTTGCAGAGACCCCGAGA NGG 2 0.134 Tier II
15 NC_000017.11 17 11490236 + SHISA6 NNNCTGCAGAGTACCCGAGA NGG 2 0.1176 Tier II
16 NC_000014.9 14 92374111 + SLC24A4 NNNATGCAGAGAACCCCAGA NAG 2 0.061 Tier II
17 NC_000014.9 14 56647089 - TMEM260 NNNATGCAGAGAACCCAAGA NTG 2 0.0364 Tier II
18 NC_000010.11 10 132779565 - INPP5A NNNATCCAGAGAACCCGAGA NGC 2 0.0152 Tier II
19 NC_000006.12 6 2363884 + GMDS-DT NNNATGCAGAGAATCCGGGA NGG 2 0.2933 Tier III
20 NC_000022.11 22 22730577 + IGL NNNAAGCAGAGAACCCGTGA NGG 2 0.25 Tier III
21 NC_000011.10 11 46856199 + LRP4-AS1 NNNATCCAGAGAACCTGAGA NGG 2 0.2098 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 58956819 + Zkscan16 NNNATGCGGTGAACCCGAGA NGG 2 0.6471 Tier I
2 NC_000068.7 2 157463043 + Src NNNACGCCGAGAACCCGAGA NGG 2 0.4286 Tier I
3 NC_000068.7 2 51925674 - Rbm43 NNNATGGAGAGAACCCTAGA NGG 2 0.1176 Tier I
4 NC_000075.6 9 15323479 + Cep295 NNNATGCAGAGAACCAGAGA NGG 1 1.0 Tier II
5 NC_000073.6 7 28426418 - Samd4b NNNTTGCAGAGAACCAGAGA NGG 2 0.6364 Tier II
6 NC_000072.6 6 54701786 + Mturn NNNATGCAGGGAACCCAAGA NGG 2 0.5185 Tier II
7 NC_000068.7 2 3757889 - Fam107b NNNTTGCAGAGGACCCGAGA NGG 2 0.4596 Tier II
8 NC_000078.6 12 75997215 + Syne2 NNNTTGCAGAGAACCCGAGT NGG 2 0.3818 Tier II
9 NC_000067.6 1 87087864 + Alppl2 NNNCTGCAGAGAACCAGAGA NGG 2 0.3529 Tier II
10 NC_000069.6 3 88541325 + Mex3a NNNATGCAGAGAAACCAAGA NGG 2 0.3267 Tier II
11 NC_000074.6 8 104191725 - Bean1 NNNATGCAGAGAACCCAAGA NAG 2 0.242 Tier II
12 NC_000069.6 3 118929079 - Dpyd NNNATCCAGAGAACCTGAGA NGG 2 0.2098 Tier II
13 NC_000073.6 7 113528699 - Far1 NNNTTGCAGAGAACCTGAGA NGG 2 0.1958 Tier II
14 NC_000084.6 18 22732956 + Nol4 NNNGTGCAGAGATCCCGAGA NGG 2 0.1875 Tier II
15 NC_000068.7 2 163534735 - Hnf4a NNNTTGCAGAGAACCCTAGA NGG 2 0.1591 Tier II
16 NC_000083.6 17 44766597 - Runx2 NNNATGCTGAGAACCTGAGA NGG 2 0.1319 Tier II
17 NC_000068.7 2 125363353 + Fbn1 NNNATGCAGACACCCCGAGA NGG 2 0.0902 Tier II
18 NC_000072.6 6 50228022 - Gsdme NNNATGCAGTGAACCCGAGA NGC 2 0.0196 Tier II
19 NC_000069.6 3 65743059 - Lekr1 NNNATGCAGAGAACCCCAGA NTG 2 0.0092 Tier II
20 NC_000067.6 1 139115698 - Dennd1b NNNATGCAGAGAAGCCGAGC NGG 2 0.0 Tier II
21 NC_000082.6 16 93505883 + Gm30695 NNNATGCAGAGAACCAGACA NGG 2 0.4483 Tier III
22 NC_000067.6 1 87087864 + Gm29371 NNNCTGCAGAGAACCAGAGA NGG 2 0.3529 Tier III
23 NC_000074.6 8 124080741 - Gm30005 NNNATGCAGTGAACCTGAGA NGG 2 0.2715 Tier III
24 NC_000076.6 10 91896902 + Gm31592 NNNATGCAGAGAACCCCAGA NGG 1 0.2353 Tier III
25 NC_000068.7 2 163534735 - Hnf4aos NNNTTGCAGAGAACCCTAGA NGG 2 0.1591 Tier III
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Other clones with same target sequence:

(none)