Construct: sgRNA BRDN0001146185
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCAATGCAGAGAACCCGAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SRC (6714)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75651
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000020.11 |
20 |
37394238 |
+ |
SRC |
NNNATGCAGAGAACCCGAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
133240008 |
- |
VENTX |
NNNATGGAGAGACCCCGAGA |
NGG |
2 |
0.0991 |
Tier I |
| 3 |
NC_000017.11 |
17 |
45408709 |
- |
ARHGAP27 |
NNNAGGCAGAGAACCAGAGA |
NGG |
2 |
0.64 |
Tier II |
| 4 |
NC_000023.11 |
X |
103083288 |
+ |
NXF3 |
NNNCTGCAGAGAACCCAAGA |
NGG |
2 |
0.3294 |
Tier II |
| 5 |
NC_000001.11 |
1 |
156072083 |
- |
MEX3A |
NNNATGCAGAGAAACCAAGA |
NGG |
2 |
0.3267 |
Tier II |
| 6 |
NC_000011.10 |
11 |
64648998 |
- |
NRXN2 |
NNNACGCAGAGAACCTGAGA |
NGG |
2 |
0.3077 |
Tier II |
| 7 |
NC_000010.11 |
10 |
60658612 |
- |
ANK3 |
NNNATGCAGAGCACCAGAGA |
NGG |
2 |
0.2632 |
Tier II |
| 8 |
NC_000012.12 |
12 |
3834760 |
+ |
PARP11 |
NNNATGCAGAGAACCAGAGA |
NAG |
2 |
0.2593 |
Tier II |
| 9 |
NC_000010.11 |
10 |
75063669 |
- |
DUSP29 |
NNNATGCAGAGCACCCAAGA |
NGG |
2 |
0.2456 |
Tier II |
| 10 |
NC_000009.12 |
9 |
4637263 |
- |
SPATA6L |
NNNATGCAGAGACCCAGAGA |
NGG |
2 |
0.2105 |
Tier II |
| 11 |
NC_000009.12 |
9 |
130449740 |
+ |
ASS1 |
NNNATGAAGAGAACCCCAGA |
NGG |
2 |
0.1765 |
Tier II |
| 12 |
NC_000022.11 |
22 |
43488381 |
+ |
MPPED1 |
NNNAGGCAGAGAACCCGAGC |
NGG |
2 |
0.1455 |
Tier II |
| 13 |
NC_000006.12 |
6 |
61864772 |
- |
KHDRBS2 |
NNNATGCAGAGAACCCCAGT |
NGG |
2 |
0.1412 |
Tier II |
| 14 |
NC_000012.12 |
12 |
111043379 |
+ |
CUX2 |
NNNTTGCAGAGACCCCGAGA |
NGG |
2 |
0.134 |
Tier II |
| 15 |
NC_000017.11 |
17 |
11490236 |
+ |
SHISA6 |
NNNCTGCAGAGTACCCGAGA |
NGG |
2 |
0.1176 |
Tier II |
| 16 |
NC_000014.9 |
14 |
92374111 |
+ |
SLC24A4 |
NNNATGCAGAGAACCCCAGA |
NAG |
2 |
0.061 |
Tier II |
| 17 |
NC_000014.9 |
14 |
56647089 |
- |
TMEM260 |
NNNATGCAGAGAACCCAAGA |
NTG |
2 |
0.0364 |
Tier II |
| 18 |
NC_000010.11 |
10 |
132779565 |
- |
INPP5A |
NNNATCCAGAGAACCCGAGA |
NGC |
2 |
0.0152 |
Tier II |
| 19 |
NC_000006.12 |
6 |
2363884 |
+ |
GMDS-DT |
NNNATGCAGAGAATCCGGGA |
NGG |
2 |
0.2933 |
Tier III |
| 20 |
NC_000022.11 |
22 |
22730577 |
+ |
IGL |
NNNAAGCAGAGAACCCGTGA |
NGG |
2 |
0.25 |
Tier III |
| 21 |
NC_000011.10 |
11 |
46856199 |
+ |
LRP4-AS1 |
NNNATCCAGAGAACCTGAGA |
NGG |
2 |
0.2098 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
58956819 |
+ |
Zkscan16 |
NNNATGCGGTGAACCCGAGA |
NGG |
2 |
0.6471 |
Tier I |
| 2 |
NC_000068.7 |
2 |
157463043 |
+ |
Src |
NNNACGCCGAGAACCCGAGA |
NGG |
2 |
0.4286 |
Tier I |
| 3 |
NC_000068.7 |
2 |
51925674 |
- |
Rbm43 |
NNNATGGAGAGAACCCTAGA |
NGG |
2 |
0.1176 |
Tier I |
| 4 |
NC_000075.6 |
9 |
15323479 |
+ |
Cep295 |
NNNATGCAGAGAACCAGAGA |
NGG |
1 |
1.0 |
Tier II |
| 5 |
NC_000073.6 |
7 |
28426418 |
- |
Samd4b |
NNNTTGCAGAGAACCAGAGA |
NGG |
2 |
0.6364 |
Tier II |
| 6 |
NC_000072.6 |
6 |
54701786 |
+ |
Mturn |
NNNATGCAGGGAACCCAAGA |
NGG |
2 |
0.5185 |
Tier II |
| 7 |
NC_000068.7 |
2 |
3757889 |
- |
Fam107b |
NNNTTGCAGAGGACCCGAGA |
NGG |
2 |
0.4596 |
Tier II |
| 8 |
NC_000078.6 |
12 |
75997215 |
+ |
Syne2 |
NNNTTGCAGAGAACCCGAGT |
NGG |
2 |
0.3818 |
Tier II |
| 9 |
NC_000067.6 |
1 |
87087864 |
+ |
Alppl2 |
NNNCTGCAGAGAACCAGAGA |
NGG |
2 |
0.3529 |
Tier II |
| 10 |
NC_000069.6 |
3 |
88541325 |
+ |
Mex3a |
NNNATGCAGAGAAACCAAGA |
NGG |
2 |
0.3267 |
Tier II |
| 11 |
NC_000074.6 |
8 |
104191725 |
- |
Bean1 |
NNNATGCAGAGAACCCAAGA |
NAG |
2 |
0.242 |
Tier II |
| 12 |
NC_000069.6 |
3 |
118929079 |
- |
Dpyd |
NNNATCCAGAGAACCTGAGA |
NGG |
2 |
0.2098 |
Tier II |
| 13 |
NC_000073.6 |
7 |
113528699 |
- |
Far1 |
NNNTTGCAGAGAACCTGAGA |
NGG |
2 |
0.1958 |
Tier II |
| 14 |
NC_000084.6 |
18 |
22732956 |
+ |
Nol4 |
NNNGTGCAGAGATCCCGAGA |
NGG |
2 |
0.1875 |
Tier II |
| 15 |
NC_000068.7 |
2 |
163534735 |
- |
Hnf4a |
NNNTTGCAGAGAACCCTAGA |
NGG |
2 |
0.1591 |
Tier II |
| 16 |
NC_000083.6 |
17 |
44766597 |
- |
Runx2 |
NNNATGCTGAGAACCTGAGA |
NGG |
2 |
0.1319 |
Tier II |
| 17 |
NC_000068.7 |
2 |
125363353 |
+ |
Fbn1 |
NNNATGCAGACACCCCGAGA |
NGG |
2 |
0.0902 |
Tier II |
| 18 |
NC_000072.6 |
6 |
50228022 |
- |
Gsdme |
NNNATGCAGTGAACCCGAGA |
NGC |
2 |
0.0196 |
Tier II |
| 19 |
NC_000069.6 |
3 |
65743059 |
- |
Lekr1 |
NNNATGCAGAGAACCCCAGA |
NTG |
2 |
0.0092 |
Tier II |
| 20 |
NC_000067.6 |
1 |
139115698 |
- |
Dennd1b |
NNNATGCAGAGAAGCCGAGC |
NGG |
2 |
0.0 |
Tier II |
| 21 |
NC_000082.6 |
16 |
93505883 |
+ |
Gm30695 |
NNNATGCAGAGAACCAGACA |
NGG |
2 |
0.4483 |
Tier III |
| 22 |
NC_000067.6 |
1 |
87087864 |
+ |
Gm29371 |
NNNCTGCAGAGAACCAGAGA |
NGG |
2 |
0.3529 |
Tier III |
| 23 |
NC_000074.6 |
8 |
124080741 |
- |
Gm30005 |
NNNATGCAGTGAACCTGAGA |
NGG |
2 |
0.2715 |
Tier III |
| 24 |
NC_000076.6 |
10 |
91896902 |
+ |
Gm31592 |
NNNATGCAGAGAACCCCAGA |
NGG |
1 |
0.2353 |
Tier III |
| 25 |
NC_000068.7 |
2 |
163534735 |
- |
Hnf4aos |
NNNTTGCAGAGAACCCTAGA |
NGG |
2 |
0.1591 |
Tier III |
Other clones with same target sequence:
(none)
