Construct: sgRNA BRDN0001146236
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCCACGGGTATTCAATACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CAD (790)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76961
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
27222538 |
+ |
CAD |
NNNACGGGTATTCAATACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
59019745 |
- |
PDE4D |
NNNTCGAGTATTCAATACAG |
NGG |
2 |
0.6364 |
Tier II |
| 3 |
NC_000005.10 |
5 |
31984120 |
+ |
PDZD2 |
NNNAAGGGTATTCAATACAC |
NGG |
2 |
0.2449 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
31059000 |
+ |
Cad |
NNNACGGGTATTCAATGCAG |
NGG |
1 |
0.1765 |
Tier I |
| 2 |
NC_000076.6 |
10 |
41240455 |
+ |
Fig4 |
NNNACAGTTATTCAATACAG |
NGG |
2 |
0.625 |
Tier II |
| 3 |
NC_000085.6 |
19 |
4714375 |
- |
Sptbn2 |
NNNACAGGTATTCAATAAAG |
NGG |
2 |
0.5385 |
Tier II |
| 4 |
NC_000082.6 |
16 |
95536789 |
- |
Erg |
NNNACGGGTATGCAGTACAG |
NGG |
2 |
0.325 |
Tier II |
| 5 |
NC_000067.6 |
1 |
70436967 |
- |
Spag16 |
NNNACGGGTATTCGATACTG |
NGG |
2 |
0.2513 |
Tier II |
| 6 |
NC_000075.6 |
9 |
114436546 |
+ |
Glb1 |
NNNACGGGTATTGAATACAG |
NGC |
2 |
0.003 |
Tier II |
| 7 |
NC_000085.6 |
19 |
4714375 |
- |
Gm42061 |
NNNACAGGTATTCAATAAAG |
NGG |
2 |
0.5385 |
Tier III |
Other clones with same target sequence:
(none)
