Construct: sgRNA BRDN0001146192

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCAAGATCTTTCATCTCTGG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACVR1C (130399)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76525

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 157550258 + ACVR1C NNNAGATCTTTCATCTCTGG NGG 0 1.0 Tier I
2 NC_000004.12 4 75981429 + SDAD1 NNNAGATCTTTCACCTCTTG NGG 2 0.1905 Tier I
3 NC_000015.10 15 42753176 + TTBK2 NNNAGATCTTTCTTCTCTGG NTG 2 0.0117 Tier I
4 NC_000001.11 1 39316504 + MACF1 NNNAGATCTCTCAGCTCTGG NGG 2 0.0 Tier I
5 NC_000004.12 4 150100810 - DCLK2 NNNAGATTTTTCATCTCTGT NGG 2 0.6125 Tier II
6 NC_000001.11 1 214502189 + PTPN14 NNNAGATTTTTCATCCCTGG NGG 2 0.5833 Tier II
7 NC_000022.11 22 37353807 + ELFN2 NNNGGAACTTTCATCTCTGG NGG 2 0.5469 Tier II
8 NC_000002.12 2 127592650 + MYO7B NNNAGTTCTTCCATCTCTGG NGG 2 0.4762 Tier II
9 NC_000003.12 3 151558780 + IGSF10 NNNAGATTTGTCATCTCTGG NGG 2 0.4375 Tier II
10 NC_000020.11 20 34958433 + MYH7B NNNAGATGTTTCATCCCTGG NGG 2 0.4286 Tier II
11 NC_000001.11 1 20033400 + PLA2G5 NNNAGAGCTTTAATCTCTGG NGG 2 0.4202 Tier II
12 NC_000003.12 3 170512132 - SLC7A14 NNNAGATCTGTCATCTCTGT NGG 2 0.35 Tier II
13 NC_000003.12 3 67545365 - SUCLG2 NNNAGATCTTTCATCTCTCT NGG 2 0.3138 Tier II
14 NC_000008.11 8 9696746 - TNKS NNNAGATCTTTCATCTCTCT NGG 2 0.3138 Tier II
15 NC_000003.12 3 38524683 - EXOG NNNAGATCTTTCATCTTTTG NGG 2 0.3111 Tier II
16 NC_000004.12 4 150142365 + DCLK2 NNNAGATCTTTCTTCTCTGA NGG 2 0.2813 Tier II
17 NC_000016.10 16 58615120 - CNOT1 NNNAGATATTTCATCTCTGC NGG 2 0.2786 Tier II
18 NC_000023.11 X 72570181 - HDAC8 NNNAGATTTTTCACCTCTGG NGG 2 0.25 Tier II
19 NC_000009.12 9 34811573 - PHF24 NNNATATCTTACATCTCTGG NGG 2 0.225 Tier II
20 NC_000009.12 9 95457236 + PTCH1 NNNTGATCTTTCTTCTCTGG NGG 2 0.1909 Tier II
21 NC_000001.11 1 54830102 + CIMAP2 NNNAGAACTTTCCTCTCTGG NGG 2 0.1842 Tier II
22 NC_000012.12 12 114407533 - TBX5 NNNAGATTTTTCCTCTCTGG NGG 2 0.1842 Tier II
23 NC_000012.12 12 31715663 + AMN1 NNNAGATCTTTCTTCTTTGG NGG 2 0.14 Tier II
24 NC_000023.11 X 7288623 + STS NNNAGATATTTCATCTGTGG NGG 2 0.0382 Tier II
25 NC_000010.11 10 48252133 + FRMPD2 NNNACATCTTTCATCTCTGG NTG 2 0.0306 Tier II
26 NC_000019.10 19 40412364 + PRX NNNAGATCCTTCATCTCTGG NGC 2 0.0205 Tier II
27 NC_000004.12 4 86461788 + MAPK10 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier II
28 NC_000004.12 4 91186154 - CCSER1 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier II
29 NC_000006.12 6 45236596 + SUPT3H NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier II
30 NC_000016.10 16 48354742 - LONP2 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier II
31 NC_000017.11 17 29990884 - EFCAB5 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier II
32 NC_000019.10 19 12022870 + ZNF433 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier II
33 NC_000020.11 20 5304113 + PROKR2 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier II
34 NC_000001.11 1 116950998 + PTGFRN NNNAGATTTTTCATCTCTGG NGC 2 0.0194 Tier II
35 NC_000009.12 9 83533150 - FRMD3 NNNAGCTCTTTCATCTCTGG NGC 2 0.0101 Tier II
36 NC_000003.12 3 120600946 - NDUFB4 NNNAGATCTTTCATTTCTGG NGT 2 0.0011 Tier II
37 NC_000015.10 15 52099420 + LOC112268150 NNNACATTTTTCATCTCTGG NGG 2 0.6875 Tier III
38 NC_000022.11 22 37353807 + LOC100506271 NNNGGAACTTTCATCTCTGG NGG 2 0.5469 Tier III
39 NC_000003.12 3 165381383 - LINC01322 NNNAGATCTTTCATCTCAAG NGG 2 0.4762 Tier III
40 NC_000003.12 3 151558780 + LINC02066 NNNAGATTTGTCATCTCTGG NGG 2 0.4375 Tier III
41 NC_000003.12 3 170512132 - SLC7A14-AS1 NNNAGATCTGTCATCTCTGT NGG 2 0.35 Tier III
42 NC_000003.12 3 104286608 - LOC124909492 NNNAGATCTTTCTTCTCTAG NGG 2 0.2143 Tier III
43 NC_000004.12 4 75981429 + SDAD1-AS1 NNNAGATCTTTCACCTCTTG NGG 2 0.1905 Tier III
44 NC_000012.12 12 31715663 + STMN1P1 NNNAGATCTTTCTTCTTTGG NGG 2 0.14 Tier III
45 NC_000012.12 12 132122104 - EP400P1 NNNAGATCTGTCCTCTCTGG NGG 2 0.1053 Tier III
46 NC_000014.9 14 26794047 - LINC02294 NNNAGATATTTCATCTCTGG NTG 2 0.0253 Tier III
47 NC_000014.9 14 26794047 - NOVA1-DT NNNAGATATTTCATCTCTGG NTG 2 0.0253 Tier III
48 NC_000001.11 1 247025517 + ZNF670-ZNF695 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier III
49 NC_000002.12 2 33918636 - LINC01317 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier III
50 NC_000004.12 4 127276603 - LOC102724210 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier III
51 NC_000004.12 4 127276603 - LOC107986312 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier III
52 NC_000005.10 5 121860087 - LOC105379149 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier III
53 NC_000007.14 7 155243313 - LOC105375587 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier III
54 NC_000009.12 9 122620544 - LOC124902265 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier III
55 NC_000012.12 12 105308374 - C12orf75-AS1 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier III
56 NC_000015.10 15 25637174 + LOC124903448 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier III
57 NC_000019.10 19 12022870 + ZNF433-AS1 NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier III
58 NC_000019.10 19 12103411 - ZNF788P NNNAGATCTTTCTTTTCTGG NGG 2 0.02 Tier III
59 NC_000013.11 13 21372131 + MIPEPP3 NNNAGATCTTTCCTTTCTGG NGG 2 0.014 Tier III
60 NC_000013.11 13 100722977 - NALCN-AS1 NNNAGATCTGTCATCTCTGG NGC 2 0.0111 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 58287777 + Acvr1c NNNAGATCTCTCATCCCTGG NGG 2 0.3556 Tier I
2 NC_000067.6 1 80530276 + Dock10 NNNAGATCTTTCACCTCTGC NGG 2 0.1224 Tier I
3 NC_000080.6 14 72584812 + Fndc3a NNNAGATCTTTCATCTCTGA NGT 2 0.0151 Tier I
4 NC_000068.7 2 120746218 + Ttbk2 NNNAGATCTTTCTTCTCTGG NTG 2 0.0117 Tier I
5 NC_000068.7 2 141497758 - Macrod2 NNNAGAACTTTCATCTCTAG NGG 2 0.625 Tier II
6 NC_000081.6 15 32575403 - Sema5a NNNAGTTCTTACATCTCTGG NGG 2 0.5357 Tier II
7 NC_000069.6 3 51304492 - Elf2 NNNTGTTCTTTCATCTCTGG NGG 2 0.4545 Tier II
8 NC_000084.6 18 80320771 + Kcng2 NNNAGATCTGTCATCTCTAG NGG 2 0.3571 Tier II
9 NC_000073.6 7 45924996 + Ccdc114 NNNACATCTTTCATCTCTGC NGG 2 0.3367 Tier II
10 NC_000070.6 4 152084181 - Plekhg5 NNNAGATGTGTCATCTCTGG NGG 2 0.3214 Tier II
11 NC_000081.6 15 34620142 + Nipal2 NNNAGATCTTTCATCTCCTG NGG 2 0.2857 Tier II
12 NC_000072.6 6 113683720 + Irak2 NNNAGATCTTTCTTCCCTGG NGG 2 0.2 Tier II
13 NC_000083.6 17 68718657 + L3mbtl4 NNNATATCTTCCATCTCTGG NGG 2 0.2 Tier II
14 NC_000075.6 9 115933630 - Gadl1 NNNAGATCTTTCCTCTCAGG NGG 2 0.1404 Tier II
15 NC_000085.6 19 7131732 + Macrod1 NNNAGATCTTTCACATCTGG NGG 2 0.0635 Tier II
16 NC_000085.6 19 7131732 + Flrt1 NNNAGATCTTTCACATCTGG NGG 2 0.0635 Tier II
17 NC_000072.6 6 110666971 + Grm7 NNNAGACCTTTCATCTGTGG NGG 2 0.0404 Tier II
18 NC_000083.6 17 26697201 + Atp6v0e NNNAGATCTTTCATCTCTAG NTG 2 0.0278 Tier II
19 NC_000074.6 8 16019197 - Csmd1 NNNAGATGTTTCATCTCTGG NTG 2 0.025 Tier II
20 NC_000076.6 10 86352488 - Syn3 NNNAGATTTTTCATCTCTGG NGC 2 0.0194 Tier II
21 NC_000076.6 10 89153716 + Ano4 NNNAAATCTTTCATCTCTGG NGC 2 0.0193 Tier II
22 NC_000079.6 13 54303813 - Cplx2 NNNAGATCTTTCTTCTCTGG NTG 2 0.0117 Tier II
23 NC_000073.6 7 109507545 - Trim66 NNNAGACCTTTCATCTCTGG NGT 2 0.0111 Tier II
24 NC_000082.6 16 16436493 - Fgd4 NNNAGATCTTTCATCTCTTG NGT 2 0.0108 Tier II
25 NC_000077.6 11 101028086 + Atp6v0a1 NNNAAATCTTTCAGCTCTGG NGG 2 0.0 Tier II
26 NC_000084.6 18 61471361 - Lncbate10 NNNAGGTCTTTCATCTCTAG NGG 2 0.5102 Tier III
27 NC_000068.7 2 78814119 - Gm39865 NNNAGTTGTTTCATCTCTGG NGG 2 0.4592 Tier III
28 NC_000069.6 3 45811022 + Gm32340 NNNAGATCTTTCATCCCTTG NGG 2 0.4444 Tier III
29 NC_000084.6 18 80320771 + Gm29913 NNNAGATCTGTCATCTCTAG NGG 2 0.3571 Tier III
30 NC_000077.6 11 33625134 - D130052B06Rik NNNAGATATGTCATCTCTGG NGG 2 0.325 Tier III
31 NC_000071.6 5 50153427 + 4930448I18Rik NNNCCATCTTTCATCTCTGG NGG 2 0.2773 Tier III
32 NC_000073.6 7 95106799 + Gm32647 NNNATATGTTTCATCTCTGG NGG 2 0.1929 Tier III
33 NC_000085.6 19 7131732 + Gm38621 NNNAGATCTTTCACATCTGG NGG 2 0.0635 Tier III
34 NC_000079.6 13 54303813 - LOC115488208 NNNAGATCTTTCTTCTCTGG NTG 2 0.0117 Tier III
35 NC_000075.6 9 91928527 + Gm31409 NNNAGATCTTTCAGCACTGG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)