Construct: sgRNA BRDN0001146219
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTCCGAGGCTCATCACCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CHKA (1119)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76587
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000011.10 |
11 |
68097072 |
+ |
CHKA |
NNNCCGAGGCTCATCACCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
53119726 |
- |
SLC1A7 |
NNNCTGAGGCTCATTACCAA |
NGG |
2 |
0.0424 |
Tier II |
| 3 |
NC_000017.11 |
17 |
20976894 |
+ |
LOC124900389 |
NNNCCCAGGCTCATCCCCAA |
NGG |
2 |
0.0 |
Tier II |
| 4 |
NC_000017.11 |
17 |
20976894 |
+ |
LOC339260 |
NNNCCCAGGCTCATCCCCAA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
132499261 |
- |
Sesn2 |
NNNCCGTGGCTCATCACCAA |
NGA |
2 |
0.0304 |
Tier I |
| 2 |
NC_000085.6 |
19 |
3864707 |
- |
Chka |
NNNCCGAGGCTCATCGCCAA |
NGC |
2 |
0.0043 |
Tier I |
| 3 |
NC_000083.6 |
17 |
28897610 |
+ |
Bnip5 |
NNNTCGAGGTTCATCACCAA |
NGG |
2 |
0.7529 |
Tier II |
| 4 |
NC_000071.6 |
5 |
21779755 |
+ |
Dnajc2 |
NNNCCAAGGCTCATCACCAT |
NGG |
2 |
0.6 |
Tier II |
| 5 |
NC_000068.7 |
2 |
14250751 |
- |
Mrc1 |
NNNCTGAGGCACATCACCAA |
NGG |
2 |
0.4773 |
Tier II |
| 6 |
NC_000067.6 |
1 |
191338700 |
- |
Pacc1 |
NNNCCGACCCTCATCACCAA |
NGG |
2 |
0.3314 |
Tier II |
| 7 |
NC_000070.6 |
4 |
59771204 |
- |
Inip |
NNNCCGAGCCTCATCACCAT |
NGG |
2 |
0.3231 |
Tier II |
| 8 |
NC_000075.6 |
9 |
47842484 |
- |
Cadm1 |
NNNCCGAGTCTCATCACCTA |
NGG |
2 |
0.2872 |
Tier II |
| 9 |
NC_000078.6 |
12 |
70205226 |
+ |
Pygl |
NNNCCGTGGCTTATCACCAA |
NGG |
2 |
0.2356 |
Tier II |
| 10 |
NC_000086.7 |
X |
56128575 |
- |
3830403N18Rik |
NNNCCAAGGCTCATCACCCA |
NGG |
2 |
0.2069 |
Tier II |
| 11 |
NC_000085.6 |
19 |
34968549 |
- |
Kif20b |
NNNCTGAGGCTCATCACCAC |
NGG |
2 |
0.1446 |
Tier II |
| 12 |
NC_000067.6 |
1 |
153247520 |
+ |
Lamc1 |
NNNCAGAGGCTCATCACCAC |
NGG |
2 |
0.1299 |
Tier II |
| 13 |
NC_000073.6 |
7 |
41643723 |
- |
Zfp788 |
NNNCCGTGGCTCATGACCAA |
NGG |
2 |
0.0219 |
Tier II |
| 14 |
NC_000078.6 |
12 |
103751410 |
+ |
Gm17042 |
NNNCCCAGGCTCATCACCCA |
NGG |
2 |
0.1411 |
Tier III |
Other clones with same target sequence:
(none)
