Construct: sgRNA BRDN0001146211
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATTTGAAGGACAAACCCCT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KA5 (9252)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77371
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
90900195 |
- |
RPS6KA5 |
NNNTTGAAGGACAAACCCCT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
119544478 |
- |
PDE5A |
NNNTTGAAGGACATAACCCT |
NGG |
2 |
0.5333 |
Tier II |
| 3 |
NC_000015.10 |
15 |
100194778 |
+ |
ADAMTS17 |
NNNTTGAAGGAGAAAACCCT |
NGG |
2 |
0.4444 |
Tier II |
| 4 |
NC_000007.14 |
7 |
90765050 |
+ |
CDK14 |
NNNTTGAAAGACAAGCCCCT |
NGG |
2 |
0.4179 |
Tier II |
| 5 |
NC_000019.10 |
19 |
19225574 |
+ |
NCAN |
NNNTTGAAAGACAAGCCCCT |
NGG |
2 |
0.4179 |
Tier II |
| 6 |
NC_000016.10 |
16 |
72223932 |
+ |
PMFBP1 |
NNNTTGAAGGGCAAACACCT |
NGG |
2 |
0.3033 |
Tier II |
| 7 |
NC_000004.12 |
4 |
48118650 |
- |
TXK |
NNNTTGCAGCACAAACCCCT |
NGG |
2 |
0.175 |
Tier II |
| 8 |
NC_000001.11 |
1 |
36921880 |
+ |
GRIK3 |
NNNTTGTAGGACAAATCCCT |
NGG |
2 |
0.1346 |
Tier II |
| 9 |
NC_000009.12 |
9 |
5981265 |
- |
BRD10 |
NNNTTGAAGGACAAAGCCCA |
NGG |
2 |
0.0865 |
Tier II |
| 10 |
NC_000003.12 |
3 |
98787690 |
- |
ST3GAL6 |
NNNTTGGAGGACAAACCCCT |
NGA |
2 |
0.049 |
Tier II |
| 11 |
NC_000002.12 |
2 |
61906102 |
- |
COMMD1 |
NNNTTGAAGGAAAAACCCCT |
NTG |
2 |
0.0278 |
Tier II |
| 12 |
NC_000012.12 |
12 |
61897154 |
+ |
TAFA2 |
NNNTTGAAAGACAAACCCCT |
NGT |
2 |
0.0104 |
Tier II |
| 13 |
NC_000005.10 |
5 |
14972140 |
- |
LOC107986376 |
NNNTTGAAGGAGAAACACCT |
NGG |
2 |
0.2074 |
Tier III |
| 14 |
NC_000002.12 |
2 |
68269103 |
+ |
LOC107985892 |
NNNTTGAAGGACAAATCCCT |
NAG |
2 |
0.0798 |
Tier III |
| 15 |
NC_000002.12 |
2 |
47984462 |
+ |
LOC105374591 |
NNNTTGAAGGACACACCCCT |
NGC |
2 |
0.0048 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
131140038 |
+ |
Hspa12b |
NNNTTGAGGAACAAACCCCT |
NGG |
2 |
0.6844 |
Tier II |
| 2 |
NC_000067.6 |
1 |
93996611 |
- |
Gal3st2c |
NNNCTGAAGGGCAAACCCCT |
NGG |
2 |
0.4206 |
Tier II |
| 3 |
NC_000069.6 |
3 |
138748638 |
- |
Tspan5 |
NNNTTGAAGGATAAACCCCA |
NGG |
2 |
0.3029 |
Tier II |
| 4 |
NC_000074.6 |
8 |
25669250 |
- |
Nsd3 |
NNNTAGAAGGACAAACTCCT |
NGG |
2 |
0.2333 |
Tier II |
| 5 |
NC_000080.6 |
14 |
31125333 |
+ |
Smim4 |
NNNGTGAAGGACCAACCCCT |
NGG |
2 |
0.1316 |
Tier II |
| 6 |
NC_000073.6 |
7 |
46166806 |
- |
Abcc8 |
NNNTAGAAGGACAAACCCCT |
NAG |
2 |
0.1296 |
Tier II |
| 7 |
NC_000072.6 |
6 |
144289198 |
+ |
Sox5 |
NNNTTGAAGGACCAACCCAT |
NGG |
2 |
0.0902 |
Tier II |
| 8 |
NC_000077.6 |
11 |
52053512 |
- |
Cdkl3 |
NNNTTGAAGGACAAAGACCT |
NGG |
2 |
0.0718 |
Tier II |
| 9 |
NC_000084.6 |
18 |
15026316 |
- |
Kctd1 |
NNNTTGAAGGACAAAACCCT |
NTG |
2 |
0.039 |
Tier II |
| 10 |
NC_000077.6 |
11 |
83576684 |
+ |
Ccl9 |
NNNTTGAAGCACAAACCCCT |
NTG |
2 |
0.0156 |
Tier II |
| 11 |
NC_000084.6 |
18 |
15026316 |
- |
4933424G05Rik |
NNNTTGAAGGACAAAACCCT |
NTG |
2 |
0.039 |
Tier III |
Other clones with same target sequence:
(none)
