Construct: sgRNA BRDN0001146198
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGGACATACTGGACGCTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK32C (282974)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76931
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
132224460 |
+ |
STK32C |
NNNGACATACTGGACGCTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
227618038 |
- |
ZNF678 |
NNNGACAGACTGGACACTCA |
NGG |
2 |
0.7333 |
Tier II |
| 3 |
NC_000002.12 |
2 |
212210069 |
+ |
ERBB4 |
NNNGACATACTGGACACTCT |
NGG |
2 |
0.6 |
Tier II |
| 4 |
NC_000007.14 |
7 |
2085891 |
+ |
MAD1L1 |
NNNGACCCACTGGACGCTCA |
NGG |
2 |
0.4375 |
Tier II |
| 5 |
NC_000001.11 |
1 |
18261719 |
- |
IGSF21 |
NNNGACATTCTGGACGCTGA |
NGG |
2 |
0.075 |
Tier II |
| 6 |
NC_000003.12 |
3 |
42597174 |
+ |
SEC22C |
NNNGACACACTGGACTCTCA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000018.10 |
18 |
51530522 |
- |
LINC01630 |
NNNGACATAATGCACGCTCA |
NGG |
2 |
0.3649 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
181028554 |
+ |
Chrna4 |
NNNCACATGCTGGACGCTCA |
NGG |
2 |
0.3529 |
Tier I |
| 2 |
NC_000082.6 |
16 |
3979918 |
- |
Slx4 |
NNNGACATCCTGGACGCTCA |
NTG |
2 |
0.0223 |
Tier I |
| 3 |
NC_000086.7 |
X |
141288517 |
- |
Col4a6 |
NNNGATACACTGGACGCTCA |
NGG |
2 |
0.9286 |
Tier II |
| 4 |
NC_000078.6 |
12 |
66642913 |
- |
Mdga2 |
NNNGACATTCTGGACACTCA |
NGG |
2 |
0.6 |
Tier II |
| 5 |
NC_000083.6 |
17 |
47371041 |
+ |
Mrps10 |
NNNGACATAGCGGACGCTCA |
NGG |
2 |
0.2593 |
Tier II |
| 6 |
NC_000069.6 |
3 |
79435391 |
+ |
Gm17359 |
NNNGACCTCCTGGACGCTCA |
NGG |
2 |
0.25 |
Tier II |
Other clones with same target sequence:
(none)
