Construct: sgRNA BRDN0001146202
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGTGTTGCAATAATCTCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKG2 (5593)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76921
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
81142840 |
+ |
PRKG2 |
NNNGTGTTGCAATAATCTCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
32524605 |
- |
AKAP6 |
NNNGTGTTGTAATAATCTCT |
NGG |
2 |
0.5647 |
Tier II |
| 3 |
NC_000003.12 |
3 |
41201484 |
- |
CTNNB1 |
NNNCTGTTGCAATAATCTCA |
NGG |
1 |
0.5294 |
Tier II |
| 4 |
NC_000016.10 |
16 |
76533041 |
- |
CNTNAP4 |
NNNGTGTTGCAATAAACTTA |
NGG |
2 |
0.4196 |
Tier II |
| 5 |
NC_000009.12 |
9 |
4139032 |
+ |
GLIS3 |
NNNATGTTGCCATAATCTCA |
NGG |
2 |
0.36 |
Tier II |
| 6 |
NC_000002.12 |
2 |
102629960 |
+ |
SLC9A2 |
NNNGTGTTACAATAATTTCA |
NGG |
2 |
0.3 |
Tier II |
| 7 |
NC_000004.12 |
4 |
101097106 |
- |
PPP3CA |
NNNGTGATGCACTAATCTCA |
NGG |
2 |
0.2303 |
Tier II |
| 8 |
NC_000003.12 |
3 |
47245954 |
+ |
KIF9 |
NNNGTGTTGCAATATTCACA |
NGG |
2 |
0.1333 |
Tier II |
| 9 |
NC_000014.9 |
14 |
32954091 |
- |
NPAS3 |
NNNGTGTTGCATTAAGCTCA |
NGG |
2 |
0.1154 |
Tier II |
| 10 |
NC_000018.10 |
18 |
72818002 |
- |
NETO1 |
NNNGAGTTGCAATCATCTCA |
NGG |
2 |
0.1071 |
Tier II |
| 11 |
NC_000019.10 |
19 |
8494435 |
+ |
PRAM1 |
NNNGTGTTGCAAGAAGCTCA |
NGG |
2 |
0.0903 |
Tier II |
| 12 |
NC_000023.11 |
X |
154715308 |
+ |
GAB3 |
NNNATGTTGCAATAATGTCA |
NGG |
2 |
0.0529 |
Tier II |
| 13 |
NC_000023.11 |
X |
140783250 |
+ |
LINC00632 |
NNNGTCTTCCAATAATCTCA |
NGG |
2 |
0.3671 |
Tier III |
| 14 |
NC_000005.10 |
5 |
124369933 |
- |
LINC01170 |
NNNGTGTTGCAGTAAGCTCA |
NGG |
2 |
0.25 |
Tier III |
| 15 |
NC_000011.10 |
11 |
22166222 |
- |
LOC124902645 |
NNNGTGTTGCAATTATATCA |
NGG |
2 |
0.2489 |
Tier III |
| 16 |
NC_000014.9 |
14 |
30558497 |
+ |
G2E3-AS1 |
NNNTTGTTTCAATAATCTCA |
NGG |
2 |
0.1939 |
Tier III |
| 17 |
NC_000001.11 |
1 |
71159770 |
+ |
ZRANB2-DT |
NNNGTGTTGCAATATTTTCA |
NGG |
2 |
0.0933 |
Tier III |
| 18 |
NC_000009.12 |
9 |
25544365 |
+ |
LOC105375996 |
NNNGTGTTGCAATAATCTCA |
NTT |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
98971898 |
+ |
Prkg2 |
NNNGTGTTGCGATAATCTCG |
NGG |
2 |
0.4971 |
Tier I |
| 2 |
NC_000081.6 |
15 |
8340043 |
- |
Nipbl |
NNNCTGTTGTAATAATCTCA |
NGG |
2 |
0.4983 |
Tier II |
| 3 |
NC_000073.6 |
7 |
143487643 |
+ |
Slc22a18 |
NNNGGGGTGCAATAATCTCA |
NGG |
2 |
0.3765 |
Tier II |
| 4 |
NC_000074.6 |
8 |
80014612 |
+ |
Hhip |
NNNGGGTTGCAATAATCTTA |
NGG |
2 |
0.2954 |
Tier II |
| 5 |
NC_000076.6 |
10 |
12470054 |
- |
Utrn |
NNNTTGTTACAATAATCTCA |
NGG |
2 |
0.2338 |
Tier II |
| 6 |
NC_000086.7 |
X |
145336229 |
+ |
Lhfpl1 |
NNNGTGTTGAAATAATCTCC |
NGG |
2 |
0.197 |
Tier II |
| 7 |
NC_000084.6 |
18 |
67672505 |
- |
Ptpn2 |
NNNGTGGTGCATTAATCTCA |
NGG |
2 |
0.1961 |
Tier II |
| 8 |
NC_000074.6 |
8 |
35801789 |
+ |
Gm19410 |
NNNGTGTTGCAATAACCTCA |
NTG |
2 |
0.026 |
Tier II |
| 9 |
NC_000078.6 |
12 |
77574023 |
- |
Gm35240 |
NNNGTGTTGCCATAATCTCC |
NGG |
2 |
0.0909 |
Tier III |
Other clones with same target sequence:
(none)
