Construct: sgRNA BRDN0001146233
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGCCGGACGGCGTGCAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NME2 (4831)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77933
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
51166881 |
+ |
NME2 |
NNNCCGGACGGCGTGCAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
51166881 |
+ |
NME1-NME2 |
NNNCCGGACGGCGTGCAGCG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000005.10 |
5 |
69189741 |
+ |
CENPH |
NNNCCAGGCGGCGTGCAGCG |
NGG |
2 |
0.7333 |
Tier I |
| 4 |
NC_000016.10 |
16 |
1771356 |
- |
NME3 |
NNNCCGGACGGCGTGCAGCG |
NCG |
1 |
0.1071 |
Tier I |
| 5 |
NC_000017.11 |
17 |
82719469 |
- |
FN3KRP |
NNNGCGGCCGGCGTGCAGCG |
NGG |
2 |
0.2143 |
Tier II |
| 6 |
NC_000001.11 |
1 |
243197186 |
+ |
CEP170 |
NNNCAGGACGGCGGGCAGCG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000004.12 |
4 |
51844395 |
+ |
DCUN1D4 |
NNNCCGGACGGCGGGAAGCG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000002.12 |
2 |
132279509 |
+ |
RNA28SP |
NNNCCGGCCGGCGTGCAGCT |
NGG |
2 |
0.3 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
93955571 |
- |
Nme2 |
NNNCCAGATGGCGTGCAGCG |
NGG |
2 |
0.875 |
Tier I |
| 2 |
NC_000083.6 |
17 |
24896683 |
+ |
Nme3 |
NNNCCCGACGGCGTGCAGCG |
NCG |
2 |
0.0731 |
Tier I |
| 3 |
NC_000070.6 |
4 |
126236986 |
+ |
Map7d1 |
NNNCCGGACGGGGTGCAGCG |
NTG |
2 |
0.0173 |
Tier I |
| 4 |
NC_000073.6 |
7 |
19076538 |
- |
Dmwd |
NNNGCGGACGGCGGGCAGCG |
NGG |
2 |
0.0 |
Tier I |
| 5 |
NC_000070.6 |
4 |
149737818 |
+ |
Tmem201 |
NNNCCGGGCCGCGTGCAGCG |
NGG |
2 |
0.2933 |
Tier II |
| 6 |
NC_000074.6 |
8 |
70010953 |
- |
Gm7730 |
NNNCCAGATGGCGTGCAGCG |
NGG |
2 |
0.875 |
Tier III |
Other clones with same target sequence:
(none)
