Construct: sgRNA BRDN0001146191
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGCGTCAAGCACCACACCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MATK (4145)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77012
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
3784208 |
- |
MATK |
NNNGTCAAGCACCACACCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
43016301 |
- |
RIT2 |
NNNATCAAGCACCACACCAA |
NGG |
2 |
0.8438 |
Tier II |
| 3 |
NC_000001.11 |
1 |
65480107 |
+ |
LEPR |
NNNGTCAACCACCACACCAA |
NGG |
2 |
0.5048 |
Tier II |
| 4 |
NC_000016.10 |
16 |
89294751 |
+ |
ANKRD11 |
NNNGTCAGGCACCACACAAG |
NGG |
2 |
0.3949 |
Tier II |
| 5 |
NC_000002.12 |
2 |
9424731 |
+ |
CPSF3 |
NNNGTCAAGCACCACACATG |
NGG |
2 |
0.2899 |
Tier II |
| 6 |
NC_000010.11 |
10 |
24215691 |
- |
KIAA1217 |
NNNGTCAAGCCCCACACCTG |
NGG |
2 |
0.2154 |
Tier II |
| 7 |
NC_000005.10 |
5 |
11090107 |
- |
CTNND2 |
NNNTTCCAGCACCACACCAG |
NGG |
2 |
0.1591 |
Tier II |
| 8 |
NC_000011.10 |
11 |
131629966 |
- |
NTM |
NNNGTCAAGCATCACACCAG |
NGA |
2 |
0.0374 |
Tier II |
| 9 |
NC_000002.12 |
2 |
215069327 |
+ |
ABCA12 |
NNNGTCAAGCACCACAACAG |
NGA |
2 |
0.0324 |
Tier II |
| 10 |
NC_000005.10 |
5 |
64844121 |
+ |
CWC27 |
NNNGTCAAGCACAACACCAG |
NTG |
2 |
0.015 |
Tier II |
| 11 |
NC_000020.11 |
20 |
62426267 |
+ |
RBBP8NL |
NNNGTCAAGCACCACACCAG |
NCT |
2 |
0.0 |
Tier II |
| 12 |
NC_000001.11 |
1 |
30193990 |
+ |
LOC105378617 |
NNNGCCAGGCACCACACCAG |
NGG |
2 |
0.7333 |
Tier III |
| 13 |
NC_000001.11 |
1 |
30195717 |
- |
LOC105378617 |
NNNGCCAGGCACCACACCAG |
NGG |
2 |
0.7333 |
Tier III |
| 14 |
NC_000006.12 |
6 |
11493153 |
- |
LOC105374928 |
NNNGTCAAACACAACACCAG |
NGG |
2 |
0.2473 |
Tier III |
| 15 |
NC_000014.9 |
14 |
53617095 |
+ |
LOC105370504 |
NNNGTCAAGAACCACCCCAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
66715172 |
- |
Tg |
NNNGTCAAGCACTGCACCAG |
NGG |
2 |
0.3267 |
Tier I |
| 2 |
NC_000068.7 |
2 |
97628716 |
+ |
Lrrc4c |
NNNGGTAAGCACCACACCAG |
NGG |
2 |
0.5943 |
Tier II |
| 3 |
NC_000076.6 |
10 |
119472202 |
- |
Grip1 |
NNNGGCAAGCACCACAACAG |
NGG |
2 |
0.2987 |
Tier II |
| 4 |
NC_000082.6 |
16 |
10625960 |
+ |
Clec16a |
NNNCTCAAGCACCACATCAG |
NGG |
2 |
0.2471 |
Tier II |
| 5 |
NC_000067.6 |
1 |
171534541 |
+ |
Itln1 |
NNNGTCCAGCACCACACAAG |
NGG |
2 |
0.2356 |
Tier II |
| 6 |
NC_000077.6 |
11 |
118624825 |
- |
Rbfox3 |
NNNGTCAAGCATCACACCAG |
NGT |
2 |
0.0087 |
Tier II |
| 7 |
NC_000068.7 |
2 |
69696438 |
+ |
Fastkd1 |
NNNGGCAAGCACCACCCCAG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000078.6 |
12 |
33060202 |
+ |
Cdhr3 |
NNNGTCAAGCACCACCCAAG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000070.6 |
4 |
130112995 |
- |
Pef1 |
NNNGTCAAGCACCACTCCAG |
NTG |
2 |
0.0 |
Tier II |
| 10 |
NC_000078.6 |
12 |
16233599 |
+ |
Gm48606 |
NNNGACAAGCACCACAGCAG |
NGG |
2 |
0.0294 |
Tier III |
Other clones with same target sequence:
(none)
