Construct: sgRNA BRDN0001146218
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTAAGGGCAAGATCGAAACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HKDC1 (80201)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76828
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
69246209 |
+ |
HKDC1 |
NNNAGGGCAAGATCGAAACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
3087076 |
+ |
TSPAN9 |
NNNAGAGCAAGATTGAAACA |
NGG |
2 |
0.7333 |
Tier II |
| 3 |
NC_000005.10 |
5 |
171176926 |
+ |
RANBP17 |
NNNAGGGCAAGAATGAAACA |
NGG |
2 |
0.5077 |
Tier II |
| 4 |
NC_000009.12 |
9 |
15716007 |
+ |
CCDC171 |
NNNAGGGAGAGATCGAAACA |
NGG |
2 |
0.4333 |
Tier II |
| 5 |
NC_000004.12 |
4 |
23969823 |
+ |
PPARGC1A |
NNNAGGGCAGCATCGAAACA |
NGG |
2 |
0.2381 |
Tier II |
| 6 |
NC_000010.11 |
10 |
25963806 |
+ |
MYO3A |
NNNAGGGCAGGATAGAAACA |
NGG |
2 |
0.1944 |
Tier II |
| 7 |
NC_000005.10 |
5 |
61436984 |
- |
ZSWIM6 |
NNNAGGGCAAGATCGAAGAA |
NGG |
2 |
0.1714 |
Tier II |
| 8 |
NC_000001.11 |
1 |
39104829 |
- |
MACF1 |
NNNAGGGCAAGATCCAGACA |
NGG |
2 |
0.0481 |
Tier II |
| 9 |
NC_000003.12 |
3 |
36436291 |
- |
STAC |
NNNAAGGCAAGATGGAAACA |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000009.12 |
9 |
101388371 |
- |
MRPL50 |
NNNAGGGCAAGATGGAAATA |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000015.10 |
15 |
100495286 |
+ |
CERS3 |
NNNAGGGCAAGATGGGAACA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000004.12 |
4 |
124277736 |
+ |
LOC105377406 |
NNNAGGGCAAGATTCAAACA |
NGG |
2 |
0.2 |
Tier III |
| 13 |
NC_000013.11 |
13 |
112036415 |
+ |
SOX1-OT |
NNNAGGGCAAGATCGACACA |
NAG |
2 |
0.0458 |
Tier III |
| 14 |
NC_000003.12 |
3 |
36436291 |
- |
LOC124906227 |
NNNAAGGCAAGATGGAAACA |
NGG |
2 |
0.0 |
Tier III |
| 15 |
NC_000007.14 |
7 |
94386481 |
- |
COL1A2-AS1 |
NNNAGGGCAAGATGGAAACC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
62403518 |
- |
Hkdc1 |
NNNAGGGCAAGATCGAAACA |
NAG |
1 |
0.2593 |
Tier I |
| 2 |
NC_000079.6 |
13 |
69813798 |
+ |
Med10 |
NNNAGGGCAAGATCGACACA |
NTG |
2 |
0.0069 |
Tier I |
| 3 |
NC_000078.6 |
12 |
88459553 |
- |
Adck1 |
NNNAGGGCCAGATAGAAACA |
NGG |
2 |
0.2 |
Tier II |
| 4 |
NC_000073.6 |
7 |
90782570 |
- |
Dlg2 |
NNNAGGGCAAGATTGAAAGA |
NGG |
2 |
0.0917 |
Tier II |
| 5 |
NC_000070.6 |
4 |
49293980 |
+ |
Plppr1 |
NNNTGGGCAAGATCTAAACA |
NGG |
2 |
0.0909 |
Tier II |
| 6 |
NC_000085.6 |
19 |
3497986 |
+ |
Ppp6r3 |
NNNAGGGCAAGATGAAAACA |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000073.6 |
7 |
140874481 |
- |
Sirt3 |
NNNAGGGCAAGATGGAAACA |
NTG |
2 |
0.0 |
Tier II |
| 8 |
NC_000067.6 |
1 |
144160771 |
- |
Rgs13 |
NNNAGGGCATGATGGAAACA |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000067.6 |
1 |
156217345 |
- |
Gm30472 |
NNNAAGGCAAGATTGAAACA |
NGG |
2 |
0.6356 |
Tier III |
| 10 |
NC_000077.6 |
11 |
121789888 |
- |
Gm12589 |
NNNAAGGCAAGATCCAAACA |
NGG |
2 |
0.2364 |
Tier III |
Other clones with same target sequence:
(none)
