Construct: sgRNA BRDN0001146213
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AATACTTACTAGATCTAGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- STK24 (8428)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77057
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000013.11 |
13 |
98482273 |
+ |
STK24 |
NNNACTTACTAGATCTAGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
32092211 |
+ |
TNXB |
NNNACTCAGTAGATCTAGTG |
NGG |
2 |
0.4256 |
Tier II |
| 3 |
NC_000013.11 |
13 |
93940364 |
+ |
GPC6 |
NNNAGGTACTAGATCTAGTG |
NGG |
2 |
0.3429 |
Tier II |
| 4 |
NC_000023.11 |
X |
106181577 |
- |
PWWP3B |
NNNACTTCCTAGGTCTAGTG |
NGG |
2 |
0.2795 |
Tier II |
| 5 |
NC_000004.12 |
4 |
61818178 |
+ |
ADGRL3 |
NNNACTTACTAGATATAATG |
NGG |
2 |
0.1538 |
Tier II |
| 6 |
NC_000002.12 |
2 |
158287458 |
+ |
CCDC148 |
NNNACTTACTAGATACAGTG |
NGG |
2 |
0.1481 |
Tier II |
| 7 |
NC_000005.10 |
5 |
145993030 |
+ |
SH3RF2 |
NNNACTTACTAGATACAGTG |
NGG |
2 |
0.1481 |
Tier II |
| 8 |
NC_000004.12 |
4 |
168242646 |
- |
DDX60 |
NNNACTTCCTAGATATAGTG |
NGG |
2 |
0.0952 |
Tier II |
| 9 |
NC_000011.10 |
11 |
134080036 |
+ |
JAM3 |
NNNACTTACTAGATCTAGTG |
NTT |
2 |
0.0 |
Tier II |
| 10 |
NC_000008.11 |
8 |
75227051 |
+ |
CASC9 |
NNNTCTTACAAGATCTAGTG |
NGG |
2 |
0.5455 |
Tier III |
| 11 |
NC_000003.12 |
3 |
66928704 |
+ |
LOC105377144 |
NNNAATTATTAGATCTAGTG |
NGG |
2 |
0.5 |
Tier III |
| 12 |
NC_000005.10 |
5 |
162653631 |
+ |
LOC105377698 |
NNNACTTAGTAGATCTATTG |
NGG |
2 |
0.4127 |
Tier III |
| 13 |
NC_000005.10 |
5 |
145993030 |
+ |
LOC107986458 |
NNNACTTACTAGATACAGTG |
NGG |
2 |
0.1481 |
Tier III |
| 14 |
NC_000012.12 |
12 |
30272251 |
+ |
LINC02386 |
NNNACTTCCTAGATATAGTG |
NGG |
2 |
0.0952 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
43305858 |
- |
Atp8b5 |
NNNAATTACTAGGTCTAGTG |
NGG |
2 |
0.3727 |
Tier I |
| 2 |
NC_000081.6 |
15 |
74528871 |
- |
Adgrb1 |
NNNACTTAGTAGGTCTAGTG |
NGG |
2 |
0.4037 |
Tier II |
| 3 |
NC_000072.6 |
6 |
63420663 |
+ |
Grid2 |
NNNACTTACTACATCTATTG |
NGG |
2 |
0.3529 |
Tier II |
| 4 |
NC_000068.7 |
2 |
125986664 |
+ |
Fam227b |
NNNACTTACCAGATCTTGTG |
NGG |
2 |
0.0711 |
Tier II |
| 5 |
NC_000077.6 |
11 |
105107188 |
+ |
Efcab3 |
NNNCCTTACTAGATTTAGTG |
NGG |
2 |
0.0235 |
Tier II |
| 6 |
NC_000075.6 |
9 |
55742483 |
- |
Scaper |
NNNACTTACTAGTTTTAGTG |
NGG |
2 |
0.02 |
Tier II |
| 7 |
NC_000072.6 |
6 |
94209856 |
- |
Magi1 |
NNNACTTACTAGATGGAGTG |
NGG |
2 |
0.0173 |
Tier II |
| 8 |
NC_000078.6 |
12 |
57127076 |
- |
Slc25a21 |
NNNACTTACTAGATTTGGTG |
NGG |
2 |
0.0118 |
Tier II |
| 9 |
NC_000072.6 |
6 |
94209856 |
- |
4930511A08Rik |
NNNACTTACTAGATGGAGTG |
NGG |
2 |
0.0173 |
Tier III |
| 10 |
NC_000072.6 |
6 |
94209856 |
- |
Gm52867 |
NNNACTTACTAGATGGAGTG |
NGG |
2 |
0.0173 |
Tier III |
Other clones with same target sequence:
(none)
