Construct: sgRNA BRDN0001146230
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGATGGTACGCGGCTGCACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PFKFB3 (5209)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76462
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
6220766 |
- |
PFKFB3 |
NNNTGGTACGCGGCTGCACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
62395269 |
+ |
CABLES2 |
NNNTGGGACGCCGCTGCACG |
NGG |
2 |
0.3114 |
Tier II |
| 3 |
NC_000004.12 |
4 |
154724584 |
+ |
NDUFB2P1 |
NNNGGGGACGCGGCTGCACG |
NGG |
2 |
0.3676 |
Tier III |
| 4 |
NC_000004.12 |
4 |
154724584 |
+ |
LOC124900169 |
NNNGGGGACGCGGCTGCACG |
NGG |
2 |
0.3676 |
Tier III |
| 5 |
NC_000007.14 |
7 |
65750956 |
- |
LINC03006 |
NNNTGGTCCGCGGCTGCACG |
NTG |
2 |
0.0167 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
11484755 |
+ |
Pfkfb3 |
NNNTGGTACGGGGCTGCACG |
NGG |
1 |
0.25 |
Tier I |
| 2 |
NC_000083.6 |
17 |
14978771 |
- |
Gm3417 |
NNNCGGGACGCGGCTGCACG |
NGG |
2 |
0.3806 |
Tier II |
| 3 |
NC_000083.6 |
17 |
15010143 |
- |
Gm3435 |
NNNCGGGACGCGGCTGCACG |
NGG |
2 |
0.3806 |
Tier II |
| 4 |
NC_000083.6 |
17 |
15010143 |
- |
Gm3448 |
NNNCGGGACGCGGCTGCACG |
NGG |
2 |
0.3806 |
Tier II |
| 5 |
NC_000083.6 |
17 |
15041518 |
- |
Tcte3 |
NNNCGGGACGCGGCTGCACG |
NGG |
2 |
0.3806 |
Tier II |
Other clones with same target sequence:
(none)
