Construct: sgRNA BRDN0001146231
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTGGACACTAGAACCCCCAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NRK (203447)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76313
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
105909530 |
+ |
NRK |
NNNGACACTAGAACCCCCAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
19097106 |
+ |
SLC25A42 |
NNNGAAACCAGAACCCCCAC |
NGG |
2 |
0.8571 |
Tier II |
| 3 |
NC_000007.14 |
7 |
4096840 |
+ |
SDK1 |
NNNGACACTGAAACCCCCAC |
NGG |
2 |
0.5556 |
Tier II |
| 4 |
NC_000005.10 |
5 |
168886540 |
- |
SLIT3 |
NNNGACACTAAAACCCCAAC |
NGG |
2 |
0.5385 |
Tier II |
| 5 |
NC_000023.11 |
X |
17834300 |
+ |
RAI2 |
NNNGATACTAGAACACCCAC |
NGG |
2 |
0.2063 |
Tier II |
| 6 |
NC_000012.12 |
12 |
130560464 |
+ |
RIMBP2 |
NNNGCCACTAGACCCCCCAC |
NGG |
2 |
0.1053 |
Tier II |
| 7 |
NC_000019.10 |
19 |
18785628 |
- |
COMP |
NNNGACCCTAGAACCCCCAC |
NGT |
2 |
0.0071 |
Tier II |
| 8 |
NC_000022.11 |
22 |
43832958 |
+ |
SULT4A1 |
NNNGACACTAGCAGCCCCAC |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000006.12 |
6 |
9005832 |
- |
LOC105374914 |
NNNGACACTACAACCCCCAC |
NGT |
2 |
0.0069 |
Tier III |
| 10 |
NC_000013.11 |
13 |
64316877 |
- |
LOC124903237 |
NNNGACACTAGAAGTCCCAC |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
79694230 |
+ |
Ticrr |
NNNGACACAAGAACCTCCAC |
NGG |
2 |
0.2857 |
Tier I |
| 2 |
NC_000077.6 |
11 |
119283760 |
+ |
Gaa |
NNNGACACTGGAAGCCCCAC |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000077.6 |
11 |
60280365 |
+ |
Tom1l2 |
NNNGAGACTAGAACCACCAC |
NGG |
2 |
0.5 |
Tier II |
| 4 |
NC_000068.7 |
2 |
167974461 |
+ |
Ptpn1 |
NNNGACTCTAGAACACCCAC |
NGG |
2 |
0.0972 |
Tier II |
| 5 |
NC_000071.6 |
5 |
113536098 |
+ |
Wscd2 |
NNNGACACTAGAACCAGCAC |
NGG |
2 |
0.0588 |
Tier II |
| 6 |
NC_000079.6 |
13 |
103014831 |
+ |
Mast4 |
NNNGACACTAGATCCCCGAC |
NGG |
2 |
0.04 |
Tier II |
| 7 |
NC_000072.6 |
6 |
127508571 |
- |
Parp11 |
NNNGACACTAGAACCCTCAC |
NGA |
2 |
0.0324 |
Tier II |
| 8 |
NC_000071.6 |
5 |
113536098 |
+ |
Gm52822 |
NNNGACACTAGAACCAGCAC |
NGG |
2 |
0.0588 |
Tier III |
Other clones with same target sequence:
(none)
