Construct: sgRNA BRDN0001146212
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGGTCCATTTAGGTGAATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TXK (7294)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76360
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
48086555 |
- |
TXK |
NNNTCCATTTAGGTGAATGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
12496098 |
- |
SPIRE1 |
NNNTCCTTTTAGGTGAATGG |
NGA |
2 |
0.0304 |
Tier I |
| 3 |
NC_000014.9 |
14 |
61064986 |
+ |
SLC38A6 |
NNNTCCATTAAGATGAATGG |
NGG |
2 |
0.7912 |
Tier II |
| 4 |
NC_000008.11 |
8 |
55473058 |
- |
XKR4 |
NNNTCCAATTGGGTGAATGG |
NGG |
2 |
0.52 |
Tier II |
| 5 |
NC_000019.10 |
19 |
51969319 |
- |
ZNF350 |
NNNTCCAATTGGGTGAATGG |
NGG |
2 |
0.52 |
Tier II |
| 6 |
NC_000012.12 |
12 |
49423746 |
- |
SPATS2 |
NNNTCCATTTAGGTGAATTT |
NGG |
2 |
0.4667 |
Tier II |
| 7 |
NC_000008.11 |
8 |
11198641 |
+ |
XKR6 |
NNNAACATTTAGGTGAATGG |
NGG |
2 |
0.2721 |
Tier II |
| 8 |
NC_000001.11 |
1 |
171963867 |
- |
DNM3 |
NNNTCCATTCAGGTGGATGG |
NGG |
2 |
0.1026 |
Tier II |
| 9 |
NC_000015.10 |
15 |
79069958 |
- |
RASGRF1 |
NNNTCCATTTGGGTGAATGG |
NGC |
2 |
0.0144 |
Tier II |
| 10 |
NC_000008.11 |
8 |
84636916 |
- |
RALYL |
NNNTCCATTTAGGTGAAGGG |
NTG |
2 |
0.013 |
Tier II |
| 11 |
NC_000013.11 |
13 |
101223996 |
- |
NALCN |
NNNTCCATTTAGGTGTAAGG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000019.10 |
19 |
51969319 |
- |
ZNF350-AS1 |
NNNTCCAATTGGGTGAATGG |
NGG |
2 |
0.52 |
Tier III |
| 13 |
NC_000005.10 |
5 |
118240801 |
- |
LINC02147 |
NNNTCCATTTTGGTAAATGG |
NGG |
2 |
0.2896 |
Tier III |
| 14 |
NC_000013.11 |
13 |
106326321 |
+ |
LOC107984626 |
NNNTCCATTTTGGTGAATGA |
NGG |
2 |
0.2885 |
Tier III |
| 15 |
NC_000005.10 |
5 |
21617966 |
- |
LOC105374685 |
NNNTCCATTTAGGTGATTGT |
NGG |
2 |
0.0933 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
73356071 |
+ |
Slc38a6 |
NNNTCCATTAAGATGAATGG |
NGG |
2 |
0.7912 |
Tier II |
| 2 |
NC_000074.6 |
8 |
13438425 |
+ |
Tmem255b |
NNNTGCACTTAGGTGAATGG |
NGG |
2 |
0.6 |
Tier II |
| 3 |
NC_000077.6 |
11 |
80305216 |
+ |
Rhbdl3 |
NNNGCCATTTGGGTGAATGG |
NGG |
2 |
0.4063 |
Tier II |
| 4 |
NC_000081.6 |
15 |
73054807 |
+ |
Trappc9 |
NNNTCCAGTCAGGTGAATGG |
NGG |
2 |
0.3911 |
Tier II |
| 5 |
NC_000070.6 |
4 |
36945347 |
- |
Lingo2 |
NNNTCCATTTAGGTGAATAG |
NAG |
2 |
0.1852 |
Tier II |
| 6 |
NC_000075.6 |
9 |
107599309 |
+ |
Sema3b |
NNNTCCATCTAGGTGAGTGG |
NGG |
2 |
0.1629 |
Tier II |
| 7 |
NC_000067.6 |
1 |
38582495 |
- |
Aff3 |
NNNCCCATTTAGGTGACTGG |
NGG |
2 |
0.1142 |
Tier II |
| 8 |
NC_000081.6 |
15 |
41511404 |
+ |
Oxr1 |
NNNTCCATTTAGGTTAATGT |
NGG |
2 |
0.1 |
Tier II |
| 9 |
NC_000082.6 |
16 |
21884930 |
+ |
Map3k13 |
NNNTCCATTTAGGATAATGG |
NGG |
2 |
0.0884 |
Tier II |
| 10 |
NC_000086.7 |
X |
143762073 |
+ |
Pak3 |
NNNTCCATTTAGGTGAGGGG |
NGG |
2 |
0.0588 |
Tier II |
| 11 |
NC_000081.6 |
15 |
40723659 |
- |
Zfpm2 |
NNNTCCATTTAGGTGAATGG |
NTG |
1 |
0.039 |
Tier II |
| 12 |
NC_000069.6 |
3 |
13849866 |
- |
Ralyl |
NNNTCCATTTTGGTGAATGG |
NGA |
2 |
0.0214 |
Tier II |
| 13 |
NC_000079.6 |
13 |
61700887 |
- |
LOC115488202 |
NNNTCCTTTTATGTGAATGG |
NGG |
2 |
0.1683 |
Tier III |
| 14 |
NC_000079.6 |
13 |
101266689 |
+ |
4933416M06Rik |
NNNTCCCTTTAGGTGAAGGG |
NGG |
2 |
0.1458 |
Tier III |
| 15 |
NC_000079.6 |
13 |
101266689 |
+ |
Gm36427 |
NNNTCCCTTTAGGTGAAGGG |
NGG |
2 |
0.1458 |
Tier III |
| 16 |
NC_000070.6 |
4 |
8413888 |
- |
Gm42252 |
NNNTCCATTTAGGTTAATGT |
NGG |
2 |
0.1 |
Tier III |
Other clones with same target sequence:
(none)
