Construct: sgRNA BRDN0001146229
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGCACACCTTTGACTATAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMPR2 (659)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76389
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
202555401 |
+ |
BMPR2 |
NNNCACACCTTTGACTATAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
50635908 |
- |
MAPK4 |
NNNCACACCATGGACTATAG |
NGG |
2 |
0.4286 |
Tier II |
| 3 |
NC_000009.12 |
9 |
76885064 |
+ |
PRUNE2 |
NNNCACACCTTTCACCATAG |
NGG |
2 |
0.2807 |
Tier II |
| 4 |
NC_000001.11 |
1 |
11994016 |
- |
MFN2 |
NNNCACACCTTTGACTACAG |
NTG |
2 |
0.0167 |
Tier II |
| 5 |
NC_000015.10 |
15 |
45057003 |
+ |
SORD |
NNNCACACCTTTGACTACAG |
NTG |
2 |
0.0167 |
Tier II |
| 6 |
NC_000008.11 |
8 |
130129750 |
+ |
ASAP1 |
NNNCACACCTTTGACTCTAG |
NTG |
2 |
0.0069 |
Tier II |
| 7 |
NC_000008.11 |
8 |
126762338 |
- |
LOC105375751 |
NNNGACCCCTTTGACTATAG |
NGG |
2 |
0.2188 |
Tier III |
| 8 |
NC_000016.10 |
16 |
80213548 |
+ |
DYNLRB2-AS1 |
NNNCACACCTTTGCCTATGG |
NGG |
2 |
0.0804 |
Tier III |
| 9 |
NC_000020.11 |
20 |
11356740 |
+ |
LOC105372529 |
NNNCACACCTTTGACTATAG |
NGA |
1 |
0.0694 |
Tier III |
| 10 |
NC_000021.9 |
21 |
9787996 |
+ |
LINC01667 |
NNNCACACCTTTGATTATAG |
NGG |
1 |
0.0667 |
Tier III |
| 11 |
NC_000007.14 |
7 |
24308750 |
+ |
LOC107986777 |
NNNCACACCTTAGACTATAG |
NTG |
2 |
0.0312 |
Tier III |
| 12 |
NC_000002.12 |
2 |
126556902 |
- |
YWHAZP2 |
NNNCACACCTTTGACTACAG |
NTG |
2 |
0.0167 |
Tier III |
| 13 |
NC_000002.12 |
2 |
126556902 |
- |
LOC105373602 |
NNNCACACCTTTGACTACAG |
NTG |
2 |
0.0167 |
Tier III |
| 14 |
NC_000010.11 |
10 |
105688052 |
+ |
YWHAZP5 |
NNNCACACCTTTGACTACAG |
NTG |
2 |
0.0167 |
Tier III |
| 15 |
NC_000010.11 |
10 |
105688052 |
+ |
LINC02627 |
NNNCACACCTTTGACTACAG |
NTG |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
151245332 |
- |
Camta1 |
NNNCACACATGTGACTATAG |
NGG |
2 |
0.3429 |
Tier II |
| 2 |
NC_000069.6 |
3 |
100836617 |
- |
Vtcn1 |
NNNCACACCTTTCACTAAAG |
NGG |
2 |
0.2807 |
Tier II |
| 3 |
NC_000079.6 |
13 |
103874471 |
- |
Erbin |
NNNCACATCTTTTACTATAG |
NGG |
2 |
0.2625 |
Tier II |
| 4 |
NC_000067.6 |
1 |
134695325 |
- |
Syt2 |
NNNCACACCTGGGACTATAG |
NGG |
2 |
0.2 |
Tier II |
| 5 |
NC_000078.6 |
12 |
41724020 |
- |
Immp2l |
NNNCACATCTTTGCCTATAG |
NGG |
2 |
0.1875 |
Tier II |
| 6 |
NC_000071.6 |
5 |
144251158 |
- |
Bri3 |
NNNCACACCTTTTACTGTAG |
NGG |
2 |
0.0529 |
Tier II |
| 7 |
NC_000078.6 |
12 |
44889518 |
- |
Stxbp6 |
NNNCACACCTTTGAGTATAT |
NGG |
2 |
0.035 |
Tier II |
| 8 |
NC_000077.6 |
11 |
66943088 |
- |
Gm12298 |
NNNCACACCTTTGGCTATTG |
NGG |
2 |
0.2513 |
Tier III |
| 9 |
NC_000067.6 |
1 |
140627968 |
- |
4930590L20Rik |
NNNCCCACCTTTCACTATAG |
NGG |
2 |
0.2105 |
Tier III |
| 10 |
NC_000068.7 |
2 |
21105253 |
- |
Gm13377 |
NNNCACACCTTTGACTACAG |
NAG |
2 |
0.1111 |
Tier III |
| 11 |
NC_000071.6 |
5 |
144251158 |
- |
2900089D17Rik |
NNNCACACCTTTTACTGTAG |
NGG |
2 |
0.0529 |
Tier III |
| 12 |
NC_000079.6 |
13 |
113667403 |
- |
Gm34586 |
NNNCACACCTATGACTATAG |
NGA |
2 |
0.0521 |
Tier III |
Other clones with same target sequence:
(none)
