Construct: sgRNA BRDN0001146205
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGACTGAGCCATTACTCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MERTK (10461)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76446
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
111975310 |
- |
MERTK |
NNNACTGAGCCATTACTCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000011.10 |
11 |
122656046 |
- |
UBASH3B |
NNNACTGAGCCATGGCTCAG |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000009.12 |
9 |
96766088 |
- |
ZNF510 |
NNNACTGAACCATTACTCAG |
NGG |
1 |
0.6429 |
Tier II |
| 4 |
NC_000003.12 |
3 |
33378026 |
+ |
FBXL2 |
NNNACTGGGCCAATACTCAG |
NGG |
2 |
0.5077 |
Tier II |
| 5 |
NC_000009.12 |
9 |
4491593 |
+ |
SLC1A1 |
NNNTCTGAGCCATTGCTCAG |
NGG |
2 |
0.4136 |
Tier II |
| 6 |
NC_000019.10 |
19 |
7386745 |
+ |
ARHGEF18 |
NNNTCTGAGCCATTGCTCAG |
NGG |
2 |
0.4136 |
Tier II |
| 7 |
NC_000005.10 |
5 |
156009777 |
- |
SGCD |
NNNAGTGAGCCATAACTCAG |
NGG |
2 |
0.3714 |
Tier II |
| 8 |
NC_000022.11 |
22 |
45358409 |
+ |
SMC1B |
NNNACTGAGCCATTACTTTG |
NGG |
2 |
0.3462 |
Tier II |
| 9 |
NC_000020.11 |
20 |
64178935 |
+ |
MYT1 |
NNNACTGAGCCGTTATTCAG |
NGG |
2 |
0.2222 |
Tier II |
| 10 |
NC_000020.11 |
20 |
64179058 |
+ |
MYT1 |
NNNACTGAGCCGTTATTCAG |
NGG |
2 |
0.2222 |
Tier II |
| 11 |
NC_000020.11 |
20 |
64179222 |
+ |
MYT1 |
NNNACTGAGCCATTATTCGG |
NGG |
2 |
0.1154 |
Tier II |
| 12 |
NC_000019.10 |
19 |
43265439 |
- |
PSG9 |
NNNACTGAGCCATTAGTCAT |
NGG |
2 |
0.1077 |
Tier II |
| 13 |
NC_000006.12 |
6 |
24407846 |
- |
MRS2 |
NNNACTGAGCGATCACTCAG |
NGG |
2 |
0.0714 |
Tier II |
| 14 |
NC_000014.9 |
14 |
59848067 |
- |
RTN1 |
NNNTCTGAGCCATTACTCAG |
NTG |
2 |
0.0248 |
Tier II |
| 15 |
NC_000022.11 |
22 |
25065400 |
+ |
KIAA1671 |
NNNACTGAGCCATTCCTCAG |
NTG |
2 |
0.0106 |
Tier II |
| 16 |
NC_000015.10 |
15 |
77034497 |
- |
PSTPIP1 |
NNNGCTGAGCCATGACTCAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000002.12 |
2 |
198558679 |
- |
LOC105373831 |
NNNACTGAGACATTACTCAA |
NGG |
2 |
0.8125 |
Tier III |
| 18 |
NC_000004.12 |
4 |
19514216 |
+ |
LOC105374511 |
NNNAGTGTGCCATTACTCAG |
NGG |
2 |
0.2571 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
128759064 |
- |
Mertk |
NNNACTGAGCCATTACTCAG |
NCG |
1 |
0.1071 |
Tier I |
| 2 |
NC_000082.6 |
16 |
11704404 |
- |
Snx29 |
NNNACTGAGACATTACTCAA |
NGG |
2 |
0.8125 |
Tier II |
| 3 |
NC_000076.6 |
10 |
4284482 |
- |
Akap12 |
NNNACTGAGCCATTGCTCAA |
NGG |
2 |
0.6094 |
Tier II |
| 4 |
NC_000082.6 |
16 |
90433461 |
+ |
Hunk |
NNNATTGAGACATTACTCAG |
NGG |
2 |
0.5515 |
Tier II |
| 5 |
NC_000073.6 |
7 |
6856833 |
- |
Usp29 |
NNNACTGAACCATTACTCTG |
NGG |
2 |
0.3462 |
Tier II |
| 6 |
NC_000086.7 |
X |
144344179 |
- |
Alg13 |
NNNACTGTGCCACTACTCAG |
NGG |
2 |
0.3383 |
Tier II |
| 7 |
NC_000073.6 |
7 |
28721389 |
+ |
Fbxo17 |
NNNAGTGAGCCATTCCTCAG |
NGG |
2 |
0.1636 |
Tier II |
| 8 |
NC_000080.6 |
14 |
10022532 |
- |
Fhit |
NNNACTGAGCCCTTACTCTG |
NGG |
2 |
0.1417 |
Tier II |
| 9 |
NC_000067.6 |
1 |
151434753 |
+ |
Trmt1l |
NNNACTGAGGCTTTACTCAG |
NGG |
2 |
0.1296 |
Tier II |
| 10 |
NC_000082.6 |
16 |
29485336 |
+ |
Atp13a4 |
NNNACTTAGCCATTTCTCAG |
NGG |
2 |
0.1143 |
Tier II |
| 11 |
NC_000069.6 |
3 |
55525442 |
- |
Dclk1 |
NNNCCTGAGCCATCACTCAG |
NGG |
2 |
0.1008 |
Tier II |
| 12 |
NC_000071.6 |
5 |
75030156 |
+ |
Chic2 |
NNNACTGAGCCATCTCTCAG |
NGG |
2 |
0.0571 |
Tier II |
| 13 |
NC_000069.6 |
3 |
85698431 |
+ |
Fam160a1 |
NNNACTGAGCCAGTTCTCAG |
NGG |
2 |
0.0522 |
Tier II |
| 14 |
NC_000071.6 |
5 |
131168919 |
- |
Galnt17 |
NNNAGTGAGCCATTACTCAG |
NGT |
2 |
0.0097 |
Tier II |
| 15 |
NC_000067.6 |
1 |
160112853 |
+ |
Tnn |
NNNACTGAGCCATTAGTCAG |
NTG |
2 |
0.006 |
Tier II |
| 16 |
NC_000079.6 |
13 |
63350370 |
+ |
Fancc |
NNNACTGAGCCATGACTCAT |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000072.6 |
6 |
54258664 |
+ |
Chn2 |
NNNACTGAGCCATTACTCAG |
NCA |
2 |
0.0 |
Tier II |
| 18 |
NC_000076.6 |
10 |
4284482 |
- |
Gm40592 |
NNNACTGAGCCATTGCTCAA |
NGG |
2 |
0.6094 |
Tier III |
| 19 |
NC_000085.6 |
19 |
43639210 |
- |
Gm4311 |
NNNACGGAGCCATTGCTCAG |
NGG |
2 |
0.3714 |
Tier III |
| 20 |
NC_000085.6 |
19 |
43639210 |
- |
Gm34922 |
NNNACGGAGCCATTGCTCAG |
NGG |
2 |
0.3714 |
Tier III |
Other clones with same target sequence:
(none)
