Construct: sgRNA BRDN0001146237
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCAAGAGTGAGCGTTACAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GALK2 (2585)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76769
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
49282025 |
+ |
GALK2 |
NNNAAGAGTGAGCGTTACAT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
54586585 |
- |
DOK5 |
NNNAAAAGTGAGAGTTACAT |
NGG |
2 |
0.3846 |
Tier II |
| 3 |
NC_000016.10 |
16 |
71806703 |
+ |
AP1G1 |
NNNATGAGTGAGCATTACAT |
NGG |
2 |
0.2727 |
Tier II |
| 4 |
NC_000005.10 |
5 |
45469919 |
+ |
HCN1 |
NNNGAGAGTGAGCCTTACAT |
NGG |
2 |
0.2679 |
Tier II |
| 5 |
NC_000003.12 |
3 |
121837274 |
- |
EAF2 |
NNNAAGAGTGAGGATTACAT |
NGG |
2 |
0.1023 |
Tier II |
| 6 |
NC_000003.12 |
3 |
138262967 |
+ |
ARMC8 |
NNNAAGAGTGAGGGTTAAAT |
NGG |
2 |
0.0734 |
Tier II |
| 7 |
NC_000003.12 |
3 |
138262967 |
+ |
NME9 |
NNNAAGAGTGAGGGTTAAAT |
NGG |
2 |
0.0734 |
Tier II |
| 8 |
NC_000010.11 |
10 |
119324809 |
+ |
GRK5 |
NNNAAGAGTGAGCGTGGCAT |
NGG |
2 |
0.0611 |
Tier II |
| 9 |
NC_000003.12 |
3 |
11383960 |
- |
ATG7 |
NNNAAGAGTGTGGGTTACAT |
NGG |
2 |
0.042 |
Tier II |
| 10 |
NC_000016.10 |
16 |
4873745 |
- |
UBN1 |
NNNAAGAGTGAGCTTTTCAT |
NGG |
2 |
0.0356 |
Tier II |
| 11 |
NC_000007.14 |
7 |
37042257 |
+ |
ELMO1 |
NNNAAGAGTGAGTGTTACAT |
NTG |
2 |
0.0273 |
Tier II |
| 12 |
NC_000002.12 |
2 |
121932279 |
- |
LOC105373592 |
NNNCAGAGTGAGCGCTACAT |
NGG |
2 |
0.0963 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
125929599 |
+ |
Galk2 |
NNNAAGAGTGAGCGCTACAT |
NGG |
1 |
0.2727 |
Tier I |
| 2 |
NC_000070.6 |
4 |
84890805 |
- |
Cntln |
NNNAAGAGTGAATGTTACAT |
NGG |
2 |
0.6533 |
Tier II |
| 3 |
NC_000084.6 |
18 |
42057872 |
- |
Sh3rf2 |
NNNAAGAGTCAGCGTTACTT |
NGG |
2 |
0.2154 |
Tier II |
| 4 |
NC_000083.6 |
17 |
73900495 |
- |
Xdh |
NNNAAGAGTGATCCTTACAT |
NGG |
2 |
0.1648 |
Tier II |
| 5 |
NC_000081.6 |
15 |
98231819 |
+ |
Olfr286 |
NNNAAGAGTGAGCGTTAGAT |
NGG |
1 |
0.1333 |
Tier II |
| 6 |
NC_000070.6 |
4 |
108610231 |
- |
Orc1 |
NNNAATAGTGAGGGTTACAT |
NGG |
2 |
0.0909 |
Tier II |
| 7 |
NC_000077.6 |
11 |
105310697 |
+ |
Mrc2 |
NNNAAGAGTGAGAGTTACAG |
NGG |
2 |
0.0679 |
Tier II |
| 8 |
NC_000070.6 |
4 |
108610231 |
- |
Gm12743 |
NNNAATAGTGAGGGTTACAT |
NGG |
2 |
0.0909 |
Tier III |
Other clones with same target sequence:
(none)
