Construct: sgRNA BRDN0001146234
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGCAGAAGAAAGTTCGCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- KALRN (8997)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77637
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
124632625 |
+ |
KALRN |
NNNCAGAAGAAAGTTCGCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000016.10 |
16 |
19827079 |
+ |
IQCK |
NNNCAGAAGAAACTTCGCGA |
NGC |
2 |
0.0094 |
Tier I |
| 3 |
NC_000020.11 |
20 |
19318994 |
+ |
SLC24A3 |
NNNCAGAAGAAAGTTCGAGA |
NGG |
1 |
0.5385 |
Tier II |
| 4 |
NC_000003.12 |
3 |
197003288 |
- |
MELTF |
NNNCAGAAGGAAGCTCGCGA |
NGG |
2 |
0.1587 |
Tier II |
| 5 |
NC_000007.14 |
7 |
141357096 |
- |
TMEM178B |
NNNCAGAAGAAAGTTGGAGA |
NGG |
2 |
0.0828 |
Tier II |
| 6 |
NC_000003.12 |
3 |
197003288 |
- |
MELTF-AS1 |
NNNCAGAAGGAAGCTCGCGA |
NGG |
2 |
0.1587 |
Tier III |
| 7 |
NC_000002.12 |
2 |
142341677 |
+ |
LOC105373651 |
NNNCAGAAGAAAATTCGGGA |
NGG |
2 |
0.1231 |
Tier III |
| 8 |
NC_000005.10 |
5 |
89090310 |
- |
MEF2C-AS1 |
NNNCAGAAGAAAGTGTGCGA |
NGG |
2 |
0.0154 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
34054911 |
- |
Kalrn |
NNNCAGAAGAAAGTCCGGGA |
NGG |
2 |
0.0364 |
Tier I |
| 2 |
NC_000067.6 |
1 |
174784044 |
- |
Fmn2 |
NNNCAGAAGAAAGTTAGAGA |
NGG |
2 |
0.5385 |
Tier II |
| 3 |
NC_000080.6 |
14 |
77221763 |
- |
Enox1 |
NNNCAGAAGAAGGTTCGCTA |
NGG |
2 |
0.4815 |
Tier II |
| 4 |
NC_000069.6 |
3 |
27594546 |
+ |
Fndc3b |
NNNCAGAAGAGAGTTCTCGA |
NGG |
2 |
0.1625 |
Tier II |
| 5 |
NC_000074.6 |
8 |
120375684 |
- |
Gse1 |
NNNCAGAAGAAAGTTCTAGA |
NGG |
2 |
0.1346 |
Tier II |
Other clones with same target sequence:
(none)
