Construct: sgRNA BRDN0001146223
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGCTTCACTAGCAGTCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMP2K (55589)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75928
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
78861425 |
- |
BMP2K |
NNNGCTTCACTAGCAGTCAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000002.12 |
2 |
79185856 |
- |
CTNNA2 |
NNNCCTTCACTAACAGTCAT |
NGG |
2 |
0.4887 |
Tier II |
| 3 |
NC_000012.12 |
12 |
110483387 |
- |
FAM216A |
NNNGCTTCACTAGCAGTTTT |
NGG |
2 |
0.3462 |
Tier II |
| 4 |
NC_000007.14 |
7 |
96009259 |
- |
DYNC1I1 |
NNNGCTTCACGGGCAGTCAT |
NGG |
2 |
0.2889 |
Tier II |
| 5 |
NC_000010.11 |
10 |
21971229 |
- |
DNAJC1 |
NNNTATTCACTAGCAGTCAT |
NGG |
2 |
0.2078 |
Tier II |
| 6 |
NC_000022.11 |
22 |
19096006 |
- |
DGCR2 |
NNNGCTTCAGTACCAGTCAT |
NGG |
2 |
0.1637 |
Tier II |
| 7 |
NC_000005.10 |
5 |
83267444 |
+ |
XRCC4 |
NNNGCCTCACTAGCAGTGAT |
NGG |
2 |
0.1212 |
Tier II |
| 8 |
NC_000020.11 |
20 |
63252706 |
- |
NKAIN4 |
NNNGCTTCACTAGCAGACCT |
NGG |
2 |
0.1103 |
Tier II |
| 9 |
NC_000006.12 |
6 |
57066453 |
+ |
KIAA1586 |
NNNGCTTCACTGGGAGTCAT |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000008.11 |
8 |
128091851 |
+ |
PVT1 |
NNNGCATCATTAGCAGTCAT |
NGG |
2 |
0.8157 |
Tier III |
| 11 |
NC_000014.9 |
14 |
54175595 |
- |
LOC105370507 |
NNNGCTTTTCTAGCAGTCAT |
NGG |
2 |
0.525 |
Tier III |
| 12 |
NC_000012.12 |
12 |
110483387 |
- |
LOC124903016 |
NNNGCTTCACTAGCAGTTTT |
NGG |
2 |
0.3462 |
Tier III |
| 13 |
NC_000012.12 |
12 |
54924697 |
- |
LOC105369778 |
NNNGCATCACTATCAGTCAT |
NGG |
2 |
0.26 |
Tier III |
| 14 |
NC_000022.11 |
22 |
19096006 |
- |
LOC124905078 |
NNNGCTTCAGTACCAGTCAT |
NGG |
2 |
0.1637 |
Tier III |
| 15 |
NC_000002.12 |
2 |
122251865 |
+ |
LOC105373592 |
NNNGCTTCACTAAGAGTCAT |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
97054888 |
- |
Bmp2k |
NNNGCTTCAGTAGCAGTCAT |
NGG |
1 |
0.3889 |
Tier I |
| 2 |
NC_000076.6 |
10 |
103073687 |
+ |
Lrriq1 |
NNNGCTTCCCTAGCAGTCAT |
NGG |
1 |
0.5714 |
Tier II |
| 3 |
NC_000074.6 |
8 |
38057976 |
- |
Sgcz |
NNNGCTTCACCAGCAGCCAT |
NGG |
2 |
0.4706 |
Tier II |
| 4 |
NC_000073.6 |
7 |
98195803 |
+ |
B3gnt6 |
NNNGCTTCACTGGCAGTCAA |
NGG |
2 |
0.4062 |
Tier II |
| 5 |
NC_000071.6 |
5 |
123496687 |
- |
Lrrc43 |
NNNGCTTGTCTAGCAGTCAT |
NGG |
2 |
0.3857 |
Tier II |
| 6 |
NC_000072.6 |
6 |
82919912 |
+ |
Sema4f |
NNNGCTTCTCTAGCAGACAT |
NGG |
2 |
0.32 |
Tier II |
| 7 |
NC_000073.6 |
7 |
39575016 |
- |
Gm33989 |
NNNGATTCACTAGCAGTGAT |
NGG |
2 |
0.0762 |
Tier II |
| 8 |
NC_000071.6 |
5 |
69563839 |
+ |
Guf1 |
NNNGCTTCACAAGCAGTCAT |
NTG |
2 |
0.0292 |
Tier II |
| 9 |
NC_000067.6 |
1 |
70639477 |
+ |
Spag16 |
NNNGCTTCACTAGCAGTCAG |
NGA |
2 |
0.0123 |
Tier II |
| 10 |
NC_000068.7 |
2 |
144910279 |
- |
Gm39952 |
NNNGCTTCTCAAGCAGTCAT |
NGG |
2 |
0.45 |
Tier III |
| 11 |
NC_000082.6 |
16 |
24380507 |
+ |
Gm4521 |
NNNGCTTCAGTAGCAGTCAT |
NGG |
1 |
0.3889 |
Tier III |
Other clones with same target sequence:
(none)
