Construct: sgRNA BRDN0001146215
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTACCTCTTAAATCAACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRPF4B (8899)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76431
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
4032404 |
- |
PRP4K |
NNNACCTCTTAAATCAACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
147858479 |
- |
SAMD5 |
NNNACCTCATAAATCAACTA |
NGG |
2 |
0.8705 |
Tier II |
| 3 |
NC_000007.14 |
7 |
147679784 |
- |
CNTNAP2 |
NNNACTTCTTAAATCAACTA |
NGG |
2 |
0.8705 |
Tier II |
| 4 |
NC_000010.11 |
10 |
74295812 |
+ |
ADK |
NNNACTTCTTAAATCAACTT |
NGG |
2 |
0.65 |
Tier II |
| 5 |
NC_000015.10 |
15 |
50058192 |
+ |
ATP8B4 |
NNNTCCTTTTAAATCAACTG |
NGG |
2 |
0.5568 |
Tier II |
| 6 |
NC_000005.10 |
5 |
94193057 |
- |
KIAA0825 |
NNNACCTCTCAAATCAACTA |
NGG |
2 |
0.5 |
Tier II |
| 7 |
NC_000005.10 |
5 |
36911018 |
- |
NIPBL |
NNNTCCTCTTAATTCAACTG |
NGG |
2 |
0.1909 |
Tier II |
| 8 |
NC_000001.11 |
1 |
186456838 |
+ |
PDC |
NNNGCCTCTTAAATCAACTG |
NAG |
2 |
0.162 |
Tier II |
| 9 |
NC_000023.11 |
X |
72034449 |
+ |
NHSL2 |
NNNACCTCATAAATTAACTG |
NGG |
2 |
0.0619 |
Tier II |
| 10 |
NC_000016.10 |
16 |
17164818 |
+ |
XYLT1 |
NNNACTTCTTAAATCAACTG |
NGC |
2 |
0.0206 |
Tier II |
| 11 |
NC_000002.12 |
2 |
171125016 |
+ |
TLK1 |
NNNAACTCTTAAATCAACTG |
NGC |
2 |
0.0127 |
Tier II |
| 12 |
NC_000002.12 |
2 |
171125016 |
+ |
LOC124906092 |
NNNAACTCTTAAATCAACTG |
NGC |
2 |
0.0127 |
Tier II |
| 13 |
NC_000001.11 |
1 |
171709063 |
- |
VAMP4 |
NNNACTTCTTAAATCCACTG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000005.10 |
5 |
94193057 |
- |
LOC105379087 |
NNNACCTCTCAAATCAACTA |
NGG |
2 |
0.5 |
Tier III |
| 15 |
NC_000009.12 |
9 |
114846468 |
- |
LOC645266 |
NNNACCTCTTAAAACAACAG |
NGG |
2 |
0.1769 |
Tier III |
| 16 |
NC_000001.11 |
1 |
186456838 |
+ |
PDC-AS1 |
NNNGCCTCTTAAATCAACTG |
NAG |
2 |
0.162 |
Tier III |
| 17 |
NC_000011.10 |
11 |
130705730 |
+ |
LINC02873 |
NNNACCTCTTAGATCACCTG |
NGG |
2 |
0.1275 |
Tier III |
| 18 |
NC_000004.12 |
4 |
177980396 |
- |
LINC01098 |
NNNACCTCTTAACTAAACTG |
NGG |
2 |
0.0468 |
Tier III |
| 19 |
NC_000023.11 |
X |
128625014 |
- |
LOC107985698 |
NNNACCTCTTAAATTGACTG |
NGG |
2 |
0.0128 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000079.6 |
13 |
34884076 |
- |
Prpf4b |
NNNATCTCTTAAGTCAACTG |
NGG |
2 |
0.415 |
Tier I |
| 2 |
NC_000069.6 |
3 |
54311347 |
- |
Trpc4 |
NNNACATCTTGAATCAACTG |
NGG |
2 |
0.6036 |
Tier II |
| 3 |
NC_000072.6 |
6 |
77513899 |
- |
Ctnna2 |
NNNACCTCTTGAAACAACTG |
NGG |
2 |
0.4024 |
Tier II |
| 4 |
NC_000075.6 |
9 |
58590209 |
- |
Nptn |
NNNAACTGTTAAATCAACTG |
NGG |
2 |
0.3673 |
Tier II |
| 5 |
NC_000069.6 |
3 |
79532784 |
- |
Fnip2 |
NNNATGTCTTAAATCAACTG |
NGG |
2 |
0.3182 |
Tier II |
| 6 |
NC_000068.7 |
2 |
79596815 |
+ |
Itprid2 |
NNNACCCCTTAAATCAACTG |
NAG |
2 |
0.1782 |
Tier II |
| 7 |
NC_000078.6 |
12 |
78896934 |
+ |
Plek2 |
NNNAGCTCTTAAACCAACTG |
NGG |
2 |
0.1714 |
Tier II |
| 8 |
NC_000081.6 |
15 |
99643202 |
- |
Racgap1 |
NNNATCTCTTAAATCAACGG |
NGG |
2 |
0.1591 |
Tier II |
| 9 |
NC_000067.6 |
1 |
134468525 |
- |
Klhl12 |
NNNACCTCTTATATAAACTG |
NGG |
2 |
0.0741 |
Tier II |
| 10 |
NC_000067.6 |
1 |
110140895 |
- |
Cdh7 |
NNNACCTCTTAAGTCAACTG |
NTG |
2 |
0.0254 |
Tier II |
| 11 |
NC_000081.6 |
15 |
96424298 |
- |
Scaf11 |
NNNACCTATTAAATCAACTG |
NGT |
2 |
0.0105 |
Tier II |
| 12 |
NC_000078.6 |
12 |
16693699 |
+ |
Greb1 |
NNNACCTCTTAAATTATCTG |
NGG |
2 |
0.0089 |
Tier II |
| 13 |
NC_000072.6 |
6 |
17880763 |
+ |
St7 |
NNNACCTCTTAAATCTAATG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000084.6 |
18 |
66510113 |
- |
Gm9926 |
NNNACCTTTTAAAACAACTG |
NGG |
2 |
0.5417 |
Tier III |
| 15 |
NC_000073.6 |
7 |
79921999 |
- |
5430400D12Rik |
NNNACCTCTTTAATCCACTG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
