Construct: sgRNA BRDN0001146193
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCGCTGCCAGAAGATCCGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NEK2 (4751)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76214
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
211675405 |
- |
NEK2 |
NNNGCTGCCAGAAGATCCGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
27238338 |
- |
NSMCE1 |
NNNGCTGCCAGAGGATCAGG |
NGG |
2 |
0.3512 |
Tier II |
| 3 |
NC_000006.12 |
6 |
38246109 |
- |
BTBD9 |
NNNGCTGCCAGCAGATCCTG |
NGG |
2 |
0.1754 |
Tier II |
| 4 |
NC_000018.10 |
18 |
3450280 |
+ |
TGIF1 |
NNNGCTGCCAGAAGATCCCG |
NCG |
2 |
0.048 |
Tier II |
| 5 |
NC_000017.11 |
17 |
42783833 |
+ |
WNK4 |
NNNGCTGGCAGAAGATGCGG |
NGG |
2 |
0.0378 |
Tier II |
| 6 |
NC_000011.10 |
11 |
1486781 |
+ |
MOB2 |
NNNGCTGCCAGAAGAGGCGG |
NGG |
2 |
0.0204 |
Tier II |
| 7 |
NC_000014.9 |
14 |
18890350 |
- |
NEK2P1 |
NNNGCTGCCAGAAGATCCGG |
NGG |
0 |
1.0 |
Tier III |
| 8 |
NC_000002.12 |
2 |
131178872 |
- |
NEK2P4 |
NNNGCTGCCAGAAGATCCAG |
NGG |
1 |
0.7143 |
Tier III |
| 9 |
NC_000014.9 |
14 |
18699904 |
- |
NEK2P3 |
NNNGCTGCCAGAAGATCCAG |
NGG |
1 |
0.7143 |
Tier III |
| 10 |
NC_000022.11 |
22 |
15613021 |
- |
NEK2P2 |
NNNGCTGCCAGAAGATCCAG |
NGG |
1 |
0.7143 |
Tier III |
| 11 |
NC_000014.9 |
14 |
19629081 |
+ |
LOC107984133 |
NNNGCTGCCGGAAGATCCGG |
NGG |
1 |
0.5556 |
Tier III |
| 12 |
NC_000006.12 |
6 |
38246109 |
- |
LOC105375045 |
NNNGCTGCCAGCAGATCCTG |
NGG |
2 |
0.1754 |
Tier III |
| 13 |
NC_000018.10 |
18 |
3450280 |
+ |
LOC124904237 |
NNNGCTGCCAGAAGATCCCG |
NCG |
2 |
0.048 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
191821721 |
+ |
Nek2 |
NNNGCTGTCAGAAGATTCGG |
NGG |
2 |
0.4083 |
Tier I |
| 2 |
NC_000082.6 |
16 |
38371205 |
- |
Popdc2 |
NNNGCAGCCAGAAGATCTGG |
NGG |
2 |
0.5571 |
Tier II |
| 3 |
NC_000074.6 |
8 |
15062113 |
- |
Myom2 |
NNNGCTGACAGAAGATCCTG |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000067.6 |
1 |
166125583 |
+ |
Dusp27 |
NNNGCTGCCTGAAGATCCGG |
NTG |
2 |
0.0344 |
Tier II |
| 5 |
NC_000084.6 |
18 |
68180345 |
+ |
Ldlrad4 |
NNNGCTGCCAGAAGATCCTG |
NTG |
2 |
0.026 |
Tier II |
| 6 |
NC_000083.6 |
17 |
74078307 |
- |
Gm31235 |
NNNGCTGCCTGAAGATCAGG |
NGG |
2 |
0.4751 |
Tier III |
Other clones with same target sequence:
(none)
