Construct: sgRNA BRDN0001146216
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCAGCGCCAGTACTTCAAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPS6KA4 (8986)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77920
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
64360531 |
+ |
RPS6KA4 |
NNNGCGCCAGTACTTCAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
21989014 |
- |
XPO7 |
NNNGCCCCAGTACTTCAAGT |
NGG |
2 |
0.4773 |
Tier I |
| 3 |
NC_000015.10 |
15 |
75368565 |
+ |
MAN2C1 |
NNNGCGCCAGTGCTTCAAGG |
NCG |
2 |
0.0774 |
Tier I |
| 4 |
NC_000019.10 |
19 |
16360509 |
+ |
EPS15L1 |
NNNACCCCAGTACTTCAAGG |
NGG |
2 |
0.6136 |
Tier II |
| 5 |
NC_000001.11 |
1 |
197476232 |
- |
CRB1 |
NNNGCTCAAGTACTTCAAGG |
NGG |
2 |
0.4333 |
Tier II |
| 6 |
NC_000005.10 |
5 |
139915074 |
+ |
NRG2 |
NNNGCTCCAGTAATTCAAGG |
NGG |
2 |
0.2564 |
Tier II |
| 7 |
NC_000014.9 |
14 |
104788705 |
+ |
AKT1 |
NNNGTGCCAGGACTTCAAGG |
NGG |
2 |
0.2545 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
6839314 |
- |
Rps6ka4 |
NNNGCGCCAGTACTTCAAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000070.6 |
4 |
117760433 |
- |
Klf17 |
NNNGCCCCAGTACTTCAAGA |
NGG |
2 |
0.6392 |
Tier I |
| 3 |
NC_000078.6 |
12 |
73598353 |
+ |
Prkch |
NNNGCACCAGTGCTTCAAGG |
NGG |
2 |
0.7222 |
Tier II |
| 4 |
NC_000072.6 |
6 |
85809518 |
- |
Nat8f6 |
NNNCCGCCAGTACTTCAAGG |
NGA |
2 |
0.0368 |
Tier II |
| 5 |
NC_000079.6 |
13 |
37873013 |
- |
Rreb1 |
NNNGCCCCAGTACTTCAAGG |
NTG |
2 |
0.0266 |
Tier II |
| 6 |
NC_000079.6 |
13 |
10021635 |
- |
Chrm3 |
NNNGCGCCAGTACTTCAGGG |
NGC |
2 |
0.0089 |
Tier II |
Other clones with same target sequence:
(none)
