Construct: sgRNA BRDN0001146214
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGAGGTTCGTCTAGTAAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NADK2 (133686)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78091
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
36226536 |
- |
NADK2 |
NNNAGGTTCGTCTAGTAAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
42132064 |
+ |
BEND4 |
NNNAGGTGCGTCTAGAAAAG |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000007.14 |
7 |
14487368 |
+ |
DGKB |
NNNAGGTTAGACTAGTAAAG |
NGG |
2 |
0.6429 |
Tier II |
| 4 |
NC_000007.14 |
7 |
93348586 |
+ |
VPS50 |
NNNAGGTTCGTCCACTAAAG |
NGG |
2 |
0.2153 |
Tier II |
| 5 |
NC_000001.11 |
1 |
3391462 |
- |
PRDM16 |
NNNAGCTTCGTCTAGTAAAG |
NGA |
2 |
0.0473 |
Tier II |
| 6 |
NC_000014.9 |
14 |
20602100 |
+ |
RNASE11-AS1 |
NNNAGGTTCGTGTAGTAAAG |
NCG |
2 |
0.0476 |
Tier III |
| 7 |
NC_000001.11 |
1 |
208310371 |
+ |
LOC105372889 |
NNNAGGTTCGTCTAGTGAAG |
NGT |
2 |
0.0028 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
20989823 |
+ |
Vcl |
NNNAGGTTCCTCTAGAAAAG |
NGG |
2 |
0.3636 |
Tier II |
| 2 |
NC_000080.6 |
14 |
65609675 |
+ |
Nuggc |
NNNAGGTTCGTGTAGTAAAC |
NGG |
2 |
0.1905 |
Tier II |
| 3 |
NC_000078.6 |
12 |
53344839 |
- |
Npas3 |
NNNAGGTTCATCTAGTCAAG |
NGG |
2 |
0.1647 |
Tier II |
Other clones with same target sequence:
(none)
